Sequential Statistical Improvement of the Liquid Cultivation of <i>Helicobacter pylori</i>

Duque-Jamaica, Rocio; Arévalo-Galvis, Azucena; Poutou-Piñales, Raúl A.; Trespalacios, Alba Alicia

doi:10.1111/j.1523-5378.2010.00763.x

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…Recently, Duque-Jamaica et al (35) have also used the BoxBehnken design to optimize some culture conditions (shaking speed, inoculum size and volume of culture media) and the concentration of some components (lamb-blood, DENT and Vitox) of a complex medium to increase the planktonic H. pylori cell culture. The authors carried out the experiments in a mini-bioreactor that supplied CO 2 directly to the culture medium and they were able to achieve 25x10 8 cells/ml after 12 h of growth under their previously optimized conditions.…”

Section: Discussionmentioning

confidence: 99%

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Bessa

Correia

Cellini

et al. 2012

Int J Immunopathol Pharmacol

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Helicobacter pylori is a gastroduodenal pathogen that colonizes the human stomach and is the causal agent of gastric diseases. From the clinical and epidemiological point of view, enhancing and improving the growth of this bacterium in liquid media is an important goal to achieve in order to allow the performance of accurate physiological studies. The aim of this work was to optimize three culture conditions that influence the growth of H. pylori in the defined medium Ham's F-12 supplemented with 5% fetal bovine serum by using response surface methodology as a statistical technique to obtain the optimal conditions. The factors studied in this experimental design (Box-Behnken design) were the pH of the medium, the shaking speed (rpm) and the percentage of atmospheric oxygen, in a total of 17 experiments. The biomass specific growth rate (J1) was the response measured. The model was validated for pH and shaking speed. The percentage of atmospheric oxygen did not influence the growth for the range of values studied. At the optimal values found for pH and shaking speed, 8 and 130 rpm, respectively, a specific growth rate value of 0.164 hot, corresponding to a maximal concentration of approximately 1.5xl0 8 CFU/ml, was reached after 8 h. The experimental design strategy allowed, for the first time, the optimization of H. pylori growth in a semi-synthetic medium, which may be important to improve physiological and metabolic studies of this "fastidious" bacterium.

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Section: Discussionmentioning

confidence: 99%

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Bessa

Correia

Cellini

et al. 2012

Int J Immunopathol Pharmacol

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“…Usually, liquid cultures such as Brucella broth (BD), Mueller-Hilton and Brain Heart Infusion (BHI) are supplemented with 2% to 10% calf serum or 0.2% to 1% β-cyclodextrin, antibiotics, amino acids, and vitamins. Fetal bovine serum has been used as culture media supplement in most studies and it remains one of the most influential in vitro growth factors (Duque-Jamaica et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different culture media for detection and quantification of H. pylori in environmental and clinical samples

et al. 2020

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The objective of the present study was to establish the most suitable culture medium for the isolation of H. pylori from environmental and clinical samples.Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated with serial dilutions of H. pylori NCTC 11637 strain at a final concentration of 10 4 and 10 3 CFU/ml and the recovery rates were calculated.From inoculated tap water and wastewater samples, H. pylori colonies were recovered from four out of the analyzed culture media. When fecal samples were analyzed, the isolation of the pathogen under study was only possible from two culture media. Different optimal media were observed for each type of sample, even for wastewater and stool samples. Nevertheless, our results indicated that the combination of Dent Agar with polymyxin B sulfate did not inhibit the growth of H. pylori and was highly selective for its recovery, regardless of the sample origin. Thus, we propose the use of this medium as a diagnostic tool for the isolation of H. pylori from environmental and clinical samples, as well as for epidemiological studies.

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“…To optimize variables with the greatest effect on biomass production, obtained from the Plackett-Burman design, a Box-Behnken Design [14,15] was used. Three variables were included in the design (Glucose, Tween 80 and pH).…”

Section: Optimization Of Carbon Nitrogen and Selected Inducersmentioning

confidence: 99%

Culture Media Statistical Optimization for Biomass Production of a Ligninolytic Fungus for Future Rice Straw Degradation

et al. 2013

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The main objective of this study was to optimize a culture media for low scale biomass production of Pleurotus spp. Future applications of this optimization will be implemented for ''in situ'' rice straw degradation, increase soil nutrients availability, and lower residue and rice culture management costs. Soil samples were taken from different points in six important rice production cities in Colombia. For carbon and nitrogen source selection a factorial 4 2 design was carried out. The Plackett-Burman design permitted to detect carbon, nitrogen and inducer effects on fungus growth (response variable for all designs). This optimization was carried out by a Box-Behnken design. Finally a re-optimization assay for glucose concentration was performed by means of a One Factor design. Only 4/33 (12 %) isolates showed and important laccase or manganese peroxidase activity compared to Pleurotus ostreatus (HPB/P3). We obtained an increased biomass production in Pleurotus spp. (T1.1.) with glucose, followed by rice husk. Rice straw was considered an inducing agent for lignin degradation. Glucose was a significant component with positive effects, whereas Tween 80 and pH had negative effects. On the contrary, rice husk, yeast extract and CaCl 2 were not significant components for increase the biomass production. Final media composition consisted of glucose 25 g L , and pH 4.88 ± 0.2. The Box-Behnken polynomial prediction resulted to be lower than the experimental validation of the model (6.59 vs. 6.91 Log 10 CFU ml -1 respectively).

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Sequential Statistical Improvement of the Liquid Cultivation of Helicobacter pylori

Cited by 7 publications

References 24 publications

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

Evaluation of different culture media for detection and quantification of H. pylori in environmental and clinical samples

Culture Media Statistical Optimization for Biomass Production of a Ligninolytic Fungus for Future Rice Straw Degradation

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