Serodiagnosis of a viral haemorrhagic septicaemia virus infection

Kang, Bongjo; Kim, K J; Kim, T J

doi:10.1111/jfd.12233

Cited by 3 publications

(7 citation statements)

References 13 publications

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“…The rec-GP from the E. coli was prepared as described previously (Kang et al 2015). Briefly, whole GP gene was cloned into a pRSET plasmid (Invitrogen, Carlsbad, CA, USA), transformed into BL21(DE3)pLysS (Invitrogen) for the expression, and then purified using a Probond ™ purification system (Invitrogen) according to the manufacturer's instructions.…”

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

“…A total of 25 6-week-old ICR female mice (Orient Bio Inc, Seongnam, Republic of Korea) were divided into five groups as shown in Table 1. The mice were primed with PBS (Group 1) or 50 μg of rec-GP from the E. coli (Group 2) (Kang et al 2015) with Freund's complete adjuvant. For oral boosting, the mice of Groups 1-2 and 2-1 received a tobacco-derived CTB-fused rec-VHSV-GP-1, while Groups 1-3 and 2-2 received a CTB-fused rec-VHSV-GP2 (Tien et al 2017) every Monday for a total of four times.…”

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

“…One day before each immunisation, the sequential blood samples (2 nd to 5 th ) were collected. The presence of an antibody against VHSV GP was determined using ELISA (an enzyme-linked immunosorbent assay) as described (Kang et al 2015). Briefly, the rec-GP from the E. coli was used as antigen.…”

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

“…A total of 85 apparently healthy (without clinical signs) olive flounders, Paralichthys olivaceous (weight: 40-50 g, length: 15-17 cm, approximately two months after moving 7 cm-long fingerlings into culture farms from a nursery) were obtained https://doi.org/10.17221/26/2019-VETMED from the culture farms and kept in separate tanks supplied with ample aeration and artificial sea water at 22 °C (± 1 °C). After acclimatisation for two weeks, five fish were selected randomly and the presence (infection) of VHSV in the fish's kidney was tested using the VHSV-specific RT-PCR method (Kang et al 2015).…”

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

See 3 more Smart Citations

Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco

Kim¹,

Kang²,

Si³

et al. 2019

Vet. Med. - Czech

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The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically, especially in the primed groups (P < 0.0001) in both the mouse and fish. After the viral challenge under low water temperature culture conditions (below 18 °C), the immunised fish were protected successfully against the challenge, showing a significantly lower mortality rate (P < 0.05). This result suggests that this plant-based immunisation system could induce an effective immune response. It could be used as a candidate to develop an oral vaccine for fish.

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Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

Section: Recombinant Proteins From Tobacco and E Colimentioning

confidence: 99%

See 2 more Smart Citations

Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco

Kim¹,

Kang²,

Si³

et al. 2019

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…The most commonly used strategy for setting a cutoff point is the use of the mean OD value for a free population plus 1, 2, or 3 standard deviations (Breuil and Romestand 1999;Encinas et al 2011). Other strategies include establishment of arbitrary cutoff points at set values, such as OD = 0.1 or OD = 0.05 (Mushiake et al 1992;Kim et al 2008); use of a set ratio between sample and internal controls (Kang et al 2015); and receiver operating characteristic (ROC) analysis based on positive and negative reference populations (Wilson et al 2014).…”

Section: Test Evaluationmentioning

confidence: 99%

Serology in Finfish for Diagnosis, Surveillance, and Research: A Systematic Review

et al. 2017

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Historically, serological tests for finfish diseases have been underused when compared with their use in terrestrial animal health. For years the nonspecific immune response in fish was judged to make serology unreliable and inferior to the direct measurement of agent analytes. We conducted a systematic review of peer-reviewed publications that reported on the development, validation, or application of serological tests for finfish diseases. A total of 168 articles met the screening criteria; most of them were focused on salmonid pathogens (e.g., Aeromonas spp. and viral hemorrhagic septicemia virus). Before the 1980s, most publications reported the use of agglutination tests, but our review indicates that enzyme-linked immunosorbent assay (ELISA) has more recently become the dominant serological test. The main application of serological tests has been in the assessment of vaccine efficacy, with few applications for surveillance or demonstration of freedom from disease, despite the advantages of serological tests over direct detection at the population level. Nonlethal sampling, low cost, and postinfection persistence of antibodies make serological assays the test of choice in surveillance, especially of valuable broodstock. However, their adoption has been constrained by poor characterization and validation. The number of publications in our review reporting diagnostic sensitivity and specificity of serological tests in finfish was small (n = 7). Foreseeing a wider use of serological tests in the future for diagnostic end purposes, we offer recommendations for mitigating deficiencies in the development and evaluation of serological tests, including optimization, control of nonspecific reactions, informed cutoff points, diagnostic accuracy, and serological baseline studies. Achieving these goals will facilitate greater international recognition of serological testing in programs supporting aquatic animal health. Received March 21, 2016; accepted September 24, 2016.

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Viral hemorrhagic septicemia virus glycoprotein production in tobacco

Tien

Kim

2017

Protein Expression and Purification

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Serodiagnosis of a viral haemorrhagic septicaemia virus infection

Cited by 3 publications

References 13 publications

Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco

Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco

Serology in Finfish for Diagnosis, Surveillance, and Research: A Systematic Review

Viral hemorrhagic septicemia virus glycoprotein production in tobacco

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