Serial Transmission in Suckling Mice of a Virus from Cattle with Bovine Epizootic Fever

Sasaki, Norimasa; Kodama, Kazuo; Iwamoto, Ichizo; Izumida, Akihiro; Matsubara, Toshiyuki

doi:10.1111/j.1348-0421.1968.tb00391.x

Cited by 12 publications

(16 citation statements)

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“…The virus of bovine ephemeral fever has been successfully propagated in cattle [6,9], suckling mice [2,5,6,[11][12][13]16], suckling hamsters [5,6], and suckling rats [5,6]. In the present study, additional data on the susceptibility of animals to the virus were obtained.…”

Section: Discussionsupporting

confidence: 60%

“…Significant progress, however, was made recently when their causative agents were propagated in small laboratory animals. Van der Westhuizen [16] in South Africa, Doherty et al [2] and Spradbrow and Francis [13] in Australia and Sasaki et al [11] in Japan recovered viruses in suckling mice. Inaba and his associates [5,6] 413 in Japan recovered a virus in suckling hamsters, and could further passage the virus in suckling mice and rats and in cultures of BHK21-WI2 cells of baby hamster kidney origin.…”

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confidence: 99%

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Behavior of Bovine Ephemeral Fever Virus in Laboratory Animals and Cell Cultures

Matumoto

Inaba

Tanaka

et al. 1970

Japanese Journal of Microbiology

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A Japanese strain (Yamaguchi) of bovine ephemeral fever virus, propagated in BHK21-WI2 cells of hamster kidney origin, was used. Adult rats inoculated intracardially or intracerebrally remained healthy, but produced neutralizing antibody, suggesting actual viral growth in the animals. However, no clinical and serological evidence for infection with the virus was obtained in adult sheep, goats, swine and rabbits inoculated intravenously.One-day-old guinea pigs inoculated intracerebrally and adult guinea pigs inoculated intracardially remained healthy and produced no neutralizing antibody. The virus multiplied not only in BHK21-WI2 cell culture but also in cultures of stable line hamster cells (HmLu-1 and HmT), primary hamster kidney and embryo cells, long-term cultured bovine embryo cells (BEK1), primary calf kidney and embryo cells, and primary suckling rat kidney and embryo cells. However, no evidence for cytopathogenesis and viral growth was obtained in primary cultures of bovine leukocytes, bovine bone marrow, thymus, thyroid and adrenal cells, guinea pig, mouse and chick kidney cells, and mouse and chick embryo cells, and in culture of African green monkey kidney cells (Vero). The virus multiplied best and induced marked cytopathogenesis in BHK21-WI2 cells; lower temperatures such as 34 C or 30 C appeared preferable to obtain constantly better viral yields, although the virus grew more slowly. Similar effects of the incubation temperatures were also obvious in the other susceptible cell cultures, in which the cytopathogenesis was less marked than in BHK21-WI2 cells or absent, roughly coinciding with decreasing grades of viral growth.

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Section: Discussionsupporting

confidence: 60%

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confidence: 99%

Behavior of Bovine Ephemeral Fever Virus in Laboratory Animals and Cell Cultures

Matumoto

Inaba

Tanaka

et al. 1970

Japanese Journal of Microbiology

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“…The causative agent of bovine epizootic fever has been identified and is distinct from Ibaraki virus [12,13,19].…”

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Ibaraki Virus, an Agent of Epizootic Disease of Cattle Resembling Bluetongue

Omori

Inaba

Morimoto

et al. 1969

Japanese Journal of Microbiology

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Outbreaks of an acute febrile disease of cattle occurred in Japan in 1959 and 1960. Its occurrence was limited in late summer and autumn, and in Kyushu, Shikoku and Honshu roughly south of 37 degrees north latitude, suggesting a close correlation of the incidence with the climatic conditions, hence a possibility of the presence of arthropod vector. The disease was characterized by fever and lesions affecting the mucous membrane and skin, musculature and vascular system. Degeneration of striated muscles was observed in the esophagus, larynx, pharynx, tongue and skeletal muscular system. Edema and hemorrhage were marked in the mouth, lips, abomasum, coronets etc., occasionally followed by degeneration of the epithelium leaving erosions or ulcerations. Severe lesions affecting the esophageal and laryngopharyngeal musculature caused deglutitive difficulty which in turn resulted in dehydration and emaciation, and occasionally in aspiration pneumonia, constituting the major causes of death of the affected animals. These findings indicate that the disease resembles bluetongue in sheep and cattle. The clinical materials obtained from natural cases induced a clinical illness when inoculated into calves, and the disease was transmitted serially in calves by intravenous inoculation of the blood obtained at the height of febrile reaction. The experimentally produced disease was clinically and pathologically indistinguishable from the natural disease.

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“…Van der Westhuizen [4] in South Africa recently reported the isolation of a virus in suckling mice from natural and experimentally produced cases of ephemeral fever, and gave evidence for the virus to be the cause of the disease. Sasaki et al [3] and Inaba et al [1,2] in Japan also succeeded in isolation of a virus in suckling mice and hamsters from an experimentally produced case of bovine epizootic fever. The South African and Japanese viruses resemble each other in their pathogenicity for suckling mice, rapid loss of pathogenicity for cattle upon serial passage in suckling mice, and sensitivity to ether and deoxycholate.…”

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confidence: 99%