Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Verdida, Rodolfo A.; Hara, Olgga A.; Xuan, Xuenan; Fukumoto, Shinya; Igarashi, Ikuo; Shou-fa, Zhang; Dong, Junyan; Inokuma, Hisashi; Kabeya, Hidenori; Sato, Yukita; Moritomo, Tadaaki; Maruyama, Soichi; Claveria, Florencia G.; Nagasawa, Hideyuki

doi:10.1292/jvms.66.1517

Cited by 24 publications

(28 citation statements)

References 19 publications

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“…Both the PCR and ELISA with GSTP50t tests used in this study have been reported to be sensitive and specific methods for the detection of B. gibsoni infection in dogs [8,20]. Using these tests, we detected B. gibsoni infection in 35 dogs from 8 prefectures in the eastern part of Japan.…”

Section: Discussionmentioning

confidence: 85%

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Epidemiological Survey of Babesia gibsoni Infection in Dogs in Eastern Japan

Miyama

Sakata²,

Shimada

et al. 2005

J. Vet. Med. Sci.

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ABSTRACT. To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twentytwo of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than that of B. gibsoni-negative dogs.

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Section: Discussionmentioning

confidence: 85%

“…ELISA: ELISA with GST-P50t was essentially carried out according to the protocol of Verdida et al [20]. Briefly, 96-well microplates were coated with the antigens, GST-P50t and GST (negative control), at a concentration of 250 ng per well.…”

Section: Samplingmentioning

confidence: 99%

Epidemiological Survey of Babesia gibsoni Infection in Dogs in Eastern Japan

Miyama

Sakata²,

Shimada

et al. 2005

J. Vet. Med. Sci.

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“…Their homology with Bc28 suggests an erythrocyte binding ability, although no direct evidence has been reported. The use of the p50 recombinant protein in ELISAs revealed the presence of antibodies against this protein in dogs from the field (38). In addition, antibodies against recombinant p50 have been shown to inhibit the parasite development in a mouse model (39), and dog vaccination with both DNA coding for p50 and recombinant virus expressing p50 induces a weak protection against B. gibsoni parasites (40).…”

Section: Discussionmentioning

confidence: 99%

Structural and Functional Characterization of Bc28.1, Major Erythrocyte-binding Protein from Babesia canis Merozoite Surface

Yang

Murciano

Moubri

et al. 2012

Journal of Biological Chemistry

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Babesiosis (formerly known as piroplasmosis) is a tick-borne disease caused by the intraerythrocytic development of protozoa parasites from the genus Babesia. Like Plasmodium falciparum, the agent of malaria, or Toxoplasma gondii, responsible for human toxoplasmosis, Babesia belongs to the Apicomplexa family. Babesia canis is the agent of the canine babesiosis in Europe. Clinical manifestations of this disease range from mild to severe and possibly lead to death by multiple organ failure. The identification and characterization of parasite surface proteins represent major goals, both for the understanding of the Apicomplexa invasion process and for the vaccine potential of such antigens. Indeed, we have already shown that Bd37, the major antigenic adhesion protein from Babesia divergens, the agent of bovine babesiosis, was able to induce complete protection against various parasite strains. The major merozoite surface antigens of Babesia canis have been described as a 28-kDa membrane protein family, anchored at the surface of the merozoite. Here, we demonstrate that Bc28.1, a major member of this multigenic family, is expressed at high levels at the surface of the merozoite. This protein is also found in the parasite in vitro culture supernatants, which are the basis of effective vaccines against canine babesiosis. We defined the erythrocyte binding function of Bc28.1 and determined its high resolution solution structure using NMR spectroscopy. Surprisingly, although these proteins are thought to play a similar role in the adhesion process, the structure of Bc28.1 from B. canis appears unrelated to the previously published structure of Bd37 from B. divergens. Site-directed mutagenesis experiments also suggest that the mechanism of the interaction with the erythrocyte membrane could be different for the two proteins. The resolution of the structure of Bc28 represents a milestone for the characterization of the parasite erythrocyte binding and its interaction with the host immune system.The phylum Apicomplexa, a large group of single-celled eukaryotic intracellular parasites, includes some of the most important pathogenic parasites of humans and animals, the deadliest of which is the malaria parasite Plasmodium falciparum, responsible for one million human deaths per year and with almost half of the human population at risk of contracting malaria. No vaccine currently exists against P. falciparum, and parasites are becoming increasingly resistant to pharmaceuticals. This is also true for other pathogenic Apicomplexa, such as Toxoplasma, which, together with Plasmodium, challenge global human health improvement programs, and coccidiosis and babesiosis are detrimental for agri-business in both industrialized and developing countries. Babesiosis (formerly known as piroplasmosis) is a tick-borne disease caused by the intraerythrocytic development of an Apicomplexa from the Babesia genus. Hemolytic anemia due to parasite development leads to major symptoms, such as hemoglobinury, fever, asthenia, and renal failure. Amo...

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“…Serological tests like, indirect fluorescent antibody tests (IFAT), Indirectenzyme linked immunosorbent test are considered to be highly sensitive (Kaur et al, 2016), but only moderately specific because of antigenic cross-reactions to other Babesia spp (Yamane et al, 1993). The recent development of recombinant ELISA assays has improved test specificity (Verdida et al, 2004). Several PCR methods have been reported to diagnose B. gibsoni infection with high sensitivity and specificity (Birkenheuer et al, 2003;Inokuma et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of different diagnostic tests for B. gibsoni in dogs

Kushwaha

Mondal

Mahapatra

2018

IJAR

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Babesia gibsoni is the common cause of canine babesiosis in India. Its diagnosis by conventional diagnostic methods result in false negative condition, particularly in those cases in which parasitemia is low. Therefore the present study employed IFAT and PCR along with microscopic examination to overcome this situation. Microscopic examination of Giemsastained blood smears revealed presence of small pear-shaped, oval or signet ring shaped B. gibsoni within the erythrocytes of 1.34 % dogs. IFAT and PCR detected a total of 1.80 % and 1.82 % dogs positive for B. gibsoni, respectively. The sensitivity and specificity of IFAT was 93.10% and 88.89%, respectively whereas, sensitivity and specificity of microscopic examination was 71.26% and 95.56%, respectively. The agreement of IFAT and microscopic examination with PCR was 82.30% and 60.73%, respectively. The agreement between IFAT and microscopy was 60.99%. Therefore, IFAT is considered to be highly sensitive test for diagnosing B. gibsoni in subclinically or chronically infected dogs with significantly low level of parasitemia.

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Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Cited by 24 publications

References 19 publications

Epidemiological Survey of Babesia gibsoni Infection in Dogs in Eastern Japan

Epidemiological Survey of Babesia gibsoni Infection in Dogs in Eastern Japan

Structural and Functional Characterization of Bc28.1, Major Erythrocyte-binding Protein from Babesia canis Merozoite Surface

Comparative evaluation of different diagnostic tests for B. gibsoni in dogs

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