Sequencing of individual chromosomes of plant pathogenic Fusarium oxysporum

Kashiwa, Takeshi; Kozaki, Toshinori; Ishii, Kazuo; Turgeon, B. Gillian; Teraoka, Tohru; Komatsu, Ken; Arie, Tsutomu

doi:10.1016/j.fgb.2016.12.001

Cited by 15 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). 30,31) Additionally, in the last few years, with the remarkable progress of SMRT ® sequencing technology, comparative genome analyses among strains have become easy, and even in a small laboratory like ours, genome sequencing can be used as a research tool. In fact, twelve supercontigs were obtained as a result of the SMRT ® sequencing of a banana Panama disease fungus (F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

Arie

2019

J. Pestic. Sci.

Self Cite

109

View full text Add to dashboard Cite

Fusarium diseases are significant hindrances to food plant production and are very difficult to control, especially soilborne diseases caused by F. oxysporum. First I outline the Fusarium diseases and introduce examples of the recent outbreak of Fusarium diseases in Japan. Then I summarize my studies on (1) the control of Fusarium diseases by biological agents and by inducing resistance to diseases in plants, (2) the specific detection of forms and races in F. oxysporum using immunological measures and molecular measures based on phylogeny and pathogenicity-determining genes, and (3) molecular and genetic studies on Fusarium diseases, including evolutionary, genetic, and genomic analyses of the emergence and divergence of forms and races in F. oxysporum.

show abstract

Section: Discussionmentioning

confidence: 99%

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

Arie

2019

J. Pestic. Sci.

Self Cite

109

View full text Add to dashboard Cite

show abstract

“…Chromosome DNA recovery from CHEF gels were performed according to the method described previously (72). Chromosome bands of interest were excised from the gel and were placed in 2 ml microcentrifuge tubes, then heated at 100°C while shaking at 350 rpm for at least 10 minutes to melt the agarose.…”

Section: Methodsmentioning

confidence: 99%

A partial pathogenicity chromosome inFusarium oxysporumis sufficient to cause disease and can be horizontally transferred

Fokkens

Conneely

et al. 2020

Preprint

View full text Add to dashboard Cite

During host colonization, plant pathogenic fungi secrete proteins, called effectors, to facilitate infection. Collectively, effectors may defeat the plant immune system, but usually not all effectors are equally important for infecting a particular host plant. In Fusarium oxysporum f.sp. lycopersici, all known effector genes – also called SIX genes – are located on a single accessory chromosome which is required for pathogenicity and can also be horizontally transferred to another strain. To narrow down the minimal region required for virulence, we selected partial pathogenicity chromosome deletion strains by fluorescence-assisted cell sorting of a strain in which the two arms of the pathogenicity chromosome were labelled with GFP and RFP, respectively. By testing the virulence of these deletion mutants, we show that the complete long arm and part of the short arm of the pathogenicity chromosome are not required for virulence. In addition, we demonstrate that smaller versions of the pathogenicity chromosome can also be transferred to a non-pathogenic strain and they are sufficient to turn the non-pathogen into a pathogen. Surprisingly, originally non-pathogenic strains that had received a smaller version of the pathogenicity chromosome were much more aggressive than recipients with a complete pathogenicity chromosome. Whole genome sequencing analysis revealed that partial deletions of the pathogenicity chromosome occurred mainly close to repeats, and that spontaneous duplication of sequences in accessory regions is frequent both in chromosome deletion strains and in horizontal transfer (recipient) strains.Author SummaryFungal genomes can often be divided into a core genome, which is essential for growth, and an accessory genome which is dispensable. The accessory genome in fungi can be beneficial under some conditions. For example, in some plant-pathogenic fungi, virulence genes are present in the accessory genome, which enable these fungi to cause disease on certain hosts. In Fusarium oxysporum f.sp. lycopersici, which infects tomato, all host-specific virulence genes are located on a single accessory chromosome. This ‘pathogenicity chromosome’ can be horizontally transferred between strains. Here, we found that many suspected virulence genes are in fact not required for virulence because strains without a large part of the pathogenicity chromosome, including these genes, showed no reduced virulence. In addition, we demonstrate that partial pathogenicity chromosomes can be horizontally transferred to a non-pathogen. Surprisingly, originally non-pathogenic strains that had received a partial pathogenicity chromosome were more virulent than strains that had received the complete pathogenicity chromosome.

show abstract

“…Thanks to techniques including PCR, sequencing using ABI sequencer, gene-tagging by the REMI method, gene-disruption, and gene-expression analyses, my study of molecular mechanisms of pathogenicity, evolution of pathogenicity, host determination and mating has greatly progressed (Arie et al 1991 , 1995 ). Regarding disease diagnosis, I worked to establish detection techniques for soil pathogens using immunological methods in the 1990s, and since 2000, we have been able to rapidly detect specific soil pathogens and their races using PCR, real-time PCR, and LAMP™ (Hirano and Arie 2006 ; Inami et al 2010 ; Kashiwa et al 2017 ). In the past several years, based on the results of comparative genomic analyses of isolates, we also established a technology for the specific detection of race-determining SNPs (Arie 2010 , 2019 ; Ayukawa et al 2017 , 2018 ).…”

Section: From "The World Of the Loupe" To The World Of Genomic Sciencmentioning

confidence: 99%

Sequencing of individual chromosomes of plant pathogenic Fusarium oxysporum

Cited by 15 publications

References 19 publications

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

A partial pathogenicity chromosome inFusarium oxysporumis sufficient to cause disease and can be horizontally transferred

A new era in plant pathology in Japan: incorporation of the Phytopathological Society of Japan and research reform directed by genomic studies

Contact Info

Product

Resources

About