Serial transplantation of a human T-cell acute lymphoblastic leukaemia line into nude mice

Miyoshi, Isao; Ota, Takuji; Hiraki, Shunkichi; Sumida, Mitsuo; Tanaka, Toshio; Kimura, Ikuo

doi:10.1038/bjc.1981.157

Cited by 5 publications

(2 citation statements)

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“…It has been difficult to obtain tumors in nude mice by inoculation of malignant human hemopoietic cells (39,40). Our cell line did indeed produce tumors in unirradiated nude mice without coinjection of other malignant cells (40).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Helgestad¹,

Pettersen²,

Storm‐Mathisen³

et al. 1990

European J of Haematology

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A new malignant human T‐cell line‐labelled PFI‐285‐has been isolated from a boy with malignant lymphoma. Morphologically, the cells had characteristics of malignant lymphoid cells. The cells presented surface antigens as early cortical lymphocytes and proliferated non‐adherently as single cells, independent of T‐cell growth factor (IL‐2), in liquid culture. The cells had undetectable levels of receptors for IL‐2, were not clonogenic in soft agar, but did form tumors in nude mice. Their establishment and continuous growth in vitro was dependent on the number of cells inoculated and on the growth medium used. Cytogenetic alteration, HTLV‐1 or reverse transcriptase activity were not detected. The production of known T‐cell derived lymphokines such as IL‐2, B‐cell growth factor(s), α‐interferon or granulocyte/macrophage colony stimulating or inhibiting factor(s) was not detected. The cells had 5–8% natural killer (NK)‐cell activity against NK‐cell sensitive target cells (K562) and were not sensitive for NK cells. A most unusual characteristic was the pronounced sensitivity of the cells to ascorbic acid. Concentrations down to 50 μmol/l killed the cells within hours.

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Section: Discussionmentioning

confidence: 99%

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Helgestad¹,

Pettersen²,

Storm‐Mathisen³

et al. 1990

European J of Haematology

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“…Leukemia and lymphoma cells usually grow in nude mice as localized tumors (Povlsen et al, 1973;Machado et a/. , 1977;Ueyama et al, 1977) and rarely show disseminated growth (Miyoshi et a / . , 1981).…”

Section: Discussionmentioning

confidence: 99%

Direct and serial transplantation of Ph¹‐positive acute lymphoblastic leukemia into nude mice

Miyagi

Kubonishi

Ohtsuki

et al. 1989

Intl Journal of Cancer

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A new Philadelphia chromosome (Ph1-positive) acute lymphoblastic leukemia (ALL) cell line was established in nude mice by direct and serial transplantation of peripheral blood leukemia cells from an adult patient. Although the patient's leukemia cells did not grow in vitro, they were successfully transplanted for 8 serial passages, giving rise to progressive growth of tumors with frequent involvement of lymph nodes, liver, spleen, bone marrow, and meninges. The tumor cells could also be passaged in an ascites form. This in vivo cell line, designated PALL-I, retained the Ph1 chromosome, t(9;22) (q34;q11), and pre-B-cell phenotype (SmIg-, CpIg-, CD10+, CD19+, OKIaI+, and CD38+), like the original leukemia cells. Molecular genetic analysis of PALL-I cells revealed neither bcr rearrangement nor 8.5-kb abI-related mRNA that is characteristically seen in Ph1-positive chronic myelogenous leukemia (CML). Thus, the PALL-I cell line is genetically distinct from CML. It may provide a useful model for an understanding of the cellular and molecular biology of Ph1-positive ALL without classical bcr rearrangement.

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Tall-1

Drexler¹

2001

The Leukemia-Lymphoma Cell Line FactsBook

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Serial transplantation of a human T-cell acute lymphoblastic leukaemia line into nude mice

Abstract: Images Fig. 1 Fig. 2 Fig. 3

Cited by 5 publications

References 5 publications

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Direct and serial transplantation of Ph¹‐positive acute lymphoblastic leukemia into nude mice

Tall-1

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Serial transplantation of a human T-cell acute lymphoblastic leukaemia line into nude mice

Abstract: Images Fig. 1 Fig. 2 Fig. 3

Cited by 5 publications

References 5 publications

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Direct and serial transplantation of Ph1‐positive acute lymphoblastic leukemia into nude mice

Tall-1

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Direct and serial transplantation of Ph¹‐positive acute lymphoblastic leukemia into nude mice