Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.

Saga, Yumiko; Tung, Jwu-Sheng; Shen, Francis X.; Boyse, Edward A.

doi:10.1073/pnas.83.18.6940

Cited by 67 publications

(59 citation statements)

References 19 publications

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“…Eight different isoforms of CD45 Ag may exist, generated by alternative splicing in the N-terminal extracellular domain of the molecule (7,8). The expression of different CD45 isoforms depends on the state of activation and differentiation of hemopoietic cells, and mAbs to the alternatively spliced exons have been used to separate subsets of T cells with distinct functional properties (9 -12).…”

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confidence: 99%

The Exon A (C77G) Mutation Is a Common Cause of Abnormal CD45 Splicing in Humans

Tchilian

Wallace

Imami

et al. 2001

The Journal of Immunology

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The leukocyte common (CD45) Ag is essential for normal T lymphocyte function and alternative splicing at the N terminus of the gene is associated with changes in T cell maturation and differentiation. Recently, a statistically significant association was reported in a large series of human thymus samples between phenotypically abnormal CD45 splicing and the presence of the CC chemokine receptor 5 deletion 32 (CCR5del32) allele, which confers resistance to HIV infection in homozygotes. We show here that abnormal splicing in these thymus samples is associated with the presence of the only established cause of CD45 abnormal splicing, a C77G transversion in exon A. In addition we have examined 227 DNA samples from peripheral blood of healthy donors and find no association between the exon A (C77G) and CCR5del32 mutations. Among 135 PBMC samples, tested by flow cytometric analysis, all those exhibiting abnormal splicing of CD45 also showed the exon A C77G transversion. We conclude that the exon A (C77G) mutation is a common cause of abnormal CD45 splicing and that further disease association studies of this mutation are warranted.

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confidence: 99%

The Exon A (C77G) Mutation Is a Common Cause of Abnormal CD45 Splicing in Humans

Tchilian

Wallace

Imami

et al. 2001

The Journal of Immunology

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“…CD45 Ag can potentially exist in at least eight different isoforms generated by alternative splicing in the N-terminal extracellular domain of the molecule (15,16). The expression of different CD45 isoforms depends on the state of activation and differentiation of hemopoietic cells, and in humans has been used to distinguish naive and memory T lymphocytes (17)(18)(19)(20)(21).…”

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confidence: 99%

A Deletion in the Gene Encoding the CD45 Antigen in a Patient with SCID

Tchilian

Wallace

Wells

et al. 2001

The Journal of Immunology

160

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SCID is a heterogeneous group of hereditary diseases. Mutations in the common γ-chain (γc) of cytokine receptors, including those for IL-2, IL-4, IL-7, IL-9, and IL-15, are responsible for an X-linked form of the disease, while mutations of several other genes, including Janus-associated kinase-3, may cause autosomal recessive forms of SCID. We investigated the first SCID patient to be described with minimal cell surface expression of the leukocyte common (CD45) Ag. CD45 is an abundant transmembrane tyrosine phosphatase, expressed on all leukocytes, and is required for efficient lymphocyte signaling. CD45-deficient mice are severely immunodeficient and have very few peripheral T lymphocytes. We report here that a homozygous 6-bp deletion in the gene encoding CD45 (PTPRC, gene map locus 1q31–32), which results in a loss of glutamic acid 339 and tyrosine 340 in the first fibronectin type III module of the extracellular domain of CD45, is associated with failure of surface expression of CD45 and SCID. Molecular modeling suggests that tyrosine 340 is crucial for the structural integrity of CD45 protein. This is the second description of a clinically relevant CD45 mutation, provides direct evidence for the importance of CD45 in immune function in humans, and suggests that abnormalities in CD45 expression are a possible cause of SCID in humans.

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“…Different isoforms arise through alternate RNA splicing and are found in the human, mouse, and rat (1,24,39). OX-1 antibody recognizes all isoforms (180,190,200 and 220 kDa) of the rat CD45 antigen (5).…”

Section: Discussionmentioning

confidence: 99%

“…This molecule is now classified as the leukocyte-common antigen in the rat and is homologous to human CD45 and murine T200 based on DNA sequencing data (24,31,35). CD8 as well as CD4(+) T cells are heterogenous with respect to the expression of CD45 isoforms (39).…”

Section: Abbreviationsmentioning

confidence: 99%

Monoclonal Antibody to a Structure Expressed on a Subpopulation of Rat CD8 T Cell Subsets

Nagoya

Kikuchi

Uede

1991

Microbiology and Immunology

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We have developed a monoclonal antibody, RTS-1, which can divide a rat CD8 (+) peripheral T cell population into two functionally distinct subsets. The cell-surface structure defined by this antibody is a glycoprotein with a molecular weight of 220 kDa found to be a high molecular isoform of rat CD45 antigen. CD4 (+) T cells were not stained by RTS-1 antibody. The cytotoxic T cell-enriched population did not express RTS-1 epitope on the cell surface. CD8 (+) spleen cells as well as RTS-1(+)CD8(+)T cells exhibited strong inhibition on mitogen-induced immunoglobulin G production by rat B cells. Furthermore, RTS-1 antibody, but not the control antibody, abolished CD8(+)T cell-mediated inhibition of immunoglobulin G production by rat B cells. These data suggest that RTS-1 antibody recognizes a unique determinant of rat CD45 antigen that is expressed on a fraction of CD8(+) cells.

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Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.

Cited by 67 publications

References 19 publications

The Exon A (C77G) Mutation Is a Common Cause of Abnormal CD45 Splicing in Humans

The Exon A (C77G) Mutation Is a Common Cause of Abnormal CD45 Splicing in Humans

A Deletion in the Gene Encoding the CD45 Antigen in a Patient with SCID

Monoclonal Antibody to a Structure Expressed on a Subpopulation of Rat CD8 T Cell Subsets

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