Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Onda, Mitsuhiko; Lvov, Yuri; Ariga, Katsuhiko; Kunitake, Toyoki

doi:10.1002/(sici)1097-0290(19960720)51:2<163::aid-bit5>3.0.co;2-h

Cited by 249 publications

(67 citation statements)

References 11 publications

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“…biotin-streptavidin, have also been exploited to fabricate a wide array of protein multilayer architectures 3) . More recently, a layerby-layer approach has been utilized to fabricate protein multilayers via the assembly of proteins and oppositely charged polyelectrolytes [4][5][6][7][8][9][10] . This general and versatile method has been employed to incorporate numerous water-soluble macromolecular species in polyelectrolyte films 11) .…”

Section: Introductionmentioning

confidence: 99%

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Preparation of enzyme multilayers on colloids for biocatalysis

Schüler

Caruso

2000

Macromol. Rapid Commun.

117

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The construction of enzyme multilayer films on colloidal particles for biocatalysis is described. The enzyme multilayers were assembled on submicrometer‐sized polystyrene spheres via the alternate adsorption of poly(ethyleneimine) and glucose oxidase using a layer‐by‐layer approach. Microelectrophoresis and single particle light scattering measurements revealed regular and step‐wise assembly of the multilayers on the colloids. The high surface area bio‐multilayer coated particles formed were subsequently utilized in enzymatic catalysis.

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Section: Introductionmentioning

confidence: 99%

“…This general and versatile method has been employed to incorporate numerous water-soluble macromolecular species in polyelectrolyte films 11) . Multilayers of a number of functional bio-species have been produced on planar substrates, and their enzymatic and immunosensing potential have been demonstrated [5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

Preparation of enzyme multilayers on colloids for biocatalysis

Schüler

Caruso

2000

Macromol. Rapid Commun.

117

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“…Earlier reports indeed show that enzymes, like glucose oxidase and glucoamylase, embedded in LbL films preserve not only their secondary structure [20,21] but also their enzymatic activity. [17][18][19] However, the efficiency of the reactions depends strongly on the enzyme and the nature of the multilayers. [19] …”

Section: Enzymatic Cleavage Of P1-loaded Pem Beadsmentioning

confidence: 99%

“…[17][18][19] However, the efficiency of the reactions depends strongly on the enzyme and the nature of the multilayers. [19] …”

Section: Enzymatic Cleavage Of P1-loaded Pem Beadsmentioning

confidence: 99%

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Evaluation of Encoded Layer‐By‐Layer Coated Microparticles As Protease Sensors

Stubbe

Gevaert

Derveaux

et al. 2008

Adv Funct Materials

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Proteases are important pharmaceutical targets for new drugs because of their involvement in numerous disease processes. This study evaluates whether photophysically encoded microparticles carrying fluorescently labeled protease substrates (peptides) at their surface show potential for detecting proteases in a sample. Layer‐by‐layer (LbL) polyelectrolyte coatings, containing a red‐labeled peptidic trypsin substrate, are carefully designed and applied at the surface of the encoded microparticles. The peptide‐loaded LbL coatings lose their red fluorescence upon incubation in a trypsin solution, indicating that LbL‐coated microparticles show potential to screen for the presence of active proteases in biological samples.

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Stepwise polyelectrolyte assembly on particle surfaces: a novel approach to colloid design

Sukhorukov

Donath

Davis

et al. 1998

Polym. Adv. Technol.

640

490

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Polyelectrolyte multilayers were deposited onto polystyrene and melamine formaldehyde latex particles by means of consecutive adsorption. Two different methods of multilayer growth were employed. First, adsorption of polyelectrolytes at a concentration exceeding saturation amounts was combined with the removal of the nonbound polyelectrolyte by means of centrifugation. Second, adsorption of polyelectrolyte was performed at a concentration just sufficient for saturation coverage. Both methods yielded continuous layer growth. The process of film formation was followed by electrophoresis, dynamic light scattering, single particle light scattering and fluorescence intensity measurements. Layer deposition onto partially crosslinked melamine resin latex particles, which were soluble at pH values of less than 1.6, resulted in the production of three‐dimensional thin polyelectrolyte shells upon dissolving the core. The ultrathin shells were observed by means of scanning and transmission electron microscopy. © 1998 John Wiley & Sons, Ltd.

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Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Cited by 249 publications

References 11 publications

Preparation of enzyme multilayers on colloids for biocatalysis

Preparation of enzyme multilayers on colloids for biocatalysis

Evaluation of Encoded Layer‐By‐Layer Coated Microparticles As Protease Sensors

Stepwise polyelectrolyte assembly on particle surfaces: a novel approach to colloid design

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