1993
DOI: 10.1111/j.1365-2958.1993.tb00987.x
|View full text |Cite
|
Sign up to set email alerts
|

Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL‐protein reveals an unusual degree of internal homology

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

2
45
0
1

Year Published

1994
1994
2011
2011

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 39 publications
(48 citation statements)
references
References 3 publications
2
45
0
1
Order By: Relevance
“…It was hypothesized that the cooperative degradation of crystalline cellulose involved an interaction between S S (and presumably other cellulases), S L and the insoluble substrate. S L (an anchorage subunit) would function to bind S S (and other catalytic proteins of the complex) to the cellulose surface in a manner optimal for hydrolysis [111,112], consistent with the "anchor-enzyme" hypothesis [38]. The anchor-enzyme model was further conWrmed using recombinant forms of S S and S L [63].…”
Section: Gram-positive Bacterial Anaerobesmentioning
confidence: 89%
See 1 more Smart Citation
“…It was hypothesized that the cooperative degradation of crystalline cellulose involved an interaction between S S (and presumably other cellulases), S L and the insoluble substrate. S L (an anchorage subunit) would function to bind S S (and other catalytic proteins of the complex) to the cellulose surface in a manner optimal for hydrolysis [111,112], consistent with the "anchor-enzyme" hypothesis [38]. The anchor-enzyme model was further conWrmed using recombinant forms of S S and S L [63].…”
Section: Gram-positive Bacterial Anaerobesmentioning
confidence: 89%
“…The scaVoldin also contains dockerins (type II) that anchor the cellulosomes to the cell surface. Much information on the scaVoldin structure was revealed by the genetic sequencing of the gene by Gerngross et al [38]. Cellulosomes are bound to the cell surface during early log phase of growth and are released into the medium during late log phase.…”
Section: Gram-positive Bacterial Anaerobesmentioning
confidence: 99%
“…13 The proximity effect was estimated using a hybrid scaffoldin called Scaf4, devoid of family 3a CBM and containing only one C. thermocellum and one C. cellulolyticum connected by a 50 residue linker. 10 The latter encompasses the typical C. thermocellum 39 residue linker found in the scaffoldin CipA, 14 and the characteristic intercohesins linker of 11 residues observed in the C. cellulolyticum scaffoldin CipC. 8 The binding of various C. cellulolyticum cellulase pairs onto Scaf4 induced significant increase in the activity towards crystalline cellulose.…”
Section: Introductionmentioning
confidence: 99%
“…It comprises a cellulose-specific carbohydrate-binding module, which targets the enzyme complex to its substrate, nine type-I cohesin (CohI) modules, an X module, and a C-terminal type-II dockerin (DocII) module. 6,7 The CohI modules mediate the integration of the catalytically active subunits into the complex via a high-affinity, noncovalent interaction with the enzyme-associated type-I dockerin (DocI) modules, [8][9][10] whereas the DocII module associates the cellulosome complex to the bacterial cell surface via binding to the CohII modules of the cell surface anchoring subunits SdbA, Orf2p, and OlpB. 11,12 Substantial efforts have been undertaken to elucidate the tertiary and quaternary structural features of the cellulosome.…”
mentioning
confidence: 99%