Sequencing, cloning and expression of the dsz genes required for dibenzothiophene sulfone desulfurization from Gordonia alkanivorans strain 1B

Alves, Luís Manuel; Melo, M. F. De; Mendonça, Diogo; Simões, Fernanda; Matos, José; Tenreiro, Rogério; Gı́rio, Francisco M.

doi:10.1016/j.enzmictec.2006.11.008

Cited by 47 publications

(28 citation statements)

References 25 publications

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“…Thus it is possible that the presumed three genes may not be present in an operon form in Eu-32, and each gene may have an independent promoter at 5 0 end. Moreover, it may also be possible that the DNA sequences for presumed dszB gene of isolate Eu-32 are significantly different from those previously described sequences upon which we based our primer designs (Piddington et al 1995;Ishii et al 2000;Kirimura et al 2004;Denome et al 1994;Alves et al 2007). Indeed Lee and co-workers (2006) observed that proteins having homology with DszB are often found in genomic contexts that differ from the dsz operon in many microbial species hinting at the presence of as yet to be discovered sulphur metabolic pathways.…”

Section: Discussionmentioning

confidence: 80%

Phylogenetic characterization and novelty of organic sulphur metabolizing genes of Rhodococcus spp. (Eu-32)

et al. 2014

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Rhodococcus spp. (Eu-32) has the unique ability to metabolize organic sulphur containing compounds like dibenzothiophene through an extended sulphur specific pathway (Akhtar et al., in FEMS Microbiol Lett 301:95-102, 2009). Efforts were made to isolate and characterize the presumed desulphurizing genes (dszABC) involved in the sulphur specific pathway of isolate Eu-32 by employing standard and degenerate polymerase chain reaction primers. The partial dszA gene sequence of isolate Eu-32 showed 92% sequence identity with a putative FMNH-2 dependent monooxygenase of Rhodococcus erythropolis PR4. The dszC gene sequence showed 99% homology with the dibenzothiophene monooxygenase desulphurizing enzyme of another Rhodococcus species. The dszB gene was not unambiguously identified. A phylogenetic analysis by maximum likelihood method of the 16S rRNA gene and deduced DszA and C amino acid sequences suggest that horizontal gene transfer events might have taken place during the evolution of desulphurizing genes of Rhodococcus spp. (Eu-32).

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Section: Discussionmentioning

confidence: 80%

Phylogenetic characterization and novelty of organic sulphur metabolizing genes of Rhodococcus spp. (Eu-32)

et al. 2014

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“…This bacterium was cultured in sulfur-free mineral (SFM) medium supplemented with a trace element solution as described by Alves et al [1]. For the assays in which a single metal ion was removed, the culture medium was prepared adding the metal compounds separately instead of adding the trace element solution.…”

Section: Methodsmentioning

confidence: 99%

Evidence for the role of zinc on the performance of dibenzothiophene desulfurization by Gordonia alkanivorans strain 1B

Alves¹,

Matos²,

Tenreiro³

et al. 2007

J Ind Microbiol Biotechnol

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Gordonia alkanivorans strain 1B is able to desulfurize dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway. However, both the cell growth and the rate of desulfurization can be largely affected by the nutrient composition of the growth medium due to cofactor requirements of many enzymes involved in the biochemical pathways. In this work, the effect of several metal ions on the growth and DBT desulfurization by G. alkanivorans was studied. From all the metal ions tested, only the absence of zinc significantly affected the cell growth and the desulfurization rate. By increasing the concentration of Zn from 1 to 10 mg L(-1), 2-HBP productivity was improved by 26%. The absence of Zn(2+), when sulfate was also used as the only sulfur source, did not cause any difference in the bacterial growth. Resting cells grown in the presence of Zn(2+) exhibited a 2-HBP specific productivity of 2.29 micromol g(-1) (DCW) h(-1), 7.6-fold higher than the specific productivity obtained by resting cells grown in the absence of Zn(2+) (0.30 micromol g(-1) (DCW) h(-1)). These data suggests that zinc might have a key physiological role in the metabolism of DBT desulfurization.

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“…The transformed cells having a transformation efficiency of 1.65 × 10 5 CFUs μg − 1 plasmid DNA exhibited a 2.67-fold increase in desulphurization activity when compared to wild type cells, even in the presence of inorganic sulphate [107]. Still other studies showed the plasmid pUC19 to be a carrier and vector for the desulphurization genes of G. alkalivorans 1B [108].…”

Section: Genetic Modificationmentioning

confidence: 96%

Biodegradation of dibenzothiophene and its application in the production of clean coal

Mishra

Pradhan

Panda

et al. 2016

Fuel Processing Technology

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Sequencing, cloning and expression of the dsz genes required for dibenzothiophene sulfone desulfurization from Gordonia alkanivorans strain 1B

Cited by 47 publications

References 25 publications

Phylogenetic characterization and novelty of organic sulphur metabolizing genes of Rhodococcus spp. (Eu-32)

Phylogenetic characterization and novelty of organic sulphur metabolizing genes of Rhodococcus spp. (Eu-32)

Evidence for the role of zinc on the performance of dibenzothiophene desulfurization by Gordonia alkanivorans strain 1B

Biodegradation of dibenzothiophene and its application in the production of clean coal

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