Sequences of the genes encoding the minor tip components of Pap-3 pili of Escherichia coli

Klann, Amy G.; Hull, Richard A.; Hull, Sheila I.

doi:10.1016/0378-1119(92)90071-v

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…LG1315 (iutA) [27], P678-54/pHK11 (cvaC) [67], DH5a/pCIB10B (ibeA) [23], IH11165 (bmaE, gafD) [75,76], HB101/pSR366 (kpsMT II) [62], P678-54/pAH1010 (nfaE) [77], E6468/62 (cdtB) [30], P678-54/pKT107 (traT) [78], 536-21/pANN801-13 (sfa/foc and sfaS) [79], NS24 (K1) [80], GR12 (K5) [81], and A30 (afa/dra; provided by Bogdan Nowicki). Strain P678-54 was used as a negative control [82].…”

Section: Methodsmentioning

confidence: 99%

Extended Virulence Genotypes ofEscherichia coliStrains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise

2000

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Among 75 urosepsis isolates of Escherichia coli, 29 virulence factor (VF) genes were detected by use of a novel polymerase chain reaction (PCR) assay. Compared with probe hybridization, the PCR assay's specificity was 100% and sensitivity 97.1%. fyuA (yersiniabactin: overall prevalence, 93%), traT (serum resistance, 68%), and a pathogenicity-associated island marker (71%) occurred in most strains from both compromised and noncompromised hosts. Present in <20% of strains each were sfaS, focG (F1C fimbriae), afa/dra, bmaE (M fimbriae), gafD (G fimbriae), cnf1, cdtB (cytolethal distending toxin), cvaC (colicin V), and ibeA (invasion of brain endothelium). Different VFs were variously confined to virulence-associated phylogenetic group B2 (as defined by multilocus enzyme electrophoresis); concentrated in group B2, but with spread beyond; or concentrated outside of group B2. These findings provide novel insights into the VFs of extraintestinal pathogenic E. coli and demonstrate the new PCR assay's utility for molecular epidemiological studies.

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Section: Methodsmentioning

confidence: 99%

Extended Virulence Genotypes ofEscherichia coliStrains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise

2000

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“…For comparison with predicted PapG peptide sequences from the present study, published PapG sequences for variant I from strain J96 (human source), for variant II from strains AD110, DS17, GR12, and IA2 (all human sources), and for variant III from strains J96 (human source), 83972 (human source), 1442 (canine source), and 4787 (porcine source, F165 fimbriae) were included in the PapG dendrogram (6,11,33,42,45,46,62,67). GenBank accession numbers for these sequences were X61239 (J96, allele I), M20182 (AD110), M94076 (GR12), X61237 (DS17), M20181 (IA2), X62158 (1442), X61238 (J96, allele III), AF097355 (83972), and L07092 (4787).…”

Section: Methodsmentioning

confidence: 99%

Evidence of Commonality between Canine and Human Extraintestinal PathogenicEscherichia coliStrains That ExpresspapGAllele III

et al. 2000

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Although dogs have been proposed as carriers of extraintestinal pathogenic Escherichia coli (ExPEC) with infectious potential for humans, presumed host species-specific differences between canine and human ExPEC strains have cast doubt on this hypothesis. The recent discovery that allele III of papG (the P fimbrial adhesin gene) predominates among human cystitis isolates and confers an adherence phenotype resembling that of canine ExPEC prompted the present reevaluation of the canine-human ExPEC connection. Sixteen paired pappositive urine and rectal E. coli isolates from dogs with urinary tract infection were studied. papG (adhesin) and papA (pilin) allele type, agglutination phenotypes, virulence factor genotypes, and randomly amplified polymorphic DNA and pulsed-field gel electrophoresis fingerprints were analyzed and compared with those of human ExPEC controls. The 16 canine strains contained predominantly papG allele III. Agglutination phenotypes segregated strictly according to papG allele status and were homogeneous among strains with the same papG allele profile irrespective of their human versus canine origin. Canine and human PapG variant III peptide sequences were highly homologous, without host species-specific differences. The most prevalent canine papA allele was F48, a novel variant recently identified among human urosepsis isolates. In addition to pap, human ExPEC-associated virulence genes detected among the canine strains included sfa/focDE, sfaS, fyuA, hlyA, cnf1, cdtB, kpsMT-II and -III, rfc, traT, ompT, and a marker for a pathogenicity-associated island from archetypal human ExPEC strain CFT073. Molecular fingerprinting confirmed the fecal origin of all but one canine urine isolate and showed one pair of O6 canine urine and fecal isolates to be extremely similar to an O6 human urosepsis isolate with which they shared all other genotypic and phenotypic characteristics analyzed. These data demonstrate that canine ExPEC strains are similar to, and in some instances essentially indistinguishable from, human ExPEC strains, which implicates dogs and their feces as potential reservoirs of E. coli with infectious potential for humans.

