Sero-epidemiological value of some hydatid cyst antigen in diagnosis of human cystic echinococcosis

Abstract

Cystic echinococcosis (CE) is a severe zoonotic disease which affects both human and animals. The disease has a considerable economic and social impact, because it has numerous complications leading to important disabilities and even death. CE is a widespread chronic endemic helminthic disease caused by infection with metacestodes of tapeworm Echinococcus granulosus. This study was conducted to diagnosis human CE by hydatid cyst antigens from camels and sheep. Hydatid fluid and protoscoleces crude antigens corresponding to camel and sheep were resolute by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and the protein bands of different antigens were exposed to infected patients serum CE through western blot (WB) assay. The camel hydatid fluid antigen revealed five polypeptide bands of 18-98.8 kDa by SDS-PAGE while sheep hydatid fluid antigen revealed four polypeptide bands of 20-100 kDa. Immune reactive bands were obtained through WB ranged from 25 to 125 kDa. The study showed prominent immune reactive bands of 92, 52.2 and 35.7 kDa which may helpful in diagnosis of human CE.

Immunological detection of human and camel cystic echinococcosis using different antigens of hydatid cyst fluid, protoscoleces, and germinal layers

“…However, CE is still a common health problem in developing countries, particularly in rural areas [28]. Community-based studies have demonstrated that the prevalence of human CE is between 1% and 10% in endemic regions [14]. Notably, 22.6% of slaughtered camels came from the Northern and Southern Egyptian slaughterhouses [29].…”

“…The use of crude hydatid cyst antigens in serological diagnostic tests is inadequate. Thus, the purification of hydatid cyst antigens excludes other cross-reactive proteins [14]. Many types of electrophoretic analysis have been used to purify hydatid antigens.…”

“…Furthermore, indirect ELISA is beneficial in primarily diagnosing CE and in post-treatment follow-up by detecting anti-E. granulosus IgG [13]. Camel hydatid fluid, protoscoleces-crude antigens, and sheep hydatid cysts may be helpful for efficient hydatidosis diagnoses in humans [14].…”

Opportunistic Parasitic Pulmonary Infections in Human Immunodeficiency Virus (Hiv) Infected Patients: With References to Egyptian Parasites

“…In Egypt, many authors dealt with the definitive host, mainly dogs, cattle, slaughtered an-imals and human infection as well as the medical and surgical treatment (Handousa, 1951;Haridy et al, 1998;2000, 2008El Shazly et al, 2007a;Aaty et al, 2012;Hassanain et al, 2016;Aldahmashi et al, 2016; and others) Conclusion It is important to consider parasitic infections in the differential diagnosis of such lung diseases. If identified early, most parasitic diseases that affect the lung are curable with medical or surgical treatments.…”

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

“…The cyst fluid (CF) and protoscolex proteins of Eg had been evaluated and proved to be valuable antigens for the CE serological diagnosis [16,22,24]. However, sensitivities and specificities of these antigen proteins differ greatly, and some of the cyst fluid antigen components such as metabolites and host tissue components can cross-react with nonspecific antibodies that target other worms, resulting in poor specificity [15,22]. Golassa et al [17] evaluated the diagnostic value of crude cyst fluid in bovine CE via indirect hemagglutination test (IHA) and found that the false positive and false negative rates were high, and the accuracy is relatively low.…”