Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1

Kimbita, Elikira; Xu, Xuan; Huang, Xiaohong; Miyazawa, Takayuki; Fukumoto, Shinya; Mishima, Masayuki; Suzuki, Hiroshi; Sugimoto, Chihiro; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; Igarashi, Ikuo

doi:10.1016/s0304-4017(01)00522-2

Cited by 67 publications

(42 citation statements)

References 20 publications

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“…The recombinant protein was expressed as the fusion protein of the glutathione S-transferase (GST) protein in the Escherichia coli DH5␣ strain according to the manufacturer's instructions (Promega) and designated GST-P50. The purification of GST-P50 was performed as described previously (13). The ELISA with GST-P50 was carried out according to a method described earlier (6,25).…”

Section: Methodsmentioning

confidence: 99%

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

Fukumoto

Kadota

et al. 2003

Clin Vaccine Immunol

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Section: Methodsmentioning

confidence: 99%

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

Fukumoto

Kadota

et al. 2003

Clin Vaccine Immunol

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“…This approach has been successfully used previously for the expression of other parasite antigens [15,18,20,30].…”

Section: Introductionmentioning

confidence: 99%

Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

et al. 2007

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-The mite Sarcoptes scabiei causes sarcoptic mange (or scabies), a disease of considerable human and veterinary significance. An S. scabiei cDNA clone of about 2 kb was isolated from a S. scabiei var. hominis expression library by immunological screening using blood serum from a naturally infected chamois (Rupicapra rupicapra). The nucleotide sequence of the identified cDNA contains an open reading frame of 1930 bp that encodes a 642 amino acid polypeptide. This polypeptide shows tandem repeats of a glycine-serine rich 20 residue sequence followed by a unique C-terminal glutamate rich 54 residue sequence. The cDNA or the deduced polypeptide did not show significant similarities to any of the sequences in the databases. A carboxyl-terminal fragment of this polypeptide (residues 380 to 642) was efficiently expressed in Escherichia coli as a fusion with Glutathione S-transferase and then was used to produce a specific antiserum. The antigen encoded by the cDNA was located at the integument of the mite's epidermis and the cavities surrounding its vital organs. Western blot analysis of mite extracts using the specific antiserum against the recombinant protein identified antigens larger that 60 kDa indicating that the isolated cDNA did not contain the full ORF. Moreover, we designed a diagnostic assay based on the carboxyl-terminal fragment of the antigen for the identification of infected animals.Sarcoptes scabiei / cDNA library / immunolocalisation / sarcoptic mange diagnosis / ELISA

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“…A truncated SAG2 without the highly hydrophobic signal peptide and C terminus was thus cloned and expressed to improve the yield of the soluble recombinant antigen. Briefly, a 438-bp DNA fragment encoding the truncated SAG2 was amplified by PCR with two oligonucleotide primers, 5Ј-ACGAATTCGTCCACCACCGAGACG-3Ј and 5Ј-ACGAATTCTTACTTGCCCGTGAGA-3Ј (10), and template DNA extracted from tachyzoites of T. gondii strain RH (9). Then, the PCR product was inserted into an EcoRI site of pGEX-4T-3.…”

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confidence: 99%

Rapid Immunochromatographic Test Using Recombinant SAG2 for Detection of Antibodies against Toxoplasma gondii in Cats

Huang

Hirata

et al. 2004

J Clin Microbiol

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Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1

Cited by 67 publications

References 20 publications

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

Rapid Immunochromatographic Test Using Recombinant SAG2 for Detection of Antibodies against Toxoplasma gondii in Cats

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