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“…Strain HU734, a lacZ derivative of acute human pyelonephritis isolate GR12, has the following properties : streptomycin and spectinomycin resistance and cysteine auxotrophy, serotype O75: K5, possession of both type 1 and P pili (the latter encoded by a single pap operon), carriage of a ColV plasmid, resistance to killing by human and mouse serum, and failure to produce haemolysin (Hagberg et al ., 1983a; Mamelak, 1994). Plasmid pAGK2 consists of a 10 kb Cla I fragment including the pap-3 operon from E. coli GR12 (HU734) inserted into the Cla I site in the tet gene of vector pACYC184 (Klann et al ., 1992; S. Hull, personal communication). The identity and purity of each culture was tested by culturing bacteria on the following media: LB medium; LB medium supplemented with ampicillin (Amp, 50 or 100 µg ml −1 ), chloramphenicol (30 µg ml −1 ), kanamycin (50 µg ml − ), streptomycin (100 µg ml −1 ) or tetracycline (25 µg ml −1 ) (as indicated by the bacterial genotype); MOPS medium with and without required amino acids (50 µg ml −1 ); TTC medium and lactose MacConkey agar.…”

Section: Methodsmentioning

confidence: 99%

Osmoregulatory transporter ProP influences colonization of the urinary tract by Escherichia coli

et al. 1998

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Osmoregulatory transporters Prop and ProU mediate the use of betaines as osmoprotectants by Escherichia coli. Glycine betaine and proline betaine are present in mammalian urines. Betaine uptake may therefore facilitate the growth of Em coli in the urinary tract, an environment of fluctuating osmolality. Prop transporter activity was approximately threefold higher in a pyelonephritis isolate, E. coli HU734, than in E. coli K-12. The growth rate of E. coli HU734 in aerated minimal salts medium was reduced twofold by 0 2 M NaCl in the absence and by 055 M NaCl in the presence of glycine betaine.Maximal growth rate stimulation was achieved when glycine betaine was added a t a concentration as low as 25 pM. Deletion of the prop locus impaired the growth rate of E. coli HU734 in human urine but not in minimal medium supplemented with NaCl (04 M), with or without glycine betaine (0-1 mM). The expression of pyelonephritis-associated (P) pili was reduced when E. coli HU734 was cultured in a rich culture medium (LB) of elevated salinity. The prop lesion had no influence on P pilus expression in witm or on the recovery of bacteria from the kidneys of inoculated mice. However, it did reduce their recovery from the bladders of inoculated mice 100-fold. These data provide the first direct evidence that osmoprotective betaine accumulation and transporter Prop are pertinent to both growth in human urine and colonization of the murine urinary tract by uropathogenic E. coli.

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Sequences of the genes encoding the minor tip components of Pap-3 pili of Escherichia coli

Cited by 8 publications

References 16 publications

Extended Virulence Genotypes ofEscherichia coliStrains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise

Extended Virulence Genotypes ofEscherichia coliStrains from Patients with Urosepsis in Relation to Phylogeny and Host Compromise

Evidence of Commonality between Canine and Human Extraintestinal PathogenicEscherichia coliStrains That ExpresspapGAllele III

Osmoregulatory transporter ProP influences colonization of the urinary tract by Escherichia coli

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