Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

Abstract

Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing.

Epidemiology of Methicillin-Resistant Staphylococcus aureus in Slovakia, 2020 – Emergence of an Epidemic USA300 Clone in Community and Hospitals

“…Independently of PVL positivity, CA-MRSA characteristics, such as a younger age and an outpatient setting, were associated with CC5-MRSA-I (t010, t045), CC5-MRSA-IV (t311, t179, t3235), and CC45-MRSA-IV (t015, t026, t330, t671, t1231), but, of these, only the 11 CC5-MRSA-IV (9× t311; 2× t3235) isolates were positive for PVL. These lineages, found in between 9.95% (41/412) and 13.11% (54/412) of isolates, are rarely, if ever, found in hospital-based studies in the Czech Republic, Poland, and Austria ( 9 , 11 , 13 , 14 , 25 – 27 ), stressing that focusing only on hospital epidemiology could significantly underestimate the presence of strains that are circulating in the community and causing significant disease burden.…”

Mini-Multilocus Sequence Typing Scheme for the Global Population of Neisseria gonorrhoeae

“…An alternative and less labor-intensive method of genotyping is the MLST-based scheme, which predicts genotypes by analyzing informative single-nucleotide polymorphisms (mini-MLST) [ 23 , 24 ]. Due to the high conservatism of the genes used for MLST, polymorphisms are typically found in specific positions.…”