Sequential multiple‐scan monitoring using an ion‐trap mass spectrometer

Creaser, Colin S.; Mitchell, D. S.; O’Neill, Kim; Trier, K. J.

doi:10.1002/rcm.1290040610

Cited by 10 publications

(4 citation statements)

References 5 publications

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“…have been described in the literature (56,57). In this case, period 2 of the scan function is set for low rf amplitude injection (low mass cutoff = m/z 50) and period 3 is set for high rf amplitude injection (low mass cutoff = m/z 150).…”

Section: Methodsmentioning

confidence: 99%

Electrospray ionization of porphyrins using a quadrupole ion trap for mass analysis

Berkel

McLuckey

Glish³

1991

Anal. Chem.

173

116

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The utility of electrospray (ES) Ionization combined with km trap mass spectrometry for the analysis of a wide variety of free-base porphyrins and metalloporphyrlns is examined. A single molecular Ionic species Is observed In most cases for the porphyrins under study. The free bases are detected as the protonated molecules, ( + H)+, the trlvalent metalloporphyrlns as (M -L)+, where L~Is a counteranion, and the divalent metalloporphyrlns as M*+, (M + H)+, and/or (M + Na)+, depending upon both the metal complexed and the composition of the electrospray solvent. This marks the first reported observation of radical cations formed by ES Ionization. Detection of the porphyrins studied by using continuous Infusion required sampling as Ittle as 1 fmol of material, while, with flow Injection analysis, detection of 18 fmol of porphyrin Is demonstrated. ES Ionization Is shown to be well suited for porphyrin molecular weight determination and for the analysis of porphyrin mixtures because no fragment Ions are produced. Fragmentation of porphyrin Ions via collision-induced dissociation (CID) and fragmentation Induced by Injection of Ions Into the trap are both shown to provide structure Information. The degree of fragmentation upon Injection can be varied via adjustment of Ion Injection parameters, either manually or automatically under computer control, providing a nonmassselective means to Induce fragmentation In the Ion trap. The potential utHHy of automated control of Ion Injection parameters to provide both molecular weight and structure Information when an on-line separation method Is used with the ES/lon trap system Is demonstrated.

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Section: Methodsmentioning

confidence: 99%

Electrospray ionization of porphyrins using a quadrupole ion trap for mass analysis

Berkel

McLuckey

Glish³

1991

Anal. Chem.

173

116

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“…Tandem mass spectrometry was carried out following microbore HPLC separation and post-column addition of copper via the T-piece. A multiple scan monitoring routine 25 was used to isolate the [M 2 H + Cu(II)] + ion (where M = peptide) of each peptide in turn for collision activated dissociation (CAD). Electrospray ionization conditions were: spray voltage 7 kV, nebulizing gas 20 (arb) units.…”

Section: Microbore Lc-ms With Post-column Metal Complexationmentioning

confidence: 99%

Nano-electrospray and microbore liquid chromatography-ion trap mass spectrometry studies of copper complexation with MHC restricted peptides

Creaser

Lill

Bonner

et al. 2000

Analyst

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The formation of copper/peptide complex ions by nano-electrospray and microbore HPLC-electrospray mass spectrometry has been investigated for major histocompatibility complex (MHC) class I and class II restricted peptides. Post-column addition of copper(II) acetate following microbore HPLC-MS separation was carried out using a mixing T-piece or via the sheath flow inlet of the electrospray source. Optimal analytical conditions for copper complex ion formation were determined by variation of copper concentration, pH, nebulization gas supply and spray voltage. Tandem mass spectrometry of copper/peptide complex ions provides peptide sequence information and insight into the peptide chelation sites. Copper associated y fragment ions dominate the product ion spectrum for non-histidine containing peptides, but both b and y copper complex ions were observed for the histidine containing MHC class I associated peptide gp70.

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“…The EVE was reopened, the cold trap turned off and acquisition by the mass spectrometer started 30 s later. Data were acquired using either full scanning electron ionization (EI) over the range m/z 50-450, selected ion monitoring EI using the mass ranges m/z 205-220 and m/z 375-415 or multiple scan monitoring (MSM), 31 to scan the EI and methane chemical ionization (CI) spectra alternately.…”

Section: Hplc-gc-ms With On-line Derivatisation Of Stilbene Hormones ...mentioning

confidence: 99%

“…Standards and extracts were analysed by HPLC-GC-MS with on-line silylation, using either EI or multiple scan monitoring (MSM) 31 of alternate EI and positive ion CI mass spectra with methane as reagent gas. For the EI experiments, the full m/z 50-450 mass range or the selected mass ranges m/z 205-220 and 375-415 were scanned.…”

Section: Hplc-gc-ms With On-line Dervatisation Of Stilbene Hormones I...mentioning

confidence: 99%

On-line Derivatisation in Combined High-performance Liquid Chromatography–Gas Chromatography–Mass Spectrometry

Chappell¹,

Creaser²,

Shepherd³

1997

Analyst

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Procedures are reported for the combined HPLC-GC and HPLC-GC-MS analysis of involatile analytes using on-line derivatisation. Derivatisation was carried out between the HPLC and the GC separation stages using two approaches; pre-mixing of the derivatisation reagent and the analytes in the HPLC effluent ahead of the GC pre-column, and independent reagent delivery after deposition of the desolvated analytes in the GC pre-column. The derivatisation of the stilbene hormones, quinoxaline-2-carboxylic acid, sulfamethazine and 2-naphthoic acid with silylation, methylation and acetylation reagents was investigated. Derivatisation efficiencies in the range 84-100% were achieved for the stilbene hormones and acidic compounds. The HPLC-derivatisation-GC procedure was demonstrated for the determination of the stilbene hormones in processed meats with detection limits between 0.17 and 0.47 mg kg 21 and RSDs in the range 2.3-4.9% for HPLC-GC with electron ionization quadrupole ion trap MS detection.

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Sequential multiple‐scan monitoring using an ion‐trap mass spectrometer

Cited by 10 publications

References 5 publications

Electrospray ionization of porphyrins using a quadrupole ion trap for mass analysis

Electrospray ionization of porphyrins using a quadrupole ion trap for mass analysis

Nano-electrospray and microbore liquid chromatography-ion trap mass spectrometry studies of copper complexation with MHC restricted peptides

On-line Derivatisation in Combined High-performance Liquid Chromatography–Gas Chromatography–Mass Spectrometry

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