2006
DOI: 10.1002/cncr.21905
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Sequential gene expression changes in cancer cell lines after treatment with the demethylation agent 5‐Aza‐2′‐deoxycytidine

Abstract: Pristine‐, poly(octafluorotoluene)‐ (POFT), and polyacetylene (PAc)‐coated Si wafers were used as substrates for the study of segregation of silicone diacrylate (SA) from a formulation containing other oligomeric and monomeric acrylates. POFT and PAc were microwave plasma polymerized on the Si wafers. Three SAs with molecular weights ranging from 700 to 6000 Da were synthesized and characterized. Formulations with 2 wt % SA were ultraviolet cured on the silicon wafers. The surface composition of formulation‐su… Show more

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Cited by 25 publications
(23 citation statements)
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“…34 Overall, gene expression profiles were similar, but there were differences in the expression of several genes between HepG2 and Huh7 cells. 35 Our findings suggest that the effect of AgNPs on metabolism may vary according to cell type, and thus further study is necessary to determine the effect of AgNPs on metabolism in the different types of cells. In our study, PEPCK mRNA levels were not altered, but Nrf2 level was reduced in hepatoma cells 2 hours after AgNP treatment.…”
Section: Discussionmentioning
confidence: 84%
“…34 Overall, gene expression profiles were similar, but there were differences in the expression of several genes between HepG2 and Huh7 cells. 35 Our findings suggest that the effect of AgNPs on metabolism may vary according to cell type, and thus further study is necessary to determine the effect of AgNPs on metabolism in the different types of cells. In our study, PEPCK mRNA levels were not altered, but Nrf2 level was reduced in hepatoma cells 2 hours after AgNP treatment.…”
Section: Discussionmentioning
confidence: 84%
“…Bisulfite genomic sequencing was used to determine the methylation status of promoter regions. By clustering analysis based on expression patterns, we revealed in our previous report that there were 206 genes upregulated by 5-AzaC in at least one HCC cell line and 80 genes upregulated by 5-AzaC in at least two cell lines [11]. To choose the candidate genes regulated by this epigenetic factor, we performed simple linear regression analysis with the statistical program StatView (SAS, Cary, N.C., USA).…”
Section: Methodsmentioning
confidence: 99%
“…In previous studies, we used the microarray system to define the genes upregulated by 5-AzaC treatment [10] and to define serial changes in gene expression in six hepatoma cell lines induced by transient treatment with 5-AzaC for 24–120 h. Based upon the results of these studies, we selected candidate genes that may be regulated by epigenetic factors [11]. In this study,we assessed the methylation status of CpG islands within the promoters of these twelve genes and examined the status of the aberrantly methylated genes in terms of the difference in methylation between HCC and non-HCC tissues.…”
Section: Introductionmentioning
confidence: 99%
“…For this purpose, DAC and TSA were used as a DNA methylation inhibitor and a histone deacetylase inhibitor, respectively. 25,26) The mRNA expression of SMCT1, THTR2 and MATE1 was induced by DAC treatment in HCT116 and SW48 cells lacking their expressions. In addition, in HCT116 and SW48 cells, methylated DNA in the promoter region of THTR2, SMCT1 and MATE1 decreased by DAC treatment, while unmethylated DNA increased.…”
Section: Fig 2 Msp Analysis Of the Upstream Region Of Tss In Colon mentioning
confidence: 99%