Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus.

Chiba, Joe; Ohba, Hiroyoshi; Matsuura, Yoshiharu; Watanabe, Yoji; Katayama, Tohru; Kikuchi, Shu; Saito, Izumu; Miyamura, Tatsuo

doi:10.1073/pnas.88.11.4641

Cited by 117 publications

(57 citation statements)

References 29 publications

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“…Patients were from St. Marianna Hospital, Kawasaki, Kanagawa; Tokyo National Chest Hospital, Kiyose, Tokyo; and Shinshu University School of Medicine, Nagano. 12,13 Informed consent was obtained from all patients, and the study had the approval of the Ethical Committees of the hospitals. To study the natural history of acute non-A non-B hepatitis, serial sera samples had been collected over many years and maintained at Ϫ20°C.…”

Section: Methodsmentioning

confidence: 99%

“…11 This assay is used as a surrogate test to estimate the level of HCV-neutralizing (NOB) antibodies. Here we report on the serum antibody responses to HCV structural proteins in a rare group of patients who spontaneously resolved chronic hepatitis C. 12,13 We have found that the appearance and maintenance of high serum titers of NOB antibodies in the course of chronic hepatitis C is accompanied by virus clearance and clinical resolution of liver disease.…”

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High titers of antibodies inhibiting the binding of envelope to human cells correlate with natural resolution of chronic hepatitis C

et al. 1998

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

High titers of antibodies inhibiting the binding of envelope to human cells correlate with natural resolution of chronic hepatitis C

et al. 1998

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“…Each well of the ELISA plates (ICN-Flow Laboratories) was coated with 50 p.1 of the antigen solution for 16 h at 4 °C. Human sera were analysed for anti-E2 Ab by ELISA using the coated antigens as described (Chiba et al, 1991). Western blotting.…”

Section: Methodsmentioning

confidence: 99%

Establishment of a cell line constitutively expressing E2 glycoprotein of hepatitis C virus and humoral response of hepatitis C patients to the expressed protein

Harada

Suzuki

Ando

et al. 1995

Journal of General Virology

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A Chinese hamster ovary cell line was established which abundantly expresses the second envelope protein (E2) of hepatitis C virus under the control of an exogenous promoter. The expressed E2 protein was found to be a glycoprotein of 58 kDa by immunoprecipitation with sera from patients that had chronic hepatitis C. Using this cell line as antigen in immunofluorescence tests, as high as 93 % of patients with non-A non-B hepatitis had antibodies against E2 protein. In Western blots using SDS-denatured E2 protein, however, the detectability of the antibody was drastically reduced to 30 %. Immunoprecipitation assays and ELISA, using both native and denatured E2 protein, revealed that antibodies to E2 protein were present in most of the chronic hepatitis C patients and that they reacted only to the native forms.

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“…Chiba et al (1991) and Matsuura et al (1992) have reported that p22 and gp35 produced in insect cells infected with recombinant 0001-0959 © 1992 SGM baculovirus carrying cDNAs of the corresponding genome regions were detected by sera from NANBH patients. Thus, it is suggested that p22 and gp35 are the mature viral proteins existing in cells of patients infected with HCV.…”

Section: Introductionmentioning

confidence: 99%

Expression and characterization of glycoprotein gp35 of hepatitis C virus using recombinant vaccinia virus

Kohara

Tsukiyama-Kohara

Maki³

et al. 1992

Journal of General Virology

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Complementary DNA clones corresponding to one of the putative structural regions of the hepatitis C virus (HCV) genome were obtained from sera of non-A non-B hepatitis patients. The putative envelope gene was expressed by using a recombinant vaccinia virus carrying this region of the HCV genome. In cells infected with the recombinant vaccinia virus, a glycosylated protein with an Mr of about 35K (gp35) was specifically detected by convalescent sera from hepatitis C patients. The sera from rabbits immunized with this recombinant vaccinia virus reacted to the gp35 produced in insect cells and also to gp35 which was translated in vitro in the glycosylated and processed form. The gp35 was used to detect antibodies in sera of only 7 to 23 % of HCV patients at various stages of HCV disease. These results suggest that the gp35 of HCV may not have high antigenicity in humans.

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Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus.

Cited by 117 publications

References 29 publications

High titers of antibodies inhibiting the binding of envelope to human cells correlate with natural resolution of chronic hepatitis C

High titers of antibodies inhibiting the binding of envelope to human cells correlate with natural resolution of chronic hepatitis C

Establishment of a cell line constitutively expressing E2 glycoprotein of hepatitis C virus and humoral response of hepatitis C patients to the expressed protein

Expression and characterization of glycoprotein gp35 of hepatitis C virus using recombinant vaccinia virus

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