Seroconversion and Abundance of IgG Antibodies against S1-RBD of SARS-CoV-2 and Neutralizing Activity in the Chilean Population

González-Stegmaier, Roxana; Cereceda, Karina; Briones, José Luis Teruel; Beltrán-Pavez, Carolina; Oyarzún-Arrau, Aarón; Riquelme-Barrios, Sebastián; Selman, Carolina; Yarad, F.; Mahave, Mauricio; Caglevic, Christian; Morales, Rogerio Rizo; Aguirre, Adam; Valiente-Echeverría, Fernando; Soto-Rifo, Ricardo; Marsiglia, H; Gazitúa, Raimundo; Villarroel‐Espíndola, Franz

doi:10.1155/2021/6680337

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…The seroconversion of anti-S was observed in all HCWs after 30 days of the RT-PCR test. Anti-S concentration was heterogeneous among HCWs (range 23.8 to 250, median 179.8), as has been widely described [ 9 , 10 ]. The antibody was relatively stable until 90 days after the RT-PCR test.…”

Section: Discussionmentioning

confidence: 80%

Co-Occurrence of SARS-CoV-2 Infection and Inactivated SARS-CoV-2 Vaccination among Healthcare Workers

Cucunawangsih

Wijaya

Lugito

et al. 2021

Case Reports in Infectious Diseases

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The presented cases describe the concurrent SARS-CoV-2 infection and inactivated SARS-CoV-2 vaccination among eight healthcare workers (HCWs). These cases highlighted the importance of broad hospital screening during the COVID-19 vaccination campaign. Further study regarding the durability of antibody response induced by infection and first-dose vaccination is required to determine the appropriate time for giving a second dose of inactivated SARS-CoV-2 vaccine among these cases.

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Section: Discussionmentioning

confidence: 80%

Co-Occurrence of SARS-CoV-2 Infection and Inactivated SARS-CoV-2 Vaccination among Healthcare Workers

Cucunawangsih

Wijaya

Lugito

et al. 2021

Case Reports in Infectious Diseases

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“…Looking at these limitations, an attempt has been made to develop a simple, specific and sensitive ELISA Kit for the detection of the IgG against S1-RBD of SARS-CoV-2. The antibodies mainly IgG produced against the S1-RBD have shown the virus-neutralizing capacity ( González-Stegmaier et al, 2021 ). The role of S1 RBD has also been identified in the viral entry into the host cells and it is less conserved in the SARS-CoV family ( Bastos et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples

Kumari¹,

Raina²,

Chandra³

et al. 2022

Journal of Virological Methods

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Since the pandemic occurred due to the emergence of SARS-CoV-2, there has always been a demand for a simple and sensitive diagnostic kit for detection of SARS-Cov-2 infection. In January 2020, WHO approved the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) for detecting the presence of Covid-19 genetic material in individuals. Till date many diagnostic kits have arrived in the market for quantification of SARS-CoV-2 antibodies. In spite of being the gold standard method of Covid-19 detection, there are some drawbacks associated with RT-PCR which leads to false-negative results. Hence, in order to fulfil the need for an antibody testing kit for evaluating seroconversion and immunity acquisition in the population, an efficient, highly specific and sensitive assay, Chimera Soochak, an enzyme-linked immunoassay (ELISA) Kit has been developed. It works on the principle of detecting IgG antibodies developed specifically against the S1-RBD by employing a recombinant strain of S1-RBD produced in the HEK293 cell line. The developed kit was validated using different modes and methods to attain the utmost confidence on the samples collected from patients. The validation methodology included, validation with known samples, blind study, third-party validation, validation using WHO Reference Panel and comparison with FDA approved Surrogate virus neutralization kit. The kit was found successful in detecting IgG against the S1-RBD of SARS-CoV-2. The kit had been validated on multiple parameters. A total of 900 samples had been tested by using this kit and it has exhibited the sensitivity, specificity and accuracy for all the above-mentioned parameters.

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“…The coronavirus disease outbreak in late 2019 (COVID-19) has emerged as a worldwide pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [ 1 ]. The virus outbreak began in December 2019 in Wuhan, China, and spread largely through droplets and surface contact routes [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of SARS-CoV-2 immunoglobulin G antibody during COVID-19 pandemic in Fayoum District, Egypt: a community-based pilot survey

Bakr¹,

Ezzat²,

Salem³

et al. 2023

Pan Afr Med J

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Introduction controlling the worldwide pandemic, coronavirus disease (COVID-19), could be impossible due to the hesitancy about the available vaccines and the difficulty to implement strict restrictions. Little information is available about herd immunity in the highly vulnerable region of North East Africa, Egypt. The objective of this study was to assess the seroprevalence of SARS-CoV-2 during the pandemic in one of the highly vulnerable populations in Egypt, the Fayoum district of Fayoum Governorate. Additionally, to assess the predictive value of symptoms and other associated risk factors towards a positive COVID-19 test. Methods in this cross-sectional community-based pilot study, immunoglobulin G (IgG) antibodies that are specific for the SARS-CoV-2 spike (S1-RBD) protein were tested during the period from February 2021 to July 2021. Results out of 155 participants, 60.6% were SARS-CoV-2 seropositive. Out of symptomatic and asymptomatic individuals, 76.5% and 56.2% were seropositive, respectively. Surprisingly, only one individual had received the COVID-19 vaccine. Previous history of COVID-19; such as symptoms and gender are statistically significant predictors of high seroconversion independent of age, comorbidities, and level of education. Conclusion this study which disclosed unexpectedly high SARS-CoV-2 seroconversion among the Egyptians, might provide a clear insight into COVID-19 transmission patterns and state of immunity. Further study with a larger sample size on a large scale is required to represent the whole local population.

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Seroconversion and Abundance of IgG Antibodies against S1-RBD of SARS-CoV-2 and Neutralizing Activity in the Chilean Population

Cited by 5 publications

References 32 publications

Co-Occurrence of SARS-CoV-2 Infection and Inactivated SARS-CoV-2 Vaccination among Healthcare Workers

Co-Occurrence of SARS-CoV-2 Infection and Inactivated SARS-CoV-2 Vaccination among Healthcare Workers

Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples

Seroprevalence of SARS-CoV-2 immunoglobulin G antibody during COVID-19 pandemic in Fayoum District, Egypt: a community-based pilot survey

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