Sequence variation in TgROP7 gene among Toxoplasma gondii isolates from different hosts and geographical regions

Zhou, Yang; Lu, Peng; Xu, MJ; Ren, Di; Zhou, D.; Hx, Li; Lin, Rui; Zou, F.C.; Yuan, ZG

doi:10.5897/ajb12.041

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Sequence variations in the entire coding region of hsp60 among the examined T. gondii strains were 0-1.44%, which is similar to that observed in the ROP7, ROP13, and MIC13 genes Wang et al, 2012;Zhou et al, 2012). The alignment of all the 8 sequences revealed 20 variable positions, showing 1.16% overall sequence variation, which suggested a relatively considerable sequence diversity, but a lower genetic variation than that of the coding regions of the GRA5, GRA6, and AK69 genes (Fazaeli et al, 2000;Boughattas et al, 2011;Chen et al, 2012b).…”

Section: Resultssupporting

confidence: 60%

Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations

et al. 2014

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ABSTRACT. The intracellular protozoan Toxoplasma gondii is one of the most successful parasites, with the ability to invade all warmblooded animals, including humans. T. gondii heat shock protein 60 (TgHSP60) plays an important role in intracellular survival and in the differentiation of the parasite, and is also recognized as being associated with its virulence. In the present study, we examined sequence variation in the hsp60 coding region among five T. gondii isolates from different hosts and geographical regions, which were compared with the corresponding sequences of strains ME49, 76K, and GT1 available in the ToxoDB databases. The length of the T. gondii hsp60 sequence was 1728 bp for all strains, and the A+T content ranged from 41.96 to 42.13%. The sequence alignment of the 8 T. gondii strains identified 20 variable positions (0-1.44%) and showed 1.16% overall sequence 6907 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6906-6911 (2014) Sequence variation in the T. gondii hsp60 gene variation, suggesting a relatively considerable sequence diversity. Phylogenetic analysis of hsp60 sequences using Bayesian inference and maximum parsimony differentiated the two major clonal lineage types into their respective clusters, and thus separated atypical strains from classical genotypes. The results of the present study suggested that the coding region of the hsp60 gene may represent a novel genetic marker for intraspecies phylogenetic analyses of T. gondii.

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Section: Resultssupporting

confidence: 60%

Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations

et al. 2014

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“…The alignment of 10 rop17 sequences plus the corresponding sequences of the RH, PRU, and TgC7 strains available in GenBank revealed nucleotide polymorphisms at 33 positions, with an intraspecific variation of 0–2.1%. The genetic diversity in rop17 gene was higher than our previous studies for PLP1 [23], ROP7 [11], eIF4A [24], and MIC13 [25] genes and the whole genome, secretome, and kinome of T. gondii [8]. 16 variable positions were identified as transitions and the rest variable nucleotides were classified as transversions, and no deletions were detected in the 13 rop17 gene sequences.…”

Section: Resultsmentioning

confidence: 62%

“…T. gondii rhoptry protein 17 (ROP17), a member of the ROP2 subfamily [ 10 ], was predicted to have a cellular localization on the parasitophorous vacuole membrane (PVM), which may participate in the manipulation of the host signalling pathways [ 9 ]. Previous studies have shown the existence of sequence variation in some ROP genes, such as rop7 , rop9 , rop13 , and rop38 [ 11 – 14 ]. However, it is yet to be known whether sequence diversity exists in rop17 gene of T. gondii .…”

Section: Introductionmentioning

confidence: 99%

Sequence Variation inToxoplasma gondii rop17Gene among Strains from Different Hosts and Geographical Locations

Zhang

Huang

et al. 2014

The Scientific World Journal

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Genetic diversity of T. gondii is a concern of many studies, due to the biological and epidemiological diversity of this parasite. The present study examined sequence variation in rhoptry protein 17 (ROP17) gene among T. gondii isolates from different hosts and geographical regions. The rop17 gene was amplified and sequenced from 10 T. gondii strains, and phylogenetic relationship among these T. gondii strains was reconstructed using maximum parsimony (MP), neighbor-joining (NJ), and maximum likelihood (ML) analyses. The partial rop17 gene sequences were 1375 bp in length and A+T contents varied from 49.45% to 50.11% among all examined T. gondii strains. Sequence analysis identified 33 variable nucleotide positions (2.1%), 16 of which were identified as transitions. Phylogeny reconstruction based on rop17 gene data revealed two major clusters which could readily distinguish Type I and Type II strains. Analyses of sequence variations in nucleotides and amino acids among these strains revealed high ratio of nonsynonymous to synonymous polymorphisms (>1), indicating that rop17 shows signs of positive selection. This study demonstrated the existence of slightly high sequence variability in the rop17 gene sequences among T. gondii strains from different hosts and geographical regions, suggesting that rop17 gene may represent a new genetic marker for population genetic studies of T. gondii isolates.

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“…The A+T content ranged from 47.07 to 47.32%. Sequence variations in the ROP38 gene among the 13 examined T. gondii isolates were 0.2 to 1.1%, which is lower compared to the ROP7 and ROP13 genes (Wang et al, 2012;Zhou et al, 2012). Furthermore, there were 37 variable positions at the coding region of the gene, indicating very low genetic variation in the ROP38 gene.…”

Section: Resultsmentioning

confidence: 99%

Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Zhang

Chen

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Toxoplasma gondii is an obligate intracellular parasite that is able to infect almost all mammalian species, and may lead to toxoplasmosis of the host. In the present study, we examined sequence variation in rhoptry protein 38 (ROP38) genes among T. gondii isolates collected from different hosts and geographical regions. The complete ROP38 gene from 13 T. gondii isolates was amplified and sequenced. The results of sequence alignments showed that the lengths of the entire ROP38 gene ranged from 2646 to 2650 bp, with a sequence variation of 0.2-1.1%, among the 13 T. gondii isolates. This result indicated low sequence variation in the ROP38 gene. Phylogenetic analysis of ROP38 sequences using Bayesian inference showed that the clustering of the 13 T. gondii isolates was not consistent with their respective genotypes. This result indicates that the ROP38 gene is not a suitable genetic marker for population genetic studies of different T. gondii genotypes from different hosts and geographical locations, but may represent a potential vaccine candidate against toxoplasmosis, and hence worthy of further research.

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Sequence variation in TgROP7 gene among Toxoplasma gondii isolates from different hosts and geographical regions

Cited by 6 publications

References 21 publications

Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations

Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations

Sequence Variation inToxoplasma gondii rop17Gene among Strains from Different Hosts and Geographical Locations

Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

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