Sequential solid-state and submerged cultivation of Aspergillus niger on sugarcane bagasse for the production of cellulase

Cunha, F. M.; Esperança, Mateus N.; Zangirolami, Teresa Cristina; Badino, Alberto Colli; Farinas, Cristiane S.

doi:10.1016/j.biortech.2012.02.082

Cited by 126 publications

(61 citation statements)

References 29 publications

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“…The unconventional agitated pre-culture fermentation method (agitated-SmF) therefore had a positive influence on lignocellulolytic enzyme production, and similar results have been found for filamentous fungal morphological characteristics in submerged cultivations, which had an effect on cellulase production (Domingues et al 2000;Cunha et al 2012). Hence, this is the first work reporting on basidiomycetes using this pre-culture fermentation method and it has provided promising results.…”

Section: Effect Of Fermentation Methods On Production Of Lignocellulolsupporting

confidence: 66%

“…Lignocellulolytic enzymes are currently produced by using either solid-state fermentation (SSF) or submerged fermentation (SmF) (Kang et al 2004;Singhania et al 2010;Farinas et al 2011). However, it seems that there have been few reports on lignocellulolytic enzyme production in submerged fermentation after a solid-state pre-culture step in fungi (Cunha et al 2012), and particularly no report in white rot fungi. There also have been no studies showing comparisons of lignocellulolytic enzyme production using various fermentation methods, including sequential solid-state and submerged fermentation, and conventional solid-state fermentation and submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

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Sequential Solid-State and Submerged Cultivation of the White Rot Fungus Pleurotus ostreatus on Biomass and the Activity of Lignocellulolytic Enzymes

An¹,

Wu²,

Han³

et al. 2016

BioResources

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Sequential solid-state and submerged cultivation with various lignocellulosic biomasses as a substrate for lignocellulolytic enzyme production by Pleurotus ostreatus were assessed by measuring endoglucanase, xylanase, and laccase activities. An unconventional preculture method was established by cultivating the P. ostreatus mycelia in a solid substrate medium for an initial fungal growth phase, followed by a transition to submerged fermentation through adding a liquid culture medium. The lignocellulolytic enzymes of P. ostreatus in different fermentation methods revealed wide differences. The higher yields of endoglucanase (3152 ± 139 U/L), xylanase (3064 ± 40 U/L), and laccase (543 ± 21 U/L) were achieved by using the sequential solid-state submerged method compared to conventional solid-state and submerged cultivation. Generally speaking, sequential solid-state and submerged fermentation of cottonseed hull is favorable for laccase secretion, whereas sequential solid-state and submerged fermentation of corncob provides better production of hydrolytic enzymes. These results revealed that the nature of the lignocellulosic biomass and the fermentation method play an important role in the expression of lignocellulolytic enzymes. This indication would be helpful in optimizing the production of integrated industrial lignocellulolytic enzymes.

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Section: Effect Of Fermentation Methods On Production Of Lignocellulolsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Sequential Solid-State and Submerged Cultivation of the White Rot Fungus Pleurotus ostreatus on Biomass and the Activity of Lignocellulolytic Enzymes

An¹,

Wu²,

Han³

et al. 2016

BioResources

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“…Previous research reported that Aspergillus niger was produced 0.71 U/mL of xylanase activities after 30 hours' incubation times [17]. Comparison of seed culture conditions using different subculture of Pycnoporus sanguineus Based on the findings, comparisons between both subcultures were analyzed.…”

