Sequencing and analysis of genomes of bacteria used to protect plants and animals from diseases

Site-specific markerless inactivation of genes lysR3 and mtfA of bacteria Pseudomonas brassicacearum S-1 was carried out. It was shown that strains P. brassicacearum S-1-lysR3, P. brassicacearum S-1-mtfA and P. brassicacearum S-1-lysR3-mtfA demonstrate increased antagonistic activity against a number of phytopathogens 1.2–1.6 times higher compared with the original strain. In the laboratory experiment, mutant variants did not affect growth and development of tomato, cucumber and parsley plants. Treating tomato and cucumber seeds with a solution of the culture of the strain P. brassicacearum S-1-lysR3-mtfA led to the increase of seedlings roots length by 1.4 times compared with the original strain. We also determined that the examined strains were able to survive in the soil microbial consortium.

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Increasing the antimicrobial activity of Pseudomonas brassicacearum S-1 by inactivation of lysR3 and mtfA genes

Muratova

Mandryk-Litvinkovich²,

Валентович

2021

Journal of the Belarusian State University. Biology

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Sequencing and analysis of genomes of bacteria used to protect plants and animals from diseases

Abstract: The main stages of work with perspective strains for protecting plants and animals from diseases are presented: genome sequencing and analysis, genetic construction of improved strains and checking their survival under model conditions.

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Increasing the antimicrobial activity of Pseudomonas brassicacearum S-1 by inactivation of lysR3 and mtfA genes

Increasing the antimicrobial activity of Pseudomonas brassicacearum S-1 by inactivation of lysR3 and mtfA genes

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