Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia

Gužvinec, Marija; Izdebski, Radosław; Butić, Iva; Jelić, Marko; Abram, Maja; Koscak, Iva; Baraniak, Anna; Hryniewicz, Waleria; Gniadkowski, Marek; Andrašević, Arjana Tambić

doi:10.1128/aac.03116-14

Cited by 40 publications

(30 citation statements)

References 39 publications

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“…aeruginosa strains. For example, carbapenem-resistant strains were classified into several specific STs, such as ST235 (Kitao et al 2012;Edelstein et al 2013;Guzvinec et al 2014). This finding suggests that in most cases, Ps.…”

Section: Introductionmentioning

confidence: 97%

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

et al. 2016

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Section: Introductionmentioning

confidence: 97%

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

et al. 2016

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“…ST235, ST111, and ST175 are regarded as epidemic high-risk clones, and ST235 lacks pyocyanin and pyoverdine production (51). The association of ST235 with a variety of carbapenem resistance acquired by horizontal transfer of the genes such as bla PER (31,36,40,41,50), bla OXA (36,43,50), bla GES (31,39), bla SPM (36), bla VIM (31-34, 36-38, 42, 44, 52), bla IMP (36,(43)(44)(45)(46)(47)(48)(49), bla FIM (53), and bla NDM (54) suggests its importance in public health and in P. aeruginosa's flexibility in adaptation. Recently, IMP-6-producing P. aeruginosa ST235 has become endemic in Korea (48).…”

Section: Figmentioning

confidence: 99%

“…ST235 is an international clone detected in Europe (31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41), Russia (42), Asia (7,(43)(44)(45)(46)(47)(48)(49), and the Middle East (50). ST235, ST111, and ST175 are regarded as epidemic high-risk clones, and ST235 lacks pyocyanin and pyoverdine production (51).…”

Section: Figmentioning

confidence: 99%

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

Shimizu

Kayama

Kouda

et al. 2015

Antimicrob Agents Chemother

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A 9-year surveillance for multidrug-resistant (MDR) Pseudomonas aeruginosa in the Hiroshima region showed that the number of isolates harboring the metallo-␤-lactamase gene bla IMP-1 abruptly increased after 2004, recorded the highest peak in 2006, and showed a tendency to decline afterwards, indicating a history of an epidemic. PCR mapping of the variable regions of the integrons showed that this epidemic was caused by the clonal persistence and propagation of an MDR P. aeruginosa strain harboring the bla IMP-1 gene and an aminoglycoside 6=-N-acetyltransferase gene, aac(6=)-Iae in a class I integron (In113), whose integrase gene intl1 was disrupted by an IS26 insertion. Sequence analysis of the representative strain PA058447 resistance element containing the In113-derived gene cassette array showed that the element forms an IS26 transposon embedded in the chromosome. It has a Tn21 backbone and is composed of two segments sandwiched by three IS26s. In Japan, clonal nationwide expansion of an MDR P. aeruginosa NCGM2.S1 harboring chromosomally encoded In113 with intact intl1 is reported. Multilocus sequence typing and genomic comparison strongly suggest that PA058447 and NCGM2.S1 belong to the same clonal lineage. Moreover, the structures of the resistance element in the two strains are very similar, but the sites of insertion into the chromosome are different. Based on tagging information of the IS26 present in both resistance elements, we suggest that the MDR P. aeruginosa clone causing the epidemic in Hiroshima for the past 9 years originated from a common ancestor genome of PA058447 and NCGM2.S1 through an IS26 insertion into intl1 of In113 and through IS26-mediated genomic rearrangements. P seudomonas aeruginosa is an opportunistic human pathogen and a leading cause of nosocomial infections. It also causes mastitis in dairy cows as mild chronic persistent inflammation (1). Carbapenems have been used to treat P. aeruginosa infections; however, P. aeruginosa isolates resistant to carbapenems are reported in many countries and an acquired resistance to carbapenems by metallo-␤-lactamase (MBL) genes causes a serious therapeutic problem in clinical settings. Carbapenem-resistant P. aeruginosa strains producing MBLs were first discovered in Japan (2, 3) and have since been reported in many countries all over the world (4).Specifically, IMP, VIM, SPM, and NDM are the most important types of MBL with worldwide distribution, and their genes are frequently carried on class 1 integrons (4, 5). Class 1 integrons are often found on plasmids or as a part of transposons or resistance genetic elements that are able to integrate into chromosomal elements, resulting in an increase in the number of resistant Gramnegative bacilli (6). MBL-producing P. aeruginosa strains often behave as multidrug-resistant (MDR) strains. The recent increase of nosocomial infections caused by MDR P. aeruginosa has raised a serious concern.In the Hiroshima prefecture, we have conducted a multihospital surveillance for drug-resistant P. aeruginosa i...

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“…Additionally, bla GES-7 in P. aeruginosa ST111 was described previously in 2014 by Guzvinec et al (15).…”

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confidence: 99%

VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions Featuring bla _VIM-2 and bla _GES-7

Ezdra

Bado

Cordeiro

et al. 2016

Antimicrob Agents Chemother

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Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia

Abstract: 11%]). Overexpression of the natural AmpC cephalosporinase was common (42%), but only a few ST235 or ST111 isolates produced VIM-1 or VIM-2 metallo-␤-lactamases or PER-1 or GES-7 extendedspectrum ␤-lactamases.

Cited by 40 publications

References 39 publications

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions Featuring bla _VIM-2 and bla _GES-7

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Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia

Abstract: 11%]). Overexpression of the natural AmpC cephalosporinase was common (42%), but only a few ST235 or ST111 isolates produced VIM-1 or VIM-2 metallo-␤-lactamases or PER-1 or GES-7 extendedspectrum ␤-lactamases.

Cited by 40 publications

References 39 publications

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

Applying a PCR-based open-reading frame typing method for easy genotyping and molecular epidemiological analysis of Pseudomonas aeruginosa

Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions Featuring bla VIM-2 and bla GES-7

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Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS 26 Composite Transposon Carrying the bla _IMP-1 Integron in Hiroshima, Japan, 2005 to 2012

VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions Featuring bla _VIM-2 and bla _GES-7