Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)

Abstract

In this study, the complete mitogenome sequence of Rugosa emeljanovi (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of R. emeljanovi contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 28.1% A, 26.8% C, 14.8% G, and 30.3% T. Mitochondrial genome analyses based on the NJ method yield phylogenetic trees, including the 13 already reported Ranidae family frogs. The molecular data presented here provide a useful tool for systematic analyses of genus Rugosa and Glandirana.

The complete mitochondrial genome ofPyxicephalus adspersus: high gene rearrangement and phylogenetics of one of the world’s largest frogs

“…BI and ML analyses were performed with 59 anuran mt genomes including Pyxicephalus adspersus . In total this included 55 species as the ingroup from Ranidae, Dicroglossidae, Rhacophoridae, Mantellidae, Pyxicephalidae, Petropedetidae, Ptychadenidae, Ceratobatrachidae, and Phrynobatrachidae (Alam et al, 2010; Chen et al, 2011; Hofman et al, 2012; Huang et al, 2016a, 2016b; Jiang et al, 2017; Kakehashi et al, 2013; Kurabayashi et al, 2006, 2010; Li et al, 2014b, 2016b; Lin et al, 2014; Liu, Wang & Zhao, 2017a; Liu et al, 2017b; Liu, Wang & Su, 2005; Ni et al, 2015; Ren et al, 2009; Sano et al, 2004, 2005; Xia et al, 2014; Yan et al, 2016; Yang et al, 2018; Yu, Zhang & Zheng, 2012a; Yu et al, 2012b, 2015; Zhang, Xia & Zeng, 2016; Zhang et al, 2005, 2009, 2013, 2018a; Zhao, Meng & Su, 2018; Zhou et al, 2009) and four species as outgroups from Microhylidae (Chen et al, 2016; Wang et al, 2018; Zhao, Meng & Su, 2018). We used the nucleotide data to assess BI and ML topology to discuss the phylogenetic position of Pyxicephalus .…”

Peer Review #1 of "The complete mitochondrial genome of Pyxicephalus adspersus: high gene rearrangement and phylogenetics of one of the world’s largest frogs (v0.2)"

“…BI and ML analyses were performed with anuran mt genomes including P. adspersus. In total this included species as the ingroup from Ranidae, Dicroglossidae, Rhacophoridae, Mantellidae, Pyxicephalidae, Petropedetidae, Ptychadenidae, Ceratobatrachidae and Phrynobatrachidae (Alam et al, 2010;Chen et al, 2011;Hofman et al, 2012;Huang et al, 2016a;Huang et al, 2016b;Jiang et al, 2017;Kakehashi et al, 2013;Kurabayashi et al, 2006;Kurabayashi et al, 2010;Li et al, 2016b;Li et al, 2014b;Lin et al, 2014;Liu et al, 2017a;Liu et al, 2017b;Liu et al, 2005;Ni et al, 2015;Ren et al, 2009;Sano et al, 2004;Sano et al, 2005;Xia et al, 2014;Yan et al, 2016;Yang et al, 2018;Yu et al, 2015;Yu et al, 2012a;Yu et al, 2012b;Zhang et al, 2016;Zhang et al, 2018a;Zhang et al, 2009;Zhang et al, 2013;Zhang et al, 2005;Zhao et al, 2018;Zhou et al, 2009) and four species as outgroups from Microhylidae (Chen et al, 2016;Zhao et al, 2018). We used the nucleotide data to assess BI and ML topology to discuss the phylogenetic position of Pyxicephalus.…”