Sequences of the smallest double-stranded RNAs associated with cherry chlorotic rusty spot and Amasya cherry diseases

Covelli, L.; Kozlakidis, Zisis; Serio, Francesco Di; Çıtır, Ahmet; Açıkgöz, Serap; Hernández, Carmen; Ragozzino, A.; Coutts, Robert H.A.; Flores, Ricardo

doi:10.1007/s00705-008-0039-4

Cited by 12 publications

(12 citation statements)

References 6 publications

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“…2004; Covelli et al . 2004, 2008). However, as yet they have not been unequivocally shown to be of fungal origin (Alioto et al .…”

Section: Diversity and Incidence Of Mycovirusesmentioning

confidence: 99%

“…However, as yet they have not been unequivocally shown to be of fungal origin (Alioto et al . 2003; Covelli et al . 2008), and indeed could be plant viruses of mycovirus origin.…”

Section: Diversity and Incidence Of Mycovirusesmentioning

confidence: 99%

“…A curious situation arises with the complex of dsRNA viruses putatively infecting an unknown fungus associated with cherry Covelli et al 2004Covelli et al , 2008. However, as yet they have not been unequivocally shown to be of fungal origin (Alioto et al 2003;Covelli et al 2008), and indeed could be plant viruses of mycovirus origin.…”

Section: Host Range and Incidencementioning

confidence: 99%

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Mycoviruses of filamentous fungi and their relevance to plant pathology

Pearson

Beever

Boine

et al. 2009

Molecular Plant Pathology

446

342

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Mycoviruses (fungal viruses) are reviewed with emphasis on plant pathogenic fungi. Based on the presence of virus-like particles and unencapsidated dsRNAs, mycoviruses are common in all major fungal groups. Over 80 mycovirus species have been officially recognized from ten virus families, but a paucity of nucleic acid sequence data makes assignment of many reported mycoviruses difficult. Although most of the particle types recognized to date are isometric, a variety of morphologies have been found and, additionally, many apparently unencapsidated dsRNAs have been reported. Until recently, most characterized mycoviruses have dsRNA genomes, but ssRNA mycoviruses now constitute about one-third of the total. Two hypotheses for the origin of mycoviruses of plant pathogens are discussed: the first that they are of unknown but ancient origin and have coevolved along with their hosts, the second that they have relatively recently moved from a fungal plant host into the fungus. Although mycoviruses are typically readily transmitted through asexual spores, transmission through sexual spores varies with the host fungus. Evidence for natural horizontal transmission has been found. Typically, mycoviruses are apparently symptomless (cryptic) but beneficial effects on the host fungus have been reported. Of more practical interest to plant pathologists are those viruses that confer a hypovirulent phenotype, and the scope for using such viruses as biocontrol agents is reviewed. New tools are being developed based on host genome studies that will help to address the intellectual challenge of understanding the fungal-virus interactions and the practical challenge of manipulating this relationship to develop novel biocontrol agents for important plant pathogens.

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“…2004; Covelli et al . 2004, 2008). However, as yet they have not been unequivocally shown to be of fungal origin (Alioto et al .…”

Section: Diversity and Incidence Of Mycovirusesmentioning

confidence: 99%

“…However, as yet they have not been unequivocally shown to be of fungal origin (Alioto et al . 2003; Covelli et al . 2008), and indeed could be plant viruses of mycovirus origin.…”

Section: Diversity and Incidence Of Mycovirusesmentioning

confidence: 99%

Section: Host Range and Incidencementioning

confidence: 99%

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Mycoviruses of filamentous fungi and their relevance to plant pathology

Pearson

Beever

Boine

et al. 2009

Molecular Plant Pathology

446

342

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“…An important argument in favour of our conclusion that the 1.5 and 1.58 kbp dsRNAs in pine probably represent the genomic dsRNAs of a new cryptovirus is the phylogenetic relationship between pine RdRp and the plant cryptoviruses BCV3 and P. pyrifolia virus. However, we cannot entirely exclude the possibility that the pine RdRp [30] might belong to a mycovirus and/or have a fungal origin, because literature data presenting phylogenetic analysis of the RdRp of cryptic viruses and especially of Alphacryptoviruses do not seem to show that these viruses form a phylogenetically coherent ensemble. Therefore, as suggested by Boccardo and Candresse [7,8], a re-examination of the taxonomy of these viruses might be needed.…”

Section: Discussionmentioning

confidence: 88%

Searching for a New Putative Cryptic Virus in Pinus sylvestris L

et al. 2006

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Double-stranded RNAs (dsRNAs) were detected in different pine populations in Germany and Hungary. Two dsRNA species of 1.5 and 1.58 kbp, respectively, persisted in the same trees for at least 2 years and their presence was not associated with any symptoms. The dsRNAs were found to sediment in the VLP (virus-like particles) fraction and to be protected by protein(s) against RNase A digestion at low salt. cDNA cloning and sequencing of the smaller segment (dsRNA2) led to the identification of a putative RNA-dependent RNA-polymerase (RdRp) containing the GDD, as well as three other, conserved motifs. Sequence comparison with different RNA viruses and phylogenetic analysis indicates that the putative RdRp from pine shows highest similarity to the homologous proteins of Beet cryptic virus 3 and of a cryptic virus of Pyrus pyrifolia. On the basis of these results we suggest that the 1.5 and 1.58 kbp dsRNAs in P. sylvestris may represent the genomic segments of a new plant cryptic virus, Cryptoviruses have not yet been reported to occur in Gymnosperms.

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“…For instance, the molecular characteristics of mycoviral double-stranded RNAs (dsRNAs) normally hold clues not only to their aetiology but also their own genesis. For example, in plants, two cherry diseases viz., the Amasya Cherry Disease (ACD) and Cherry Chlorotic Rusty Disease (CCRD) observed in Italy or Turkey, are two recent disorders that are likely to be of fungal aetiology, but are also concurrently associated with complex pattern of mycoviral like 10-12 dsRNAs Covelli et al, 2004). Further studies by the group (Kozlakidis et al, 2006) revealed that while the larger dsRNAs carrying deduced RNA dependent RNA Polymerase (RDRP) sequences (responsible for replication process) are homologous to others in Totoviridae group, their relationships either to and the sequences of the smaller dsRNAs and of their role in replication details are not fully decoded, rendering at present, these viruses as novel.…”

Section: Introductionmentioning

confidence: 99%

The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

Rao

Nelson²,

McClean³

2007

Current Issues in Molecular Biology

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New variants of pathogenic fungal viruses are emerging and they are enigmatic in revealing their molecular identity and of their origin. Double-stranded RNAs, some in non-encapsidated forms are increasingly becoming causal agents for sporadic diseases and are consistently associated with a complex profile of dsRNAs, presumably of (multiple) viral origin present in the host while the same are conspicuously absent in healthy (looking)

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Sequences of the smallest double-stranded RNAs associated with cherry chlorotic rusty spot and Amasya cherry diseases

Cited by 12 publications

References 6 publications

Mycoviruses of filamentous fungi and their relevance to plant pathology

Mycoviruses of filamentous fungi and their relevance to plant pathology

Searching for a New Putative Cryptic Virus in Pinus sylvestris L

The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

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