Sequential Uptake of Horseradish Peroxidase by Lymphoid Follicle Epithelium of Peyer's Patches in the Normal Unobstructed Mouse Intestine: An Ultrastructural Study

Owen, Robert L.

doi:10.1016/s0016-5085(77)80254-0

Cited by 626 publications

(158 citation statements)

References 44 publications

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“…The microvilli actually are replaced by microfolds, hence the name "M cell." In addition, M cells have increased numbers of cytoplasmic vesicles in which particulate antigen is transported across the cell from luminal to basement membrane surface (Owen and Jones, 1974;Owen, 1977;Smith and Peacock, 1992). Some viruses and bacteria have been shown to bind to M cells specifically (Inman and Cantey, 1971;Carter and Collins, 1974;Wolf er ai, 1981), making this a preferential route of entry.…”

Section: Antigen Uptake and Presentationmentioning

confidence: 99%

“…The absorptive epithelial cells on the luminal two-thirds of the villi of the small bowel of rat and human express the major histocompatibility complex (MHC) Class II antigen (Scott et al, 1980;Mayrhofer et al, 1983). The FAE overlying the Peyer's patches, on the other hand, are devoid of MHC Class II expression (Owen, 1977), suggesting that the intestinal epithelial cells may have a role in antigen presentation distinct from that of the FAE. Interestingly, the epithelial cells have been shown to stimulate the CD8 or cytotoxic/ suppressor T cell preferentially (Mayer and Schlien, 1987;Bland, 1988;Bland and Kambarage, 1991).…”

Section: Absorptive Epithelial Cellsmentioning

confidence: 99%

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Characteristics and Functions of Mucosa-Associated Lymphoid Tissue

Croitoru¹,

Bienenstock²

1994

Handbook of Mucosal Immunology

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Section: Antigen Uptake and Presentationmentioning

confidence: 99%

Section: Absorptive Epithelial Cellsmentioning

confidence: 99%

Characteristics and Functions of Mucosa-Associated Lymphoid Tissue

Croitoru¹,

Bienenstock²

1994

Handbook of Mucosal Immunology

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“…The small intestine, particularly its Peyer's patch region, is capable of assimilating antigenically significant amounts of macromolecules (1,2). This study was designed to determine whether normal patterns of macromolecular absorption are distorted by mucosal disease.…”

mentioning

confidence: 99%

Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet

Keljo¹,

Butler²,

Hamilton³

1985

Gastroenterology

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We studied the macromolecular permeability of segments of jejunum from 2-wk-old piglets after the animals had been experimentally infected with an invasive enteric virus , transmissible gastroenteritis virus. Jejunal segments were mounted in Ussing chambers at stages of the infection, and permeability was measured using three probe molecules of differing molecular weights. In control tissue, permeability to horseradish peroxidase was 2.6 times higher across segments with Peyer's patches than across segments without Peyer's patches, whereas polyethylene glycol 4000 and mannitol permeabilities were the same in patch and nonpatch segments. Twelve hours after infection, when virus had invaded the mucosa causing a structural lesion, and before diarrhea had begun, horseradish peroxidase permeability increased in non-patch-containing segments to equal that across patch-containing tissue. At this early 12-h stage, polyethylene glycol 4000 and mannitol permeation were unchanged in patchcontaining segments compared with controls. Ninety-six hours after transmissible gastroenteritis infection, when diarrhea was severe, horseradish peroxidase permeability in patch-free segments had

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“…Selection of antigens is generally thought to be nonspecific. Substances retained by M cells include ferritin [1], horseradish peroxidase [22], and carbon particles [13]. Reoviruses, known to penetrate into the intestinal epithelium via the M cells [31] did not interact with M cells specifically [30].…”

Section: Discussionmentioning

confidence: 99%

Coxsackievirus-cell interactions that initiate infection in porcine ileal explants

Heinz

Cliver

1988

Archives of Virology

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Coxsackievirus B5 (CB5) labeled with tritiated uridine was used to trace the interaction of the virus with explant cultures of porcine ileum. Similarly labeled human poliovirus 1 (PO 1), which is not specifically retained by porcine tissue, was used as a control. The explant procedure employed could maintain ileal tissue in a differentiated state for up to 48 hours. Porcine ileum was acquired from both young (4-6 week-old) and adult (9-11 month-old) animals. Inoculated explants of either absorptive or lymphoid tissue were incubated at temperatures selected to permit either viral adsorption or penetration and elution to occur. Retention of radioactive virus was quantitated by liquid scintillation counting and localized by autoradiography. Only in absorptive tissue explants from young animals did adsorption of CB5 at 6 degrees C exceed penetration at 37 degrees C. This suggested that incubation at 6 degrees C may not be an appropriate condition for studying enterovirus adsorption in explants. CB5 penetrated most efficiently into lymphoid tissue explants from young animals, indicating that these tissues could discriminate between CB5 and PO 1. In explants from adults, CB5 penetrated equally well into lymphoid and absorptive tissues. Virus penetrated into the absorptive epithelial cells and, possibly, the lamina propria near the villous tips. Low efficiency of penetration, and the non-critical function of these target cells, may help account for the characteristic lack of gastrointestinal symptoms in enterovirus infections.

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Sequential Uptake of Horseradish Peroxidase by Lymphoid Follicle Epithelium of Peyer's Patches in the Normal Unobstructed Mouse Intestine: An Ultrastructural Study

Cited by 626 publications

References 44 publications

Characteristics and Functions of Mucosa-Associated Lymphoid Tissue

Characteristics and Functions of Mucosa-Associated Lymphoid Tissue

Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet

Coxsackievirus-cell interactions that initiate infection in porcine ileal explants

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