Sericin Enhances Attachment of Cultured Human Skin Fibroblasts

Tsubouchi, Kozo; Igarashi, Yumiko; Takasu, Yoko; Yamada, Hiromi

doi:10.1271/bbb.69.403

Cited by 208 publications

(140 citation statements)

References 6 publications

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“…These findings also differ from Tsubouchi et al (2005) where the day3 count for silk-sericin-treated fibroblasts was significantly enhanced, compared with the control. They attributed this to the acceleration of initial cell attachment.…”

Section: Fibroblast Growthcontrasting

confidence: 82%

In-vitro Characterization of Silk Sericin as an Anti-aging Agent

Kitisin¹,

Maneekan²,

Luplertlop³

2013

JAS

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Silk sericin is a natural macromolecular protein derived from the silkworm, Bombyx mori. It is also a by-product of silk-making. From previous reports, many cosmeceuticals and other cosmetic products have been developed with silk sericin. This study aimed to investigate an anti-aging property of silk sericin by in-vitro characterization using fibroblast cell culture model. The results showed that silk sericin can stimulate collagen type I synthesis, suppress the regulation of nitrite, which nitrite may induces oxidative stress, and up-regulate the expression of b-cell lymphoma 2 (bcl-2) to inhibit cell apoptosis, without altering fibroblast growth kinetics or cellular ultrastructure. Sericin anti-aging properties were comparable to vitamin C, except for oxidative stress, where silk sericin was superior. The results suggested that silk sericin possesses anti-aging properties that could be usefully incorporated into high-quality cosmetics, cosmeceuticals, and food supplements.

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Section: Fibroblast Growthcontrasting

confidence: 82%

In-vitro Characterization of Silk Sericin as an Anti-aging Agent

Kitisin¹,

Maneekan²,

Luplertlop³

2013

JAS

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“…Specifically, sericin is highly resistant to oxidation (Suzuki et al, 2004), is UV resistant (Sarovart et al, 2003), and has a moisturizing effect on skin (Padamwar et al, 2005). In addition, Tsubouchi et al (2005) reported that sericin improves the attachment of cultured human skin fibroblasts. Nagai et al (2009) revealed that sericin can promote wound healing.…”

Section: Introductionmentioning

confidence: 99%

Effect of centrifugation on the structure and properties of silk sericin

Park¹,

Um²

2016

International Journal of Industrial Entomology

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Recently, silk sericin has been studied extensively for biomedical and cosmetic applications because of its unique properties, including UV resistance and wound healing ability. For use in applications, sericin is fabricated in various forms including films and gels. However, the mechanical properties of sericin are too weak. In this basic study on improving the mechanical properties of sericin, a silk sericin aqueous solution was separated into two layers by centrifugation. The solution viscosity, molecular conformation, and mechanical properties of each separation layer of the sericin were examined. Sericin from the lower layer had a higher solution viscosity and film mechanical properties (strength and strain) than that from the upper layer, implying that sericin from the lower layer had a higher molecular weight than that from the upper layer. The molecular conformation of the sericin films varied depending on the casting solvent. In aqueous solution, the sericin film from the lower layer showed a b-sheet conformation, whereas that from the upper layer displayed a random coil conformation. All the sericin films showed a highly b-sheet-crystallized state when cast in formic acid, regardless of the separation layer.

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“…However, silk sericin was recently reported to exhibit inhibition of ultraviolet B-induced apoptosis in human skin keratinocytes (Dash et al, 2008), high water retention, wound-healing properties (Nagai et al, 2009), cyto-compatibility (Tsubouchi et al, 2005), and blood-glucose-and cholesterol-lowering effects (Limpeanchob et al, 2010;Seo et al, 2011). Therefore, researchers are studying sericin for various biotechnological applications, including cosmetics and wound dressing.…”

Section: Introductionmentioning

confidence: 99%

Structure and properties of silk sericin obtained from different silkworm varieties

Chung¹,

Lee²,

Kweon³

et al. 2015

International Journal of Industrial Entomology

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Recently, many researchers have studied silk sericin because of its high water retention, good wound healing, good cyto-compatibility, and blood-glucose-and cholesterol-lowering effects. Although sericin film can be used in wound dressing and cosmetic packs, its poor mechanical properties have prevented its use in industrial fields. In the present study, sericin was obtained from different silkworm varieties, and the effect of silkworm variety on the structure and properties of sericin was examined. Except for a small difference in serine content, no significant difference in sericin was noted among the silkworm varieties. In addition, silkworm variety almost had no effect on solution viscosity, implying that it does not influence the molecular weight of sericin. Mechanical properties of sericin film were strongly affected by silkworm variety. Wonwon 126 showed the best mechanical properties, while N74 and Geumgwangju displayed the worst properties. IntroductionRemoval of sericin from silk fibers improves the luster and feel of silk textiles, and so, sericin was considered a useless protein.However, silk sericin was recently reported to exhibit inhibition of ultraviolet B-induced apoptosis in human skin keratinocytes (Dash et al., 2008), high water retention, wound-healing properties (Nagai et al., 2009), cyto-compatibility (Tsubouchi et al., 2005, and blood-glucose-and cholesterol-lowering effects (Limpeanchob et al., 2010;Seo et al., 2011). Therefore, researchers are studying sericin for various biotechnological applications, including cosmetics and wound dressing.Sericin film can be used in various applications. However, sericin film is too brittle, showing poor mechanical properties that restrict its application in various biotechnological fields.Recently, Jo and Um (2015) reported that formic acid casting can induce a two-fold increase in the mechanical properties of sericin film. However, the mechanical properties of sericin film need to be enhanced to a greater extent for biomedical and cosmetic applications.Silk is produced by the silkworm (Bombyx mori), and many varieties of B. mori exist. Recently, Chung et al. (2015) reported that molecular weight (MW), solution viscosity, and film mechanical properties of regenerated silk fibroin (SF) were significantly different depending on the silkworm variety. They suggested the possibility of improving the mechanical properties of sericin film.In the present study, nine different B. mori varieties were used

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Sericin Enhances Attachment of Cultured Human Skin Fibroblasts

Cited by 208 publications

References 6 publications

In-vitro Characterization of Silk Sericin as an Anti-aging Agent

In-vitro Characterization of Silk Sericin as an Anti-aging Agent

Effect of centrifugation on the structure and properties of silk sericin

Structure and properties of silk sericin obtained from different silkworm varieties

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