Sequential Thermochemical Hydrolysis of Corncobs and Enzymatic Saccharification of the Whole Slurry Followed by Fermentation of Solubilized Sugars to Ethanol with the Ethanologenic Strain Escherichia coli MS04

Pedraza, Lorena; Flores, Araceli; Toribio, Héctor; Quintero, Rodolfo; Borgne, Sylvie Le; Moss-Acosta, Cessna L.; Martı́nez, Alfredo

doi:10.1007/s12155-016-9756-9

Cited by 25 publications

(16 citation statements)

References 30 publications

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“…The kineticprofiles of saccharification and fermentation using IL and OV pretreated agave bagasse are shown in Figure 2 and Table 1 xylose by E. coli MS04 in the same reactor with no solids removal (where the process temperature was modified accordingly to the enzyme/bacteria requirements)hence improving overall ethanol yields (Pedraza et al, 2016).…”

Section: Effect Of Pretreatment On Sequential Enzymatic Saccharificatmentioning

confidence: 99%

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

Perez-Pimienta

Vargas‐Tah

López-Ortega

et al. 2017

Bioresource Technology

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Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48h), 12.1 and 12.7kg of ethanol were produced per 100kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications.

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Section: Effect Of Pretreatment On Sequential Enzymatic Saccharificatmentioning

confidence: 99%

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

Perez-Pimienta

Vargas‐Tah

López-Ortega

et al. 2017

Bioresource Technology

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“…As observed, the simultaneous consumption of glucose and XGM was determined in the fermentation of both detoxified hydrolysates, although the consumption rate of XGM sugars by E. coli was also higher for the hydrolysate detoxified with activated charcoal. Simultaneous consumption of glucose and XGM by E. coli has also been reported in the fermentation of hemicellulosic hydrolysates from Agave lechuguilla [31], corncobs [33], and rapeseed straw [13]. It is worth mentioning that levels of arabinose identified in the EOP hydrolysates were not relevant (<9% of total sugars).…”

Section: Ethanol Fermentation By E Colimentioning

confidence: 75%

Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

et al. 2020

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Exhausted olive pomace (EOP) is the main residue generated in olive oil industries, after the extraction of the residual oil from olive pomace with hexane. This work studies the ethanol production from hemicellulosic sugars of EOP. The fermentability of the sugar solution, resulting from the acid pretreatment of EOP, was evaluated using Escherichia coli SL100, although a detoxification step was required before fermentation. Overliming and activated charcoal detoxification were tested to minimize the presence of inhibitory compounds in the hydrolysate and to achieve a fermentable medium. E. coli assimilated all sugars in both detoxified hydrolysates and achieved ethanol yields of about 90% of the theoretical one. However, the fermentation time was much shorter when the hydrolysate had been detoxified with activated charcoal (20 h versus 120 h).

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“…In the field of bioenergy, to obtain bioethanol the castor cake was fermented with S. cerevisiae in the synergism with the cellulase (CMCase) of Pseudomonas (Abada et al, 2019). Similarly, maize cob was fermented by Escherichia coli to obtain bioethanol after being hydrolyzed by hydrolase and saccharified by a saccharifying enzyme (Pedraza et al, 2016).…”

Section: Purpose Of Microbe and Enzyme Cooperationmentioning

confidence: 99%

Synergism of microorganisms and enzymes in solid-state fermentation of animal feed. A review

Li¹,

Cheng²,

Zhang³

et al. 2021

J. Anim. Feed Sci.

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Sequential Thermochemical Hydrolysis of Corncobs and Enzymatic Saccharification of the Whole Slurry Followed by Fermentation of Solubilized Sugars to Ethanol with the Ethanologenic Strain Escherichia coli MS04

Cited by 25 publications

References 30 publications

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

Synergism of microorganisms and enzymes in solid-state fermentation of animal feed. A review

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