Serious Infections With Edwardsiella tarda

Wilson, Jo P.; Waterer, Rebecca R.; Wofford, John D.; Chapman, Stanley W.

doi:10.1001/archinte.1989.00390010170025

Cited by 70 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Edwardsiella tarda causes a septicaemia infection (edwardsiellosis) in a variety of cultured freshwater and marine fish species worldwide (Plumb, 1999;Muroga, 2001), and the bacterium is also known as a cause of gastro-and extraintestinal infections in humans (Jordan and Hadley, 1969;Wilson et al, 1989). In Japan, fish edwardsiellosis was first reported as the disease in cultured Japanese eel Anguilla japonica (Wakabayashi and Egusa, 1973).…”

mentioning

confidence: 99%

Induction of Hemagglutinating Activity of Edwardsiella tarda by Sodium Chloride

Yasunobu¹,

Arikawa²,

Furutsuka³

et al. 2006

Fish Pathol.

View full text Add to dashboard Cite

ABSTRACT-The hemagglutinating activity (HA) of Edwardsiella tarda, which had been isolated from cultured fish and culture environments, was investigated in relation to NaCl concentration of the growth medium. E. tarda cells were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) and without NaCl (0%-NaCl culture). Hemagglutination assays with guinea pig erythrocytes classified the strains into three HA patterns. Seventeen strains exhibited HA only with the 3%-NaCl culture (type A). A more frequent type (35 strains) displayed HA in both 0%-and 3%-NaCl cultures but the 3%-NaCl culture showed higher HA activity than the 0%-NaCl culture (type B). No HA was detected in both cultures of the other three strains (type C). The NaCl-induced HA was well correlated with the expression of a 19.3 kDa protein, a fimbrial major subunit (FimA). Infection experiments with a selected strain (type A) of E. tarda revealed that the 3%-NaCl culture was more virulent to Japanese flounder Paralichthys olivaceus than the 0%-NaCl culture, when fish were challenged by an oral route. This induction of the fimbrial protein under higher NaCl concentration may play an important role in the virulence of E. tarda in marine environments.

show abstract

mentioning

confidence: 99%

Induction of Hemagglutinating Activity of Edwardsiella tarda by Sodium Chloride

Yasunobu¹,

Arikawa²,

Furutsuka³

et al. 2006

Fish Pathol.

View full text Add to dashboard Cite

show abstract

“…Since then, the organism has also been isolated from environmental water (17,31) and a variety of animals: fish (1,15,19,29), reptiles, birds and mammals (28) including humans (5,30). E. tarda is the etiological agent of edwardsiellosis, a serious infectious systemic disease in freshwater and marine fish species, including many commercially important fish such as chinook salmon (1), flounder, plaice (15), carp (19) and eel (29).…”

mentioning

confidence: 99%

Vaccine Efficacy of Recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis

Liu

Oshima

Kurohara

et al. 2005

Microbiology and Immunology

View full text Add to dashboard Cite

Thirty-seven kilodalton glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Edwardsiella tarda was suggested to be an effective vaccine candidate against E. tarda infection in previous research. For developing a vaccine, obtaining GAPDH in large quantities is necessary. In this study, we determined the complete nucleotide sequence of the gene that encodes GAPDH of E. tarda, and overexpressed the GAPDH of E. tarda by using the Escherichia coli expression system. We immunized Japanese flounder with recombinant GAPDH (rGAPDH) and evaluated its vaccine efficacy. Our results showed that rGAPDH effectively protected Japanese flounder from experimental E. tarda infection, and will contribute to the development of a vaccine against E. tarda.

show abstract

“…Infection by E. tarda in epidemic proportions has been reported to have a disastrous effect on fish culture. E. tarda has also been recognized as a serious human pathogen causing a variety of clinical syndromes (13). Among several virulence factors studied with E. tarda (5,12), hemolysis by hemolysins is considered to be a major cause of death in the infected fish.…”

mentioning

confidence: 99%

Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

Savan

Igarashi

Matsuoka

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65°C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.

show abstract

Serious Infections With Edwardsiella tarda

Cited by 70 publications

References 22 publications

Induction of Hemagglutinating Activity of Edwardsiella tarda by Sodium Chloride

Induction of Hemagglutinating Activity of Edwardsiella tarda by Sodium Chloride

Vaccine Efficacy of Recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis

Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

Contact Info

Product

Resources

About