Sequential resolution of (<i>S</i>) and (<i>R</i>)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn

Jiang, Min; Liu, Yongjun; Liu, Guosong; Zhou, Wei; Shen, Ya ling; Gao, Bei; Wang, Fengqing; Wei, Dongzhi

doi:10.1039/d1gc04512a

Cited by 1 publication

(2 citation statements)

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“…388 Various biocatalytic methods for the synthesis of carboxylic acids have been developed, complementing nonenzymatic approaches. 389 These methods include the hydrolysis of nitriles by nitrilases, 390−392 the conversion of aldehydes by hydroxynitrile lyases with subsequent hydrolysis, 393 the hydrolysis of esters by esterases, 394 and the oxidation of alcohols or aldehydes by dehydrogenases and oxidases. 395 Glieder et al reported a hydroxynitrile lyase mutant from Prunus amygdalus for the enantioselective synthesis of α-hydroxyacids by the addition of HCN to aldehydes and the subsequent hydrolysis of the intermediately formed cyanohydrins.…”

Section: Chromo-and Fluorogenic Probes For Carboxylic Acidsmentioning

confidence: 99%

“…Various biocatalytic methods for the synthesis of carboxylic acids have been developed, complementing nonenzymatic approaches . These methods include the hydrolysis of nitriles by nitrilases, − the conversion of aldehydes by hydroxynitrile lyases with subsequent hydrolysis, the hydrolysis of esters by esterases, and the oxidation of alcohols or aldehydes by dehydrogenases and oxidases …”

Section: Chromo- and Fluorogenic Probes For Carboxylic Acidsmentioning

confidence: 99%

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Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to their high speed and sensitivity. A large proportion of studies relies on fluorogenic substrates that mimic the chemical properties of the target or coupled enzymatic assays with an optical read-out that assesses the desired catalytic efficiency indirectly. The most reliable hits, however, are achieved by screening for conversions of the starting material to the desired product. For this purpose, functional group assays offer a general approach to achieve a fast, optical read-out. They use the chemoselectivity, differences in electronic and steric properties of various functional groups, to reduce the number of false-positive results and the analytical noise stemming from enzymatic background activities. This review summarizes the developments and use of functional group probes for chemoselective derivatizations, with a clear focus on screening for enzymatic activity in protein engineering.

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Section: Chromo-and Fluorogenic Probes For Carboxylic Acidsmentioning

confidence: 99%

Section: Chromo- and Fluorogenic Probes For Carboxylic Acidsmentioning

confidence: 99%

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn

Abstract: Optically pure 6-fluoro-chroman-2-carboxylic acids (FCCAs), including (S) and (R)- configuration, are pivotal chiral building blocks in the pharmaceutical industry. To date, the production of (S) and (R)-FCCA mainly depends on...

Cited by 1 publication

References 26 publications

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

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