Serial lectin affinity chromatography with concanavalin A and wheat germ agglutinin demonstrates altered asparagine-linked sugar-chain structures of prostatic acid phosphatase in human prostate carcinoma

Tumor Biomarker Glycoproteins in the Seminal Plasma of Healthy Human Males Are Endogenous Ligands for DC-SIGN

“…Clusterin isolated from human serum bears a total of six occupied N-glycosylation sites associated with its disulfide-linked ␣-chain (Asn 64 (69,70). In summary, all of the major endogenous glycoprotein ligands for DC-SIGN detected in human seminal plasma express multiple N-glycosylation sites.…”

“…However, studies performed on the gastric pathogen Helicobacter pylori confirm that isogenic variants that express Lewis x and Lewis y sequences on their lipopolysaccharides induce tolerance in the human gastric mucosa (71)(72)(73). This deviation of the human immune response is associated with specific shifts in cytokine expression by naïve dendritic cells (DCs) and T cell polarization from a Th1 toward a Th2 response (70). These effects are dependent on signaling mediated by the interaction of these lipopolysaccharides with DC-SIGN (71,74).…”

Lectinomics I. Relevance of exogenous plant lectins in biomedical diagnostics

“…The order of the affinity purification affects the composition of enriched sample and combinations include (but are not limited) for example Con A and phytohaemagglutinin E column, Con A and pea lectin (PSA) or WGA columns (Yoshida et al 1995(Yoshida et al , 1997Sumi et al 1999). Recently, this experimental approach was further expanded to identify sialylated glycoprotein markers in serum of pancreatic cancer patients (Zhao et al 2006).…”

“…Gel columns containing lectins Con A, PHA-E, PHA-L, PSA and WGA were used to investigate γ-glutamyltransferase (GGT) in human renal cell carcinoma (RCC) (Yoshida et al 1995), to detect altered Asn-linked saccharide chain structure of prostatic acid phosphatase (Yoshida et al 1997) and prostate-specific antigen . Alterations of Asn-linked saccharides on N-acetyl-β-Dhexosaminidase during renal oncogenesis were studied using serial lectin affinity chromatography with Con Aand WGA ).…”

Lectin-binding sites in epithelial cells of the mouse prostate gland

“…Lectins bind not only to sugar chains, but also to proteins, lipids, and other small molecules and support various physiological processes in a variety of cell types. Thus, they have been widely used for detecting sugar chains in histochemical studies and for characterizing prostatic cells in several species, including humans [9][10][11][12] , rats [13][14][15][16] , mice 5,17) , goats 7,8) , rhesus monkeys 18) , and water buffalos 19) .…”