Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

Abstract

Ornithobacterium rhinotracheale is a gram negative bacterial pathogen causing respiratory tract infections in poultry. Tracheal, lung and serum samples were obtained from 21 broiler flocks of 8 farms from a slaughterhouse located in south-eastern of Iran. Among 630 tracheal and lung samples from samples resulting from 315 chickens, 11 (3.5%) ORT isolates were identified using biochemical tests. The isolates originated from 9 (42.9%) flocks out of 4 farms. All of the isolates were recovered from tracheal swabs and showed an API 20NE identification biocode 0-2-2-0-0-0-4. Of the 420 serum samples examined by ELISA, 134 (31.9%) sera from 17 (81.0%) flocks were positive for ORT antibodies. These results indicate that ORT is present in most broiler flocks with respiratory disorders in southeast Iran.

Molecular identification of Ornithobacterium rhinotracheale in turkeys in Isfahan province of Iran

“…All ORT isolates that were positive by the culture were also detected to be positive by the PCR (Ozbey et al, 2004). Koga et al, (2005) (Ghanbarpour and Salehi, 2009). Since this study was carried out throughout the fall in 2010 (Isfahan, Iran), the higher prevalence of ORT in this study is possible.…”

“…ORT is a Gram negative, pleomorphic, rod shaped and non-motile bacterial pathogen mostly known to cause respiratory tract infections such as airsacculitis and pneumonia in birds such as turkeys and other poultry all over the world (Ghanbarpour and Salehi, 2009;Schuijffel et al, 2005). This microorganism is a new poultry pathogen associated with respiratory disease, decreased growth and increased mortality in chickens and turkeys (Chin and Droual, 1997;Hafez, 1996).…”

“…The symptoms of a disease in turkey and other poultry are respiratory problems, together with purulent pneumonia, airsacculitis, severe growth retardation and rapidly increasing mortality, especially in chicken (Empel et al, 1997). Respiratory tract infection is a major problem within the poultry industry and is accompanied by a major economic loss due to retarded growth, increased medication costs, increased loss rates and poor production (Ghanbarpour and Salehi, 2009;Uriarte et al, 2010). ORT has been isolated from chicken, chukar partridge, duck, goose, guinea fowl, gull, ostrich, partridge, pheasant, pigeon, quail, rooks and turkey (Allymehr, 2006).…”

<i>Ornithobacterium rhinotracheale</i> Infection in Poultry: an Updated Review

“…However, O. rhinotracheale excretion and its antibody response may also be affected by a number of factors such as antibiotic therapy and vaccination (Hafez, 2002). ELISAs, serum plate agglutination tests, and a dot-immunobinding assay have been reported for the detection of O. rhinotracheale antibodies (Back et al, 1998;van Empel and Hafez, 1999;Sakai et al, 2000;Erganiş et al, 2002;Hafez, 2002;Canal et al, 2003a;Allymehr, 2006;Eroksuz et al, 2006;Chansiripornchai et al, 2007;Ghanbarpour and Salehi, 2009;Uriarte et al, 2010;Walters et al, 2010).…”

“…ELISAs have been developed using different serotypes and extracted antigens of O. rhinotracheale , and both self-made and commercially available ELISAs (Biochek, Inc., USA, and IDEXX Laboratories, Inc., USA) are used to detect antibodies against most O. rhinotracheale serotypes (Hafez, 2002 (Canal et al, 2003a;Allymehr, 2006;Chansiripornchai et al, 2007;Ghanbarpour and Salehi, 2009;Uriarte et al, 2010).…”