Sequential activation of two Dof transcription factor gene promoters during vascular development in Arabidopsis thaliana

Konishi, Mineko; Yanagisawa, Shuichi

doi:10.1016/j.plaphy.2007.05.001

Cited by 85 publications

(78 citation statements)

References 24 publications

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“…All genes whose expression has previously been assigned to early stages of vascular development in the leaf have also been reported to be expressed in root vascular cells [e.g., (AlonsoPeral et al, 2006;Baima et al, 1995;Carland and Nelson, 2009;Hardtke and Berleth, 1998;Kang and Dengler, 2004;Konishi and Yanagisawa, 2007;Scarpella et al, 2004;Scarpella et al, 2006;Steinmann et al, 1999;Wenzel et al, 2007)]. Therefore, to identify new preprocambial gene expression profiles, we interrogated an available global gene expression map of the Arabidopsis root (Birnbaum et al, 2003) to extract root vascular-specific gene expression datasets.…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, DOF5.8, which is negligibly expressed in root microarray datasets, displays prominent veinassociated expression (Konishi and Yanagisawa, 2007). Tissuespecific expression patterns are available for 13 of the 36 Arabidopsis DOF genes (Fornara et al, 2009;Gardner et al, 2009;Gualberti et al, 2002;Imaizumi et al, 2005;Konishi and Yanagisawa, 2007;Skirycz et al, 2006Skirycz et al, , 2007Skirycz et al, , 2008Ward et al, 2005). These 13 genes sample the diversity of the DOF family, yet all of them appear to be expressed in vascular strands.…”

Section: Vascular Expression Profiles Of Dof Genesmentioning

confidence: 99%

“…A view of the transcriptional landscape of cells at preprocambial stages would be particularly valuable as it might provide insight into the molecular pathways underlying vein formation. However, very few genes have been identified whose expression is initiated at stages prior to procambium formation [e.g., (Alonso-Peral et al, 2006;Baima et al, 1995;Carland and Nelson, 2009;Hardtke and Berleth, 1998;Kang and Dengler, 2004;Konishi and Yanagisawa, 2007;Scarpella et al, 2004;Steinmann et al, 1999;Wenzel et al, 2007)].…”

Section: Introductionmentioning

confidence: 99%

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Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves

Gardiner

Sherr²,

Scarpella

2010

Int. J. Dev. Biol.

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The sequence of events underlying the formation of vascular networks in the leaf has long fascinated developmental biologists. In Arabidopsis leaves, vascular-precursor procambial cells derive from the elongation of morphologically inconspicuous ground cells that selectively activate expression of the HD-ZIP III gene ATHB8. Inception of ATHB8 expression operationally defines acquisition of a typically irreversible preprocambial cell state that preludes to vein formation. A view of the constellation of genes whose expression is activated at preprocambial stages would therefore be particularly desirable; however, very few preprocambial gene expression profiles have been identified. Here, we show that expression of three genes encoding members of the DOF family of plant-specific transcription factors is activated at stages overlapping onset of ATHB8 expression. Expression of DOF genes is initiated in wide domains that become confined to sites of vein development. Congruence between DOF expression fields and zones of vein formation persists upon experimental manipulation of leaf vascular patterning, suggesting that DOF expression identifies consistently recurring steps in vein ontogeny. Our results contribute to defining preprocambial cell identity at the molecular level.

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Section: Resultsmentioning

confidence: 99%

Section: Vascular Expression Profiles Of Dof Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves

Gardiner

Sherr²,

Scarpella

2010

Int. J. Dev. Biol.

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“…For the construction of binary vectors to generate transgenic Arabidopsis expressing NLP6-VP16, RWPRK-VP80 or NLP6-SUPRD under the control of the 35S promoter, we initially generated a binary vector pCB302-HYG-35S-GUS, which was generated by replacing the bar gene in pCB302-35S-GUS 48 with a hygromycin resistance gene. The GUS gene was then replaced with DNA fragments encoding either NLP6-VP16, RWPRK-VP80 or NLP6-SUPRD.…”

Section: Methodsmentioning

confidence: 99%

“…Transgenic Arabidopsis harbouring the GUS reporter gene under the control of the 8OP promoter was generated by transformation of wild-type Arabidopsis (Columbia) using a binary vector where the 35S promoter in pCB302-HYG-35S-GUS was replaced with the 8OP promoter. Transgenic Arabidopsis lines expressing LexA fusions were produced by retransformation of the transgenic Arabidopsis plants with binary vectors for the expression of LexA-MYC, LexA-VP16, 6N-LexA-MYC or 6N-LexA-6C under the control of the 35S promoter, which were constructed by replacement of the GUS gene in pCB302-35S-GUS 48 with DNA fragments encoding either LexA-MYC, LexA-VP16, 6N-LexA-MYC or 6N-LexA-6C.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

2013

Self Cite

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In plants, nitrate is not only a major nitrogen source but also a signalling molecule that modulates the expression of a wide range of genes and that regulates growth and development. The critical role of nitrate as a signalling molecule has been established for several decades. However, the molecular mechanisms underlying the nitrate response have remained elusive, as the transcription factor that primarily responds to nitrate signals has not yet been identified. Here we show that Arabidopsis NIN-LIKE PROTEIN (NLP) family proteins bind the nitrate-responsive cis-element and activate nitrate-responsive cis-element-dependent and nitrate-responsive transcription. Our results also suggest that the activity of NLPs is posttranslationally modulated by nitrate signalling. Furthermore, the suppression of NLP function impairs the nitrate-inducible expression of a number of genes and causes severe growth inhibition. These results indicate that NLPs are the transcription factors mediating the nitrate signal and thereby function as master regulators of the nitrate response.

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Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development

Gardiner

Donner

Scarpella

2010

Developmental Dynamics

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The processes underlying the formation of leaf vascular networks have long captured the attention of developmental biologists, especially because files of elongated vascular-precursor procambial cells seem to differentiate from apparently equivalent, isodiametric ground cells. In Arabidopsis leaves, ground cells that have been specified to vascular fate engage expression of ARABIDOPSIS THALIANA HOMEOBOX8 (ATHB8). While definition of the transcriptional state of ATHB8-expressing ground cells would be particularly informative, no other genes have been identified whose expression is initiated at this stage. Here we show that expression of SHORT-ROOT (SHR) is activated simultaneously with that of ATHB8 in leaf development. Congruence between SHR and ATHB8 expression domains persists under conditions of manipulated vein patterning, suggesting that inception of expression of SHR and ATHB8 identifies transition to a preprocambial cell state that presages vein formation. Our observations further characterize the molecular identity of cells at anatomically inconspicuous stages of leaf vein development. Developmental Dynamics 240:261-270,

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Sequential activation of two Dof transcription factor gene promoters during vascular development in Arabidopsis thaliana

Cited by 85 publications

References 24 publications

Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves

Expression of DOF genes identifies early stages of vascular development in Arabidopsis leaves

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development

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