Sequential pretreatment of sugarcane bagasse by alkali and organosolv for improved delignification and cellulose saccharification by chimera and cellobiohydrolase for bioethanol production

Production of Sugar from Cassava Peel using Different Chemical Pre-treatment

“…This finding was similar to [69], who found that delignification increased with the increase of ethanol concentration as an organic solvent in organosolv pre-treatment. This is similar to previous research that reported increases in delignification cause higher cellulose exposure and cellulose conversion to glucose [70]- [72]. On the contrary, [3], [60] found no trend in the glucose yield under different organosolv concentrations.…”

Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses

“…6 b). The TRS yield generated by RfGH5_4 from alkali pretreated SDR and SBG was significantly higher than the earlier reported TRS yields for these biomasses (34.2 mg/g for SDR, [27] and 5.9 mg/g for SBG, [54]. Jamaldheen et al [27] used endoglucanase (CtCel8A, 80 U/mg) and β-glucosidase (CtBgl1A, 33 U/mg) of C. thermocellum for SDR saccharification.…”

“…Jamaldheen et al [27] used endoglucanase (CtCel8A, 80 U/mg) and β-glucosidase (CtBgl1A, 33 U/mg) of C. thermocellum for SDR saccharification. Nath et al [54] employed chimera, CtGH1-L1-CtGH5-F194A (240 U/g) containing β-glucosidase and endoglucanase along with cellobiohydrolase, CtCBH5A (360 U/g) from C. thermocellum to saccharify SBG. However, the TRS yield from RfGH5_4 saccharified CMS and CSB was comparatively lower than the reported value (490 mg/g) in a previous report [25], where the alkali pre-treated 5% (w/v) cotton stalk was saccharified by 100 U of commercial cellulase enzyme mixture at 50 • C for 72 h. The TRS obtained in the current study could be lower because, a lower biomass (pre-treated CMS or CSB) loading, 2% (w/v) was saccharified and for shorter period (48 h) using an in house produced single enzyme, endoglucanase (RfGH5_4) at 30 • C which is a costeffective process having lesser energy requirements.…”