Serodetection of Ehrlichia canis infection in dogs from Ludhiana district of Punjab, India

Singla, L. D.; Singh, Harkirat; Kaur, Paramjit; Singh, Namita; Singh, Niraj Kumar; Juyal, P. D.

doi:10.1007/s12639-011-0055-2

Cited by 13 publications

(12 citation statements)

References 13 publications

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“…E. canis is more prevalent in tropical and subtropical areas in India, Asia, Central and South America and Africa (SINGLA et al, 2011;BABA et al, 2012;RODRIGUEZ-VIVAS et al, 2005;AGUIAR et al, 2007a;MATJILA et al, 2008). In South America, it has been reported in Brazil (VIEIRA et al, 2011), Venezuela (UNVER et al, 2001, Chile (LÓPEZ et al, 2012) and Peru (VINASCO et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Costa

Labruna

et al. 2015

Rev. Bras. Parasitol. Vet.

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This study evaluated exposure and infection by tick-borne agents (Babesia vogeli, Ehrlichia canis and Rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of Chapadinha, state of Maranhão, northeastern Brazil, using molecular and serological methods. Overall, 16.1% of the sampled dogs (52/322) were seroreactive to B. vogeli, with endpoint titers ranging from 40 to 640. For E. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 to 163,840. Antibodies reactive to at least one of the five species of Rickettsia were detected in 18.9% of the dogs (61/322), with endpoint titers ranging from 64 to 4,096. High endpoint titers were observed for Rickettsia amblyommii. Three (0.9%) and nine (2.8%) canine blood samples were PCR-positive for Babesia spp. and E. canis. The ticks collected from urban dogs were all Rhipicephalus sanguineus sensu lato, whereas the rural dogs were infested by R. sanguineus s.l, Amblyomma cajennense sensu lato and Amblyomma ovale. One A. ovale tick was found to be infected by Rickettsia bellii. This study provides an epidemiological background for controlling and preventing canine tick-borne diseases in a neglected region of Brazil.Keywords: Ticks, tick-borne pathogens, hemoparasites. ResumoEste estudo avaliou por métodos sorológicos e moleculares a exposição e infecção por agentes transmitidos por carrapatos (Babesia vogeli, Ehrlichia canis, and Rickettsia spp.)

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Section: Introductionmentioning

confidence: 99%

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Costa

Labruna

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…B. gibsoni (Table 1). The limitations of microscopy in detecting E. canis morulae has also been reported by prior studies (Mylonakis et al, 2004;Singla et al, 2011;Abd Rani et al, 2011) and therefore make it a less sensitive technique for confirmatory diagnosis of canine ehrlichiosis. Moreover, sensitivity of E. canis detection by microscopy is low due to the fact that in chronic cases number of infected cells in peripheral circulation is very less to be detected by microscopic examination.…”

Section: Discussionmentioning

confidence: 83%

“…Prevalence studies of CME from Northern India were mainly concentrated from Ludhiana city in Punjab over the last few years in pet dogs and non-descript local dogs that were bought to the clinic. Singla et al (2011) reported a high seroprevalence of 61% in pet dogs when tested by commercial serological kit. The same group in their molecular studies through nested PCR in 2016 in the same city detected meagre 0.39% prevalence of E. canis DNA in pet dogs.…”

Section: Discussionmentioning

confidence: 99%

“…Till date, studies on CME from India have been carried out in small geographical locations (cities/towns/small states) and that too, they have been carried out either on pet or on stray dogs and somehow, majority of these studies fail to address the clinical and diagnostic challenges being presently posed by the disease (Juyal et al, 1994;Samaradni et al, 2003;Lakshmanan et al, 2006;Wise and Tarlinton, 2012;Abd Rani et al, 2011;Singla et al, 2011;Milanjeet et al, 2014;Bhadesiya and Raval, 2015;Singla et al, 2016;Kottadamane et al, 2016Kottadamane et al, , 2017. Besides these lacunae, these prevalence studies were also solely based on either commercial serology kit or on molecular based techniques and thus failed to analyse the practical relevance of both the methodologies in existing field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Mittal

Kundu

Chakravarti

et al. 2017

Preventive Veterinary Medicine

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Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking. Study of CME among these dog populations was thus carried out, encompassing clinical aspects and different diagnostic methodologies viz., microscopy, serology and molecular biology. During the two-year study period, clinical specimens from 225 cases suspected of canine ehrlichiosis were examined for clinical pathology and presence of the haemoparasites. Overall prevalence of ehrlichiosis by microscopic examination, commercial dot-ELISA kit and nested PCR assay was estimated to be 1.3%, 19.1% and 5.8%, respectively, which were found to be statistically significant by McNemar Chi squared test (p<0.05). It was also observed that possibly due to widespread use of doxycycline therapy in field, CME presently does not remain a potential threat which it uses to pose earlier. However, concurrent infections of E. canis and Babesia gibsoni were found to be mostly fatal. Keeping in view of high number of apparently healthy dogs (24) out of total positive cases (46) observed during the study, it is recommended that prevalence studies on CME should also involve screening of apparently healthy dogs. Phylogenetic analysis carried on partial sequencing of 16S rRNA of E. canis strains revealed that all of the Indian strains clustered in a single clade with other E. canis species from India and rest of the world. Molecular divergence was observed among the sequences of Brazilian and American isolates which were also included in the present study. These findings have thus opened a new paradigm for planning of pragmatic control strategies against CME.

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“…Strays may also include the quasi-owned animals that are cared for or considered to belong to the "neighbourhood" (Childs et al, 1998). Stray dogs pose several public health risks and concerns to humans, such as rabies (Reyers et al, 1998;Crosby, 2011), toxocariasis and ancylostomiasis (Croese et al, 1994;Prociv and Croese 1996;Singla et al, 2005;Chen et al, 2012), hydatidosis (Buishi et al, 2006), acquired epilepsy due to neurocysticercosis (Sharma et al, 2011), leishmaniasis, borreliosis, bartonellosis, ehrlichiosis, rickettsiosis (Day, 2011;Singla et al, 2011), and schistosomiasis (Hackett and Lappin, 2003).…”

Section: Introductionmentioning

confidence: 99%

Assessment of fecal parasitism for decision making in continued deworming of slum stray dogs in Nairobi areas of Kenya

Makau¹,

Mulei²,

Mutembei³

et al. 2015

J. Vet. Med. Anim. Health

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The objective of this study was to establish the density of infestation of slum dogs with gastrointestinal helminths in locations where vaccination and deworming campaigns had been carried out for a period of 3 years. A total of 150 dogs were randomly sampled from a total of 300 animals that were attended to in a rabies control campaign in Nairobi slum areas between the months of July and September, 2011. Eggs per gram (EPG) and worm identification were done from the fecal samples of the animals included in the study and the prevalence on age, sex and breed were also determined. The EPG were generally high for both hookworms and ascarids with a range of 1083 to 1105 and 515 to 637, respectively. The larvae of the most prevalent species identified were Ancylostoma caninum and Toxocara canis. There was no statistical significant difference (P=0.9) between EPG counts in males and females at p<0.05. The prevalence was high among the puppies at 58.8% (44/75) as compared to the adult dogs at 38.3% (29/75) when P = 0.01. From the findings of this study, it was concluded that the density of infestation of stray slum dogs with gastrointestinal worms was high and therefore there is need to address routine and strategic deworming.

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Serodetection of Ehrlichia canis infection in dogs from Ludhiana district of Punjab, India

Cited by 13 publications

References 13 publications

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Assessment of fecal parasitism for decision making in continued deworming of slum stray dogs in Nairobi areas of Kenya

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