Section: Xylanase Enzymes Activitiesmentioning

confidence: 99%

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus

Jalil

Kalil²,

Rahman³

et al. 2017

MJAS

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Low yields and productivity in fermentation industry usually due to poor quality of seed culture (inoculum). As the first stage in the fermentation process, inoculum consistency in terms of size and quality is clearly important for high yield of enzymes production. Selection of microorganism also plays an important role in improving the productivity. Fungi type of white rot basidiomycetes species is a well-known cellulolytic enzymes producer and capable to degrade many types of lignocellulosic biomass. Cellulolytic enzymes produced from white rot fungi, Pycnoporus sanguineus (PS) were investigated. The PS cultured on different agar media and parameter conditions of seed culture preparation in liquid medium broth were compared. Seed culture conditions of PS were influenced by many factors such as type of medium used for fungal growth, temperature, incubation time in liquid medium and subculture time on agar medium. PS was full-grown on potato dextrose agar (PDA) on day 5 compared to malt extract agar (MEA) on day 7. Seed culture conditions was determined using standard liquid medium, potato dextrose broth (PDB) at constant temperature (30 °C ), agitation speed (150 rpm) and pH (4.8) for duration of seven days using two different subculture of PS that were grown for five and seven days. It was found that the five days' subculture of PS shown 3.93% higher cell dry weight of fungal biomass (3.44 g/L on day 5) and higher cellulolytic enzymes activity; 95.49% (FPase), 3.14% (CMCase), 7.71% (exoglucanases) and 14.93% (xylanases). Maximum cellulolytic enzymes were found after 48 hours' incubation with filter paper activity (FPase) of 1.79 U/mL, carboxymethyl cellulase activity (CMCase) of 3.36 U/mL, exoglucanase activity (Avicelase) of 0.59 U/mL and xylanase enzymes activity of 0.66 U/mL. Reducing sugars concentration decreased from day 1 to 7 due to consumption of sugars for fungal growth. Seed culture conditions were strongly influenced by subculture and incubation time in liquid medium to produce high yield of cellulolytic enzymes from PS.Keywords: white rot fungi, Pycnoporus sanguineus, seed culture, cellulolytic enzymes, fermentation Abstrak Hasil dan produktiviti yang rendah dalam industri fermentasi biasanya disebabkan oleh kultur benih (inokulum) yang kurang berkualiti. Pada peringkat pertama proses fermentasi, konsistensi inokulum dari segi saiz dan kualiti sangat penting bagi pengeluaran enzim yang tinggi. Pemilihan mikroorganisma juga memainkan peranan penting dalam meningkatkan produktiviti. Jenis kulat pereput putih dari spesies basidiomycetes sangat terkenal sebagai pengeluar enzim selulolitik dan ia mampu untuk mengurai pelbagai jenis bahan lignoselulosa. Dalam kajian ini, enzim selulolitik dihasilkan daripada kulat pereput putih, Pycnoporus sanguineus (PS) yang dihidupkan di atas agar dan perbandingan parameter berbeza penyediaan kultur benih dalam medium cecair dikaji. Kultur benih PS dipengaruhi oleh pelbagai faktor seperti jenis medium yang digunakan untuk pertumbuhan kulat, suhu, masa pengera...

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“…However, the high hydrolysis costs are still a limiting factor for its industrial application (Cardona et al 2010). Thus, the search for new enzymes might improve the productivity and reduce costs in making the lignocellulose hydrolysis on industrial scale (Cunha et al 2012;Singhvi et al 2011). As regards the search for new industrial enzymes, the use of new strains may be an alternative method to improve the hydrolysis process.…”

Section: Introductionmentioning

confidence: 99%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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Background:The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coconut water consumption. In the present work, a new microbial strain was isolated from the coconut shell powder, molecularly identified as Melanoporia sp. CCT 7736 and applied for cellulase production in solid-state fermentation using the green coconut shell powder as substrate. Results:The complete production process was optimized. Fermentation time was only 24 h, and the enzyme produced presented maximal activity at neutral pH (6.5) and 60 °C. The maximal enzyme activity after extraction optimization was 7.5 IU/gds (international units of enzyme activity per gram of dry solid). For the enzyme extraction, the rotation velocity, the extraction time, the temperature, and the solvent volume (buffer) were optimized using response surface methodology (RSM). The best results for the enzyme extraction were obtained at 250 rpm (orbital shaker) at 30 °C using 13.79 mL of a sodium acetate buffer (200 mM) at pH 6.5 after 10 min. Delignification pretreatment was not necessary since this fungus strain was able to degrade the lignin. Conclusions:To the best of our knowledge, this work is the first report of cellulase production by Melanoporia sp. CCT 7736. Good results were obtained without the need for expensive pretreatment usually applied to lignocellulosic residues because the strain was isolated from coconut shell powder, and it is well adapted to this kind of substrate. The enzyme presented maximum activity at neutral pH instead of acidic pH as reported for the majority of industrial cellulases. The use of lignified coconut shell and the optimal pH at neutral values are the main advantages of the enzyme produced by Melanoporia sp. CCT 7736. In addition, the enzyme showed good stability during storage even at the crude broth and without any cryoprotection.

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Sequential solid-state and submerged cultivation of Aspergillus niger on sugarcane bagasse for the production of cellulase

Cited by 126 publications

References 29 publications

Sequential Solid-State and Submerged Cultivation of the White Rot Fungus Pleurotus ostreatus on Biomass and the Activity of Lignocellulolytic Enzymes

Sequential Solid-State and Submerged Cultivation of the White Rot Fungus Pleurotus ostreatus on Biomass and the Activity of Lignocellulolytic Enzymes

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

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