Sequencing human rhinoviruses: Direct sequencing versus plasmid cloning

Linder, Jodell E.; Plachco, Tatyana E.; Libster, Romina; Miller, E. Kathryn

doi:10.1016/j.jviromet.2014.09.020

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…42 Samples positive for RV were then cloned and sequenced. A 548-nucleotide sequence that encompassed the VP4/VP2 region 51 was amplified, directly sequenced, 45 and compared with published GenBank sequences to determine species. MPV and RSV were tested for by RT-PCR using primers/probes previously described.…”

Section: Samplesmentioning

confidence: 99%

Hospitalizations and outpatient visits for rhinovirus-associated acute respiratory illness in adults

Miller

Linder

Kraft

et al. 2016

Journal of Allergy and Clinical Immunology

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Background Rhinovirus is linked to asthma exacerbations and chronic obstructive pulmonary disease exacerbations in adults. The severity and rates of rhinovirus acute respiratory illnesses (ARI) in adults are uncertain. Objectives We determined rhinovirus-associated ARI rates in adults presenting for care in multiple settings and identified factors associated with rhinovirus detection. Methods This prospective, population-based cohort enrolled Tennessee residents ≥18 years old in the emergency department (ED), outpatient clinics, or hospitalized for ARI December 2008-May 2010. Nasal/throat swabs were collected and tested for rhinovirus and other viruses by RT-PCR. Rates of ED visits and hospitalizations were calculated and rhinovirus-positive and -negative patients were compared. Results Among 2351 enrollees, rhinovirus was detected in 247 (11%). There were 7 rhinovirus-associated ED visits and 3 hospitalizations per 1000 adults annually. Patients with rhinovirus, compared to virus-negative ARI, were more likely to present with wheezing (odds ratio [OR] 1.7, 95% confidence interval [CI] 1.23-2.35, p<0.001), to be a current smoker (OR 2.31, CI 1.68-3.19) or live with a smoker (OR 1.72, CI 1.10-2.67), have a history of chronic respiratory disease (OR 1.61, CI 1.17-2.22), and were less likely to be hospitalized versus seen in the outpatient setting (OR 0.58, CI 0.41-0.83). Conclusion Rhinovirus is associated with a substantial number of ED visits and hospitalizations for ARI in adults. There may be modifiable factors that can reduce the likelihood of presenting with rhinovirus-associated ARI.

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Section: Samplesmentioning

confidence: 99%

Hospitalizations and outpatient visits for rhinovirus-associated acute respiratory illness in adults

Miller

Linder

Kraft

et al. 2016

Journal of Allergy and Clinical Immunology

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“…It should be noted that previous research has reported failure rates for both methods. 45 Therefore, the selection of the most efficient and cost-effective approach to investigate the methylation pattern depends on the study question and design, as well as the information provided by each method. In this study, we used a site-specific assessment method, facilitating a rapid and direct investigation of the methylation status.…”

Section: Gene Symbol (Ensembl Accession Number)mentioning

confidence: 99%

A procedure for DNA methylation assessment in osteoporosis-related gene promoters of umbilical cord blood: A study on the Prospective Epidemiological Research Studies in Iran (PERSIAN) birth cohort

Baradaran Mahdavi,

Javadirad,

Rafieian

et al. 2024

Bioimpacts

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Background: This study aimed to investigate the feasibility of preparation of sustained-release chlorpheniramine maleate (CPM) pellets based on Compritol® as a lipid matrix and evaluation of the affecting factors on pellet properties. Method: Using the D-optimal experimental design, different pellet formulations containing various amounts of CPM, Compritol® and Avicel were prepared by the wet extrusion-spheronization method. Then the pellets were cured at 40, 65 and 90 ̊C for 4 and 8 h to study the effect of the thermal process. The physicomechanical properties of the pellets were investigated in terms of particle size distribution, pelletization yield, mechanical strength, aspect ratio and sphericity. To investigate the possible interaction of CPM and Compritol®, as well as to evaluate the morphology and surface characteristics of the pellets DSC and SEM were used, respectively. Also, to investigate the drug release rate from pellets the dissolution test was carried out and mean dissolution time (MDT) was calculated to compare different formulations. Results: The results showed that the curing process up to 65 °C improves the strength of the pellets. However, increasing the curing temperature from 65 to 90 °C and also increasing the curing time from 4 to 8 h did not have a significant effect on the strength of the pellets but increased the drug release rate of pellets. Increasing the amount of the drug or decreasing Compritol® in the matrix of pellets leads to a larger particle size with greater mechanical strength. All formulations of the pellets had an aspect ratio and sphericity of about 1.1 and 0.9 respectively, which indicates the spherical shape of the pellets as shown by SEM. DSC thermograms indicate the reduction of the crystallinity or the change of the crystalline form of the drug to amorphous during the pelletization process. Conclusion: The results revealed the feasibility of preparing lipid-based sustained-release matrix pellets using the wet extrusion-spheronization method. The optimal formulation in terms of physicomechanical properties and release rate was the formulation containing 8% CPM, 67% Compritol® and 25% Avicel, which were dried at 40 ° C for 4h and released about 90% of the drug within 12 h.

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Sequencing human rhinoviruses: Direct sequencing versus plasmid cloning

Cited by 2 publications

References 30 publications

Hospitalizations and outpatient visits for rhinovirus-associated acute respiratory illness in adults

Hospitalizations and outpatient visits for rhinovirus-associated acute respiratory illness in adults

A procedure for DNA methylation assessment in osteoporosis-related gene promoters of umbilical cord blood: A study on the Prospective Epidemiological Research Studies in Iran (PERSIAN) birth cohort

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