Serine/Threonine Protein Phosphatases type 2A and their roles in stress signaling

País, Silvia Marina; Téllez-Iñón, Marı́a T.; Capiati, Daniela Andrea

doi:10.4161/psb.4.11.9783

Cited by 96 publications

(58 citation statements)

References 37 publications

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“…3). It is remarkable to note that the H þ -ATPase is dephosphorylated by a protein phosphatase 2A (PP2A) (Camoni et al, 2000), which has been reported to be involved in cold stress response (Pais et al, 2009;Zhao, 2015).…”

Section: Discussionmentioning

confidence: 99%

Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

Muzi

Camoni

Visconti

et al. 2016

Plant Physiology and Biochemistry

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a b s t r a c tLow temperature is an environmental stress that greatly influences plant performance and distribution. Plants exposed to cold stress exhibit modifications of plasma membrane physical properties that can affect their functionality. Here it is reported the effect of low temperature exposure of Arabidopsis plants on the activity of phospholipase D and H þ -ATPase, the master enzyme located at the plasma membrane.The H þ -ATPase activity was differently affected, depending on the length of cold stress imposed. In particular, an exposure to 4 C for 6 h determined the strong inhibition of the H þ -ATPase activity, that correlates with a reduced association with the regulatory 14-3-3 proteins. A longer exposure first caused the full recovery of the enzymatic activity followed by a significant activation, in accordance with both the increased association with 14-3-3 proteins and induction of H þ -ATPase gene transcription. Different time lengths of cold stress treatment were also shown to strongly stimulate the phospholipase D activity and affect the phosphatidic acid levels of the plasma membranes. Our results suggest a functional correlation between the activity of phospholipase D and H þ -ATPase mediated by phosphatidic acid release during the cold stress response.

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Section: Discussionmentioning

confidence: 99%

Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

Muzi

Camoni

Visconti

et al. 2016

Plant Physiology and Biochemistry

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“…PP2A belongs to a major Ser/Thr phosphatase family in eukaryotic cells, and it plays crucial roles in numerous signaling pathways in animals and plants by regulating the dephosphorylation to affect the substrate conformation, cellular localization, activity, or stability (Janssens and Goris, 2001;País et al, 2009). The PP2A holoenzyme is composed of three subunits: the scaffolding subunit A that brings together the regulatory subunit B and the catalytic subunit C. There are three A genes, 17 B genes, and five C genes for PP2A subunits in Arabidopsis (Arabidopsis thaliana; Farkas et al, 2007).…”

mentioning

confidence: 99%

“…The PP2A holoenzyme is composed of three subunits: the scaffolding subunit A that brings together the regulatory subunit B and the catalytic subunit C. There are three A genes, 17 B genes, and five C genes for PP2A subunits in Arabidopsis (Arabidopsis thaliana; Farkas et al, 2007). The five PP2A catalytic subunit genes are classified into two subfamily groups (Farkas et al, 2007;País et al, 2009): subfamily I, including PP2A-C1, PP2A-C2, and PP2A-C5, and subfamily II, including PP2A-C3 and PP2A-C4. Overexpression of PP2A-C2 decreases ABA sensitivity and alters plant response to drought, high salt, and sugar stresses, indicating that this subunit is negatively involved in ABA signaling (Pernas et al, 2007).…”

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confidence: 99%

TAP46 Plays a Positive Role in the ABSCISIC ACID INSENSITIVE5-Regulated Gene Expression in Arabidopsis

Zhu

Shen

et al. 2013

Plant Physiology

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TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in ABA-regulated seed maturation and seedling development. Expression of TAP46 coincides with the action of ABA in developing seeds and during seed germination, and the TAP46 transcript reaches to the highest level in mature seeds. Real-time polymerase chain reaction analysis indicates that external ABA can increase TAP46 transcript level transiently during seed germination. Overexpression of TAP46 increases plant sensitivity to ABA, while tap46 knockdown mutants are less sensitive to ABA during seed germination, suggesting that TAP46 functions positively in ABA signaling. Overexpression of TAP46 also leads to lower PP2A activity, while tap46-1 knockdown mutant displays higher PP2A activity, suggesting that TAP46 negatively regulates PP2A activity in Arabidopsis. Both TAP46 and PP2A interact with the ABA-regulated transcription factor ABA INSENSITIVE5 (ABI5) in vivo, and TAP46's binding to ABI5 can stabilize ABI5. Furthermore, TAP46's binding to the phosphorylated ABI5 may prevent PP2A or PP2A-like protein phosphatases from removing the phosphate from ABI5, thereby maintaining ABI5 in its active form. Overexpression of TAP46 and inhibition of activities of PP2A or PP2A-like protein phosphatases can increase transcript levels of several ABI5-regulated genes, suggesting that TAP46 is a positive factor in the ABA-regulated gene expression in Arabidopsis.

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“…In Arabidopsis thaliana, PP2A has been implicated in auxin transport (Ballesteros et al 2013), abscisic acid and ethylene signaling (Kwak et al 2002;Pernas et al 2007), growth and development (Zhou et al 2004;Blakeslee et al 2008) and control of the cytoskeletal structure (Camilleri et al 2002). Recently, PP2A transcription showed to be upregulated by fungal elicitors in potato and tomato (País et al 2009) as well as in sunflower after inoculation with a necrotrophic fungus (Alignan et al 2006). Activation of these protein phosphatases can produce the phosphorylation cascade which leads to the oxidative burst and localized cell death (as evident also in the typical Rvi1-associated necrosis).…”

Section: Discussionmentioning

confidence: 97%

High-resolution genetic and physical map of the Rvi1 (Vg) apple scab resistance locus

et al. 2015

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Apple scab, caused by the fungal pathogen V. inaequalis, is one of the most prejudicial diseases in apple (Malus x domestica Borkh.). At least 17 monogenic resistances against this pathogen have been identified in the Malus genus, but only two, Rvi6 (Vf) and Rvi15 (Vr2), have been cloned and functionally characterized so far. Here, we describe the first steps of the cloning of a new scab resistance gene, Rvi1 (Vg). This gene is carried by the well-known cultivar 'Golden Delicious' and confers specific resistance against V. inaequalis strains carrying the AvrRvi1 avirulence factor. With a fine mapping approach on a population of 1,983 individuals, we developed SSR markers tightly linked to the Rvi1 gene. Sequencing of two bacterial artificial chromosome (BAC) clones covering, respectively, the Rvi1 apple scab resistant and susceptible allele, allowed the identification of a 110-kilo base pairs (kbps) sequence flanked by two linked markers (Vg12- and Vg15-SSR). Open reading frames were predicted on these BACs: four TIR-NBS-LRR (TNL) putative genes, a putative TNL pseudogene and one serine/threonine protein phosphatase 2A were identified

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Serine/Threonine Protein Phosphatases type 2A and their roles in stress signaling

Cited by 96 publications

References 37 publications

Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

TAP46 Plays a Positive Role in the ABSCISIC ACID INSENSITIVE5-Regulated Gene Expression in Arabidopsis

High-resolution genetic and physical map of the Rvi1 (Vg) apple scab resistance locus

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