Sequestration of human cytomegalovirus by human renal and mammary epithelial cells

Andrei, Gracíela; Kummert, Caroline; Donner, Cathérine; Pérez‐Morga, David; Vos, Rita De; Snoeck, Robert; Marchant, Arnaud

doi:10.1016/j.virol.2014.04.032

Cited by 15 publications

(11 citation statements)

References 59 publications

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“…EBV detection in breast milk may also be associated with MTCT of HIV-1, but only marginally so. CMV DNA may be cellassociated in a variety of infected breast milk cells, including mammary epithelial cells, monocytes and T cells that traffic into breast milk [4,34]. Similar to previous studies, CMV DNA was detectable in the majority of breast milk samples studied from this population of untreated HIV-1-infected mothers, with measured CMV levels significantly higher in breast milk than levels previously reported in blood.…”

Section: Discussionsupporting

confidence: 86%

Cytomegalovirus, and possibly Epstein–Barr virus, shedding in breast milk is associated with HIV-1 transmission by breastfeeding

Viljoen

Tuaillon

Nagot

et al. 2015

Aids

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Section: Discussionsupporting

confidence: 86%

Cytomegalovirus, and possibly Epstein–Barr virus, shedding in breast milk is associated with HIV-1 transmission by breastfeeding

Viljoen

Tuaillon

Nagot

et al. 2015

Aids

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“…In addition, HCMV infection can inhibit the production of erythropoietin and induce or exacerbate anemia [26]. It has been reported that human cytomegalovirus can sequestrate in human renal and mammary epithelial cells [27] and induce TGF-β1 activation in renal tubular epithelial cells after epithelial-tomesenchymal transition [28]. However, it is unclear whether these immunological abnormalities, hematological abnormalities, and renal manifestations were thought to be due to HCMV infection itself or lupus activated through HCMV infection and need further investigation.…”

Section: Discussionmentioning

confidence: 99%

Correlation between systemic lupus erythematosus and cytomegalovirus infection detected by different methods

et al. 2015

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Human cytomegalovirus (HCMV), a β-herpes virus subfamily member, leads to a lifelong, latent infection in most humans, but the correlation between HCMV infection and systemic lupus erythematosus (SLE) remains controversial. We analyzed the relevance of HCMV infection in SLE by analyzing the peripheral blood leukocytes (PBLs) and serum samples of 60 patients with SLE and 111 healthy individuals. HCMV genes UL55 and UL138 were detected in PBLs by polymerase chain reaction (PCR), and HCMV-specific serum IgG and IgM antibodies were investigated by enzyme-linked immunosorbent assay. The relationship between cellular HCMV infection in PBLs and common clinical indicators of SLE was further explored. Data indicated that the frequency of positive IgG and IgM anti-CMV antibodies was not significantly different in SLE patients and controls. However, compared to the healthy controls, the titers of IgG and IgM anti-CMV antibodies in SLE patients were significantly higher. The detection of cellular HCMV infection showed that almost all subjects were positive for UL138 gene in PBLs, but the positivity for UL55 gene was lower in PBLs. HCMV UL138 detection in PBLs was highly consistent with the frequency of the HCMV-specific IgG test and did not show significant difference in SLE patients and healthy controls. However, compared with that in healthy people, the positivity rate for cellular HCMV UL55 detection was significantly higher in SLE patients (P < 0.001). In addition, cellular HCMV UL55 with positive detection in PBLs was associated with significantly different clinical characteristics of SLE than that with negative detection. In conclusion, our data confirmed that the HCMV infection was related to the development of SLE. Especially, some clinical strains or substrains of HCMV, such as containing the UL55 gene in HCMV's genome, might play a vital role in the development of SLE.

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“…Surprisingly, HCMV infection in other types of epithelial cells has received far less study. Sporadic reports of in vitro HCMV infections of epithelial cells from the cervix (13), cochlea (18), kidney (19), mammary gland (20), and thyroid (21) have appeared. These infections were found to be productive.…”

Section: Figmentioning

confidence: 99%

Human Cytomegalovirus Productively Replicates In Vitro in Undifferentiated Oral Epithelial Cells

Weng

Lee

Gelbmann

et al. 2018

J Virol

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Human cytomegalovirus (HCMV) productive replication is most often studied in fibroblasts., fibroblasts amplify viral titers, but transmission and pathogenesis require the infection of other cell types, most notably epithelial cells. , the study of HCMV infection of epithelial cells has been almost exclusively restricted to ocular epithelial cells. Here we present oral epithelial cells with relevance for viral interhost transmission as an model system to study HCMV infection. We discovered that HCMV productively replicates in normal oral keratinocytes (NOKs) and telomerase-immortalized gingival cells (hGETs). Our work introduces oral epithelial cells for the study of HCMV productive infection, drug screening, and vaccine development. The ocular epithelial cells currently used to study HCMV infections have historical significance based upon their role in retinitis, an HCMV disease most often seen in AIDS patients. However, with the successful implementation of highly active antiretroviral therapy (HAART) regimens, the incidence of HCMV retinitis has rapidly declined, and therefore, the relevance of studying ocular epithelial cell HCMV infection has decreased as well. Our introduction here of oral epithelial cells provides two alternative models for the study of HCMV infection that complement and extend the physiologic relevance of the ocular system currently in use.

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Sequestration of human cytomegalovirus by human renal and mammary epithelial cells

Cited by 15 publications

References 59 publications

Cytomegalovirus, and possibly Epstein–Barr virus, shedding in breast milk is associated with HIV-1 transmission by breastfeeding

Cytomegalovirus, and possibly Epstein–Barr virus, shedding in breast milk is associated with HIV-1 transmission by breastfeeding

Correlation between systemic lupus erythematosus and cytomegalovirus infection detected by different methods

Human Cytomegalovirus Productively Replicates In Vitro in Undifferentiated Oral Epithelial Cells

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