Sequence variability of retroviral particles derived from human melanoma cells

Hirschl, Sonja; Schanab, Oliver; Seppele, Heide; Waltenberger, Andrea; Humer, Johannes; Wolff, Klaus; Pehamberger, Hubert; Muster, Thomas

doi:10.1016/j.virusres.2006.08.010

Cited by 16 publications

(10 citation statements)

References 15 publications

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“…Gel electrophoresis of RT-PCR end-products shows similar levels of POL and ENV RNAs in the four cell lines, in contrast to the dramatic differences in the case of ENV protein (data not shown). Direct sequencing of RT-PCR amplicons and NCBI BLAST analysis show that expressed sequences share 96%-98% overall homology with Group N HERV-K (Romano et al, 2006) (data not shown), as reported in other melanoma cells (Hirschl et al, 2007). Multiple endogenous and exogenous factors have been linked to the activation of HERVs including hormones, cytokines, and cytotoxic chemicals (Taruscio & Mantovani, 2004).…”

Section: Herv-k Expression Correlates With Mek and Cdk4 Activationsupporting

confidence: 52%

Simultaneous Knockdown of Mutant BRAF and Expression of INK4A in Melanoma Cells Leads to Potent Growth Inhibition and Apoptosis

Dong¹,

Chet²

2011

Treatment of Metastatic Melanoma

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Section: Herv-k Expression Correlates With Mek and Cdk4 Activationsupporting

confidence: 52%

Simultaneous Knockdown of Mutant BRAF and Expression of INK4A in Melanoma Cells Leads to Potent Growth Inhibition and Apoptosis

Dong¹,

Chet²

2011

Treatment of Metastatic Melanoma

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“…The importance of the UVR wavelength was emphasized by our experiments where the activation of HERV‐K in melanoma cells was detectable only after UVB but not after UVA irradiation. Second, the expression pattern of HERV‐K depends on the analysed melanoma cell lines (Buscher et al., 2005; Hirschl et al., 2007). Each melanoma cell line produces HERVs with a particular pattern.…”

Section: Discussionmentioning

confidence: 99%

Expression of human endogenous retrovirus K is stimulated by ultraviolet radiation in melanoma

Schanab

Humer²,

Gleiß

et al. 2011

Pigment Cell Melanoma Res

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Human endogenous retroviruses (HERVs) represent a cellular reservoir of potentially pathogenic retroviral genes. A growing body of evidence indicates that the activation of endogenous retroviral sequences might be involved in the transformation of melanocytes. In this study, we investigated the effects of ultraviolet radiation (UVR) on the expression of human endogenous retrovirus type K (HERV-K) in melanoma cells and non-melanoma cells in vitro. Solely in melanoma cell lines, irradiation with UVB (200 mJ/cm(2)) resulted in a significant transcriptional activation of the retroviral pol gene as well as in an enhanced expression of the retroviral envelope protein (env). In addition, UVB treatment induced the production of retroviral particles in the supernatants of melanoma cell lines. These data indicate that HERV-K expression can be activated by UVB irradiation and suggest an involvement of HERV-K in UVR-related melanoma pathogenesis.

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“…Moreover, particles derived from the melanoma cell line 518A2 were reported to infect bovine MDBK cells, suggesting that 518A2 cells might harbor an infectious variant of HERV-K (termed melanoma ERV [MERV]) [130]. Retroviral particles from melanoma cells contain a variety of different HERV-K RNA sequences [133]; however, a distinct MERV clone capable of infecting cell cultures in vitro has so far not been described. Also, retroviral particles derived from SK-MEL28 melanoma cells did not infect MDBK cells [110].…”

Section: Herv-k and Melanomamentioning

confidence: 99%

Endogenous retroviruses

Ruprecht

Mayer

Sauter

et al. 2008

Cell. Mol. Life Sci.

136

155

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The genomes of vertebrates contain sequences that are similar to present-day exogenous retroviruses. Such sequences, called endogenous retroviruses (ERVs), have resulted from ancestral germ line infections by exogenous retroviruses which have thereafter been transmitted in a Mendelian fashion. By analogy to exogenous tumorigenic retroviruses, ERVs have been implicated in the pathogenesis of cancer. Cumulative evidence from animal models indicates that ERVs may participate in the process of malignant transformation or promote tumor growth, e.g. through insertional mutagenesis or via counteracting tumor immunosurveillance. Here, we review the role of ERVs in tumorigenesis with focus on human ERVs (HERVs) in human cancer. Although available data suggest a potential role of HERVs in human cancers, in particular germ cell tumors, the contributions of HERVs to human tumorigenesis warrant further elucidation. (Part of a multi-author review).

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Sequence variability of retroviral particles derived from human melanoma cells

Cited by 16 publications

References 15 publications

Simultaneous Knockdown of Mutant BRAF and Expression of INK4A in Melanoma Cells Leads to Potent Growth Inhibition and Apoptosis

Simultaneous Knockdown of Mutant BRAF and Expression of INK4A in Melanoma Cells Leads to Potent Growth Inhibition and Apoptosis

Expression of human endogenous retrovirus K is stimulated by ultraviolet radiation in melanoma

Endogenous retroviruses

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