Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities

Li, Z Y; Chen, J; Lu, Jun; Wang, C R; Zhu, Xiu-feng

doi:10.4238/2015.september.25.8

Cited by 4 publications

(5 citation statements)

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“…All the examined MIC8 genes were 2055-bp long, which was confirmed by sequencing in triplicate, and their A+T content was between 50.2 and 50.6%, respectively. This is higher than that of MIC6 (Li et al, 2015c), ROP8 (Li et al, 2015a), ROP20 (Ning et al, 2015), ROP38 (Xu et al, 2014), ROP47 , HSP40 (Li et al, 2015b), and HSP60 (Lu et al, 2014), suggesting that transition (C↔T and A↔G) and transversion (A↔C, A↔T, G↔T and G↔C) might have occurred in the MIC8 gene.…”

Section: Resultsmentioning

confidence: 69%

“…After confirmation using electrophoresis on 1.0% (w/v) agarose gel, all PCR products were purified using the Wizard™ PCR-Preps DNA Purification System (Promega), ligated with pMD18-T vector (TaKaRa), and transfected into competent DH5a Escherichia coli cells (Promega). Single colonies confirmed through PCR amplification were sequenced in triplicate by Sangon Biological Engineering Biotechnology Company (Shanghai, China) Li et al, 2015a). …”

Section: Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

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Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Song

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Toxoplasma gondii is a successful opportunistic protozoan distributed worldwide, which can infect all vertebrates, leading to serious infection, blindness, and abortion. Micronemal (MIC) proteins are critically important for T. gondii infection, as they participate in various stages of the Toxoplasma life cycle, including invasion and attachment to host cells. MIC8 secretion relies on the concentration of intracellular calcium, and can mediate the invasion of T. gondii by interacting with soluble MIC3. To investigate genetic diversity of the MIC8 gene, 16 T. gondii strains from different hosts and geographical locations, and two reference isolates (ToxoDB: TGME49_245490 and TGVEG_245490) were examined in this study. The results showed that all the examined MIC8 genes are 2055 bp, with an A+T content ranging from 50.2 to 50.6%. Conversely, lower levels of variation were detected within their nucleotide and amino acid sequences. Phylogenetic analyses indicated that three classical genotypes of T. gondii and the ToxoDB#9 genotype did not group exclusively via Bayesian inference, maximum parsimony, neighbor joining, and/or maximum likelihood assays based on the nucleotide and amino acid sequences of the MIC8 gene. In summary, the T. gondii MIC8 gene is not a suitable marker for population genetic studies of this parasite.

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Section: Resultsmentioning

confidence: 69%

Section: Polymerase Chain Reaction (Pcr) Amplificationmentioning

confidence: 99%

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Song

Wang

et al. 2016

Genet. Mol. Res.

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“…Another family of ROP proteins exists that contributes to the virulence of the parasite and is involved in the membrane of the PV. Included in this family are ROP2, ROP7, ROP8 ( Hajj et al, 2007 ; Labesse et al, 2009 ; Li et al, 2015b ). Several studies have used these ROP proteins as antigens in DNA vaccine candidates.…”

Section: Resultsmentioning

confidence: 99%

Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii

Warner

Chapman

Davis

et al. 2021

Journal of Parasitology

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Toxoplasma gondii is an apicomplexan parasite that affects both humans and livestock. Transmitted to humans through ingestion, it is the second-leading cause of foodborne illness-related death. Currently, there exists no approved vaccine for humans or most livestock against the parasite. DNA vaccines, a type of subunit vaccine which uses segments of the pathogen's DNA to generate immunity, have shown varying degrees of experimental efficacy against infection caused by the parasite. This review compiles DNA vaccine efforts against Toxoplasma gondii , segmenting the analysis by parasite antigen, as well as a review of concomitant adjuvant usage. No single antigenic group was consistently more effective within in vivo trials relative to others.

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“…In addition, the phylogenetic analysis indicated that ROP8 unlike B1 gene are able to isolate the three clonal lineages, intertypes and even atypical strains of T. gondii . Although, Li et al did not achieve the desired results for the differentiation of the three classical genotype strains using the ROP8 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

“…Repetitive gene regions in the parasite genome including the Glycerol-3-phosphate dehydrogenase ( B1 ) gene, the 529 bp repeat element and the internal transcribed spacer (ITS-1) or 18S rDNA sequences are commonly used for rapid identification of T. gondii ( 12 ). However, some genetic markers, which are more polymorphic, can be used for genetic diversity and polymorphism analysis such as the rhoptry protein (ROPs) genes ( 14 – 17 ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis and Genetics Polymorphisms Evaluation of ROP8 and B1 Genes of Toxoplasma gondii in Livestock and Poul-try Hosts of Yazd, Qom and Golestan Provinces of Iran

Azimpour-Ardakan¹,

Fotouhi-Ardakani²,

Hoghooghi-Rad³

et al. 2021

IJPA

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Background: A high correlation is observed between specific clonal lineages and host types in toxoplasmosis. The main objectives of this study were comparing polymorphism and evolutionary analysis of the B1 and ROP8 genes, as well as the evaluation of phylogenic and Toxoplasma gondii isolates obtained from different hosts and regions. Methods: Overall 96 brain/ diaphragm tissue samples of livestock and poultry from three provinces of Iran (cows: 9 from Yazd, 9 from Qom; sheep: 19 from Yazd, 7 from Qom; goats: 7 from Yazd, 4 from Qom; one camel from Yazd and 37 chickens, 2 roosters and one duck from Golestan) were tested during 2018-19. A nested PCR and PCR-PCR methods were developed with the B1 and ROP8 genes. Evaluation of genetic proximity, genetic diversity and evolutionary analysis were done using MEGA-X and DnaSP5 software. Thirty samples of both genes were sequenced (18 B1 and 12 ROP8 genes), and submitted to the GenBank (MN275903-MN275932). Results: Tajima's D index analyses showed that both genes were in the negative direction of evolution. The B1 gene was more sensitive than the ROP8 gene. The ROP8 gene showed better and more acceptable results in terms of the relationship between the host and the genotyping of the samples. Conclusion: The B1 gene was only an attractive target for rapid detection of T. gondii parasites, whereas the ROP8 gene due to a high level of polymorphism was able to isolate the three clonal lineages (type I, II and III), inter-types and even atypical strains from different isolates of T. gondii.

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Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities

Cited by 4 publications

References 30 publications

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii

Phylogenetic Analysis and Genetics Polymorphisms Evaluation of ROP8 and B1 Genes of Toxoplasma gondii in Livestock and Poul-try Hosts of Yazd, Qom and Golestan Provinces of Iran

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