Serial semithin sections in immunohistochemistry : Techniques and applications.

Grube, D.; Kusumoto, Yoshisuke

doi:10.1679/aohc.49.391

Cited by 66 publications

(40 citation statements)

References 35 publications

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“…B. A. S., ZeissKontron, Germany; see GRUBE and KUSUMOTO, 1986;EHRHART et al, 1988). According to the findings so far obtained in G and A cells, a relatively high proportion of these cells exhibits this reciprocal relation between CgA-and peptide-immunoreactivities.…”

Section: Interrelations Of Cga With Peptide Hormonesmentioning

confidence: 88%

“…We used a limited number of polyclonal antisera against bovine and rat Cg (source: D. Aunis, Strasbourg, France; H. Winkler, Innsbruck, Austria) that have been characterized immunologically and immunohistochemically (EHRHART et al, 1986(EHRHART et al, , 1988HAGN et al, 1986;FISCHER-COLBRIE and SCHOBER, 1987;YOSHIE et al, 1987;BARGSTEN et al, 1988). The tissues under study and reference organs were prepared by identical, established methods (GRUBE and KUSUMOTO, 1986). Throughout all studies, the peroxidase anti-peroxidase (PAP) technique (STERNBERGER, 1986) was applied according to generally standardized protocol (GRUBE and KUSUMOTO, 1986).…”

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

“…The tissues under study and reference organs were prepared by identical, established methods (GRUBE and KUSUMOTO, 1986). Throughout all studies, the peroxidase anti-peroxidase (PAP) technique (STERNBERGER, 1986) was applied according to generally standardized protocol (GRUBE and KUSUMOTO, 1986). Our investigations were done on serial semithin (0.5um) sections, alternatively immunostained for Cg and amines or peptides.…”

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

“…Thus, endocrine cells immunoreactive for Cg could be clearly identified. Moreover, immunoreactivities in semithin sections can be quantified or compared, with immunoreactive cells serving for reference (GRUBE and KUSUMOTO, 1986;EHRHART et al, 1988).…”

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

See 3 more Smart Citations

Chromogranins in mammalian GEP endocrine cells: Their distribution and interrelations with co-stored amines and peptides.

Grube

Bargsten

Cetin

et al. 1989

Archives of Histology and Cytology

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Section: Interrelations Of Cga With Peptide Hormonesmentioning

confidence: 88%

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

Section: Concerning the Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Chromogranins in mammalian GEP endocrine cells: Their distribution and interrelations with co-stored amines and peptides.

Grube

Bargsten

Cetin

et al. 1989

Archives of Histology and Cytology

Self Cite

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“…Serial sections of 0.5 μm thickness were cut from the tissue blocks with an ultramicrotome and mounted on microscope glass slides. After the removal of the resin by sodium methoxide (Grube and Kusumoto, 1986), semithin sections were treated with a 0.05% citraconic anhydride solution (Immunosaver; Nissin EM Co., Ltd., Tokyo, Japan) for 30 min at 60°C as an antigen retrieval procedure (Namimatsu et al, 2005), and then incubated with 2% normal donkey serum (30 min, 20°C) for blocking. After these pretreatments, tissue sections were incubated with a mixture of primary antibodies of different species (rabbit-, mouse-, sheep-/goat-origin) for 16 h at 20°C.…”

Section: Immunofluorescence Microscopymentioning

confidence: 99%

Sustained treatment with a GnRH agonist (leuprorelin) affects the ultrastructural characteristics of membranous organelles in male rat pituitary gonadotropes

Bochimoto

Koga

Sakai

et al. 2013

Archives of Histology and Cytology

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Summary. Gonadotropin-releasing hormone (GnRH) agonists exert acutely stimulatory action on gonadotropes, but thereafter suppress paradoxically gonadotropin synthesis and release by receptor desensitization. To examine whether GnRH signaling affects the morphological characteristics in membranous organelles related to the synthesis of gonadotropin, we have analyzed the ultrastructural changes in the ER and Golgi apparatus of male rat pituitary gonadotropes during sustained treatment with a GnRH agonist, leuprorelin. In pituitary gonadotropes at 1 day after the onset of treatment, clusters of the tubuloreticular ER appeared, and the globular Golgi apparatus was transiently disassembled into isolated small-sized stacks. However, 1 week after the onset of the treatment, the tubuloreticular ER was seemingly converted to rough ER with regularly stacked sheets and the scattered Golgi stacks converged to form globular structures. In the following chronic phase of the treatment, the ER cisterns remained flattened and the trans-Golgi compartment appeared to be collapsed. Sustained treatment with leuprorelin could also restore the enlarged Golgi apparatus and expand the cisterns of the rough ER; a feature that was seen in hypertrophic gonadotropes of castrated rats. These findings indicated that the ultrastructure of the membranous organelles changed because of the chronic suppressive effects of leuprorelin on gonadotropes both in the physiological and stimulated states. The acute and chronic ultrastructural changes in the ER and Golgi apparatus during sustained leuprorelin treatment also suggests that GnRH signaling cross-talks with the regulation of the morphological characteristics in membranous organelles related to gonadotropin synthesis.

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Fine structure and immunocytochemistry of cells within the endocrine pancreas of larval and adult sea lampreys, Petromyzon marinus L.

Elliott

Youson

1988

Am. J. Anat.

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Immunocytochemistry with protein A-gold and routine electron microscopy were used to identify cell types within the endocrine pancreas of larvae, juvenile adults, and upstream-migrant adults of the sea lamprey, Petromyzon marinus. The larval pancreatic islets are composed only of insulin-immunoreactive B-cells, which are uniform in their fine structure. The cranial and caudal pancreatic tissue in both adult periods contains three cell types: B-cells, somatostatin-immunoreactive D-cells, and a third cell type of unknown content. No glucagon-immunoreactive cells are present in lampreys, but B- and D-cells exist in equal numbers in the pancreatic tissue of adults. The B-cells of adults have a fine structure similar to those in larvae. D-cells have secretory granules that are distinctly different from those both in B-cells and in the third cell type. Although B- and D-cells in lamprey pancreatic tissues have a basic morphological similarity to these cells in other vertebrates, their granules are generally of smaller dimensions. The inclusion of granules within large pleomorphic bodies in many D-cells indicates that granule turnover is common. Immunocytochemistry will be a useful tool for showing the relationship between the cells in the degenerating bile ducts and those of the developing adult pancreas.

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Serial semithin sections in immunohistochemistry : Techniques and applications.

Cited by 66 publications

References 35 publications

Chromogranins in mammalian GEP endocrine cells: Their distribution and interrelations with co-stored amines and peptides.

Chromogranins in mammalian GEP endocrine cells: Their distribution and interrelations with co-stored amines and peptides.

Sustained treatment with a GnRH agonist (leuprorelin) affects the ultrastructural characteristics of membranous organelles in male rat pituitary gonadotropes

Fine structure and immunocytochemistry of cells within the endocrine pancreas of larval and adult sea lampreys, Petromyzon marinus L.

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