Sequential embolization of the branches of the bile duct and portal vein to the targeted hepatic lobe in rats

Shrestha, Anuj; Zhou, Yong; Mao, Hui; Li, Fuyu; Ma, Wen‐Jie; Cheng, Nan‐Sheng; Xu, Ran; Zhang, Yong-Qiong; Jiang, Ting; Feng, Huan; Li, Wen; Han, Qiang

doi:10.1016/s1499-3872(14)60007-2

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Studies have shown that liver resection can cause more serious liver damage than portal ligation 30 , 31 . Compared with the intrahepatic vascular system, bile duct ligation in a specific hepatic lobe can avoid massive hemorrhage caused by vascular surgical injury, which may be safer and more accurate 32 . Therefore, the animal model established in this study is a safe and effective animal model for the study of liver hyperplasia and does not cause serious damage to liver function to avoid impact on liver hyperplasia.…”

Section: Discussionmentioning

confidence: 99%

A modified animal model of hepatic regeneration induced by hilar bile duct ligation

Chai

Chang

et al. 2021

Sci Rep

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Mechanisms of the proliferation of liver are mainly studied in animal model of liver regeneration after partial hepatectomy (PH). However, the PH model involves complex regeneration mechanisms, including hemodynamic factors, cytokines, growth factors, and metabolites. Among liver metabolites, bile acid (BA) is a key signaling molecule that regulates liver regeneration. This study aimed to establish a new type of rapid liver hyperplasia model induced mainly by bile acid pathway through hepatoenteral circulation with hilar bile duct ligation (HBDL). We first established the HBDL model by ligating the bile duct of all hepatic lobes but the right lateral lobe in rabbits and compared with the PVL model and sham operation group. Changes in the liver lobe and hemodynamics were observed. Liver function and the bile acid level were also analyzed. Then we verified the HBDL model in mice. Liver function and the levels of bile acids and cytokines were tested. The protein and mRNA levels of FXR, FGF15, CYP7A1 and FoxM1b in liver tissue were also analyzed. After hilar ligation of the biliary tract, the unligated liver lobes proliferated significantly. Compared with those in the sham group, the volume and weight of the unligated right lateral lobe of the liver in the HBDL group and the PVL group increased significantly (P < 0.05). Transient liver function impairment occurred both in the HBDL group and PVL group in the rabbit model as well as the mouse models. The bile acid levels in the HBDL groups of the rabbit model and mouse model increased significantly within first week after surgery (P < 0.05). The immunohistochemistry results confirmed the proliferation of hepatocytes in the unligated liver lobe. Compared with those in the sham group, the levels of FXR, FGF15 and FoxM1b in the HBDL group were significantly increased (P < 0.05), while the expression of CYP7A1 was inhibited. Compared with those in the HBDL group, the postoperative hemodynamic changes in the PVL group were significant (P < 0.05). The levels of IL-6 and TNF-α in the HBDL group were higher than those in the sham group. The HBDL model is simple to establish and exhibits good surgical tolerance. The model has definite proliferative effect and strong specificity of bile acid pathway. This is an ideal animal model to study the mechanism of liver regeneration through bile acid pathway.

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Section: Discussionmentioning

confidence: 99%

A modified animal model of hepatic regeneration induced by hilar bile duct ligation

Chai

Chang

et al. 2021

Sci Rep

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“…Estos modelos se han utilizado para estudiar los tipos de materiales empleados para la embolización (permanentes o reabsorbibles) [101,107], el efecto que produce en la regeneración hepática la oclusión combinada de venas [108], arterias [109] o conductos biliares hepáticos [110], la tasa de crecimiento tumoral tras PVE [111,112] y la comparación entre diferentes técnicas (PVE vs. PVL [95,100] y, recientemente PVE vs. RE [113] (como detallamos en el apartado siguiente). Del mismo modo, estos modelos se emplean para experimentar con los fármacos que potencialmente mejoran o modulan la regeneración hepática.…”

Section: Modelos Animales De Embolización De Vena Portaunclassified

Desarrollo de un modelo animal de atrofia-hipertrofia y daño hepático tras radioembolización lobar con YTRIO-90 en conejos

Páramo¹

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Background: The study was aimed at: (1) describing the incidence of anatomic variations of the portal system in the rabbit using direct portography; and (2) estimating the liver volume and caudate lobe volume by using contrast-enhanced computed tomography (CECT) in the same animal model. Methods: Forty-six New Zealand white rabbits were included. All of them underwent direct portography and unenhanced CECT. Conventional liver rabbit portal system anatomy (type 1) consisted of the bifurcation of the main portal vein (MPV) into the right portal vein (RPV) and left portal vein (LPV), which subsequently divided into medial left portal vein and lateral left portal vein. Trifurcation of the LPV was considered type 2. The LPV that divides into four smaller branches was classified as type 3. Other configurations of the portal system, including particular cases of MPV branching, were grouped as type 4. Liver lobes were manually segmented. Results: The incidence of each type of portal system anatomy was: type 1, 67.4%; type 2, 15.2%; type 3, 13.0%); and type 4, 4.3%. The mean volume of the caudate lobe was 19.1 ml ± 5.7 ml and of the cranial lobes it was 66. 7 ml ± 13.7 ml, and the total liver volume was 85.7 ml ± 16.7 ml. Conclusions: In New Zealand white rabbits, type 1 is the prevalent type of portal system, liver volume is about 86 ml, and the caudate and cranial lobes are separated. This information could be important when planning experimental rabbit liver procedures.

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Comparable liver function and volume increase after portal vein embolization in rabbits and humans

Olthof

Heger

Lienden

et al. 2017

Surgery

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Sequential embolization of the branches of the bile duct and portal vein to the targeted hepatic lobe in rats

Cited by 3 publications

References 16 publications

A modified animal model of hepatic regeneration induced by hilar bile duct ligation

A modified animal model of hepatic regeneration induced by hilar bile duct ligation

Desarrollo de un modelo animal de atrofia-hipertrofia y daño hepático tras radioembolización lobar con YTRIO-90 en conejos

Comparable liver function and volume increase after portal vein embolization in rabbits and humans

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