Seroepidemiological study of toxoplasmosis in hemodialysis patients of Ahvaz, Southwest of Iran

Kazemi, Forough; Fallahizadeh, Somayeh; Feizhadad, Mohammad Hosein

doi:10.22631/ijbmph.2018.55340

Cited by 3 publications

(3 citation statements)

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“…When the immune system is compromised, acute or active infections, such as encephalitis, can lead to life-threatening complications. The study of Forough Kazemi, [20] , Al-Saadawi and Alkhaled [21] , Ayser and Abdul-Aziz [22] Bayani,M [23] ,Al-Dulaimi [24], Sharif Maraghi [25], recorded similar results of our current study. On the other hand, there are studies that differ with it Seyyedpour [26].…”

Section: Discussionsupporting

confidence: 89%

Impact of Toxoplasmosis in Im-mune Respons in Hemodialysis Patients

Salem¹,

Shaker²,

Mohammed³

2022

DJM

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Background:Toxoplasma gondii is the most common infection of protozoa, affecting a wide range of hosts. Toxoplasmosis is usually asymptomatic in immu-nocompetent persons, but in immunocompromised persons (ex:patients on dialysis) significant problems can ensure, and may progress to a life-threatening infection. Objective: To investigate the seroprevalence of anti-Toxoplasma anti-bodies in patients undergoing hemodialysis in Baquba / Iraq . Patients and Methods: A group of blood samples, consisting of 75 dial-ysis patients and 50 healthy controls, were examined for the detection of anti-toxoplasma antibodies in Baquba Governorate from November 2020 to April 2021 using enzyme-linked immunosorbent assay (ELISA) for IgG and IgM. Results: The total numbers of the 125 samples were examined, 32 (44.0%) and 2 (4.0%) were IgG-positive for both patients and healthy individual respectively, and the results of the current study showed only 1 (1.33%) was seropositive IgM for patient group, while no seroposi-tive healthy subjects was found for IgM . The results showed that the percentage of seropositivity was higher in 17 males (40.47%) than in females 15 (45.45%) in the group of patients, but the differences were not statistically significant . As for the age groups, a positive increase was observed in the age group 11-30 years when comparing patients and healthy people, as the percentage reached 5 (29.41%). It was also ob-served that the highest seropositivity in the education levels was in the group of patients at the educational level 6-12 (45.45%) than in the healthy controls group, but the differences were not statistically signifi-cant between education levels. Patients living in urban areas had signifi-cantly higher serum (P = 0.0041) than patients in rural areas, and the odds ratio was 22,000. And those who had contact with cats showed a significant increase in seropositivity p = 0.0466 and odds ratio was 20.5789. Conclusion: Dialysis patients are at risk for toxoplasmosis and should be screened on a regular basis to prevent the disease from spreading dur-ing hemodialysis. Keywords: Toxoplasma gondii, Hemodialysis, Antibodies ,IgG

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Section: Discussionsupporting

confidence: 89%

Impact of Toxoplasmosis in Im-mune Respons in Hemodialysis Patients

Salem¹,

Shaker²,

Mohammed³

2022

DJM

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“…Toxoplasma gondii (T. gondii) is a protozoan that results in a zoonotic disease known toxoplasmosis [1]. Sero-epidemiological studies have shown that the parasite antibodies are in serum of one-third of the world adult population [2]. The clinical signs of this disease lead to a substantial disease burden in humans worldwide, including ocular, cerebral, and congenital toxoplasmosis [1,3].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, this parasite can result in clinical manifestations in animal hosts of T. gondii, leading to lesser welfare for the affected hosts as well as major losses in livestock industry [1]. However, signs in people with healthy immune system are transient and mild which include swollen lymph and mild fever glands [2].…”

Section: Introductionmentioning

confidence: 99%

Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?

et al. 2022

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Introduction IgG antibodies against T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-linked immunosorbent assay (ELISA) methods for detection of T. gondii IgG antibody. Methods Immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against T. gondii were measured by the ELISA method in 81 participants. In addition, detection of acute and chronic toxoplasmosis was performed via the ELISA IgG avidity. The set-up of iPCR was carried out and then, serum IgG of subjects were detected using the iPCR method. Results Of 81 samples, 4 (4.9%) and 30 (37%) cases were be found positive for IgM and IgG against T. gondii in the ELISA method, respectively. Moreover, of 81 specimens, 42 (51.9%) and 39 (48.1%) samples had low-avidity IgG and high-avidity IgG by the IgG avidity kit, respectively. While, 59 (72.8%) of 81 samples were detected positive using the iPCR technique. Kappa (κ) value coefficient, between the iPCR and ELISA (for IgG) showed a strong agreement (0.360, p value < 0.001). A value of 0.25 I.U./ml for serum IgG [area under curve (AUC) = 0.720 (CI = 0.613-0.827); p = 0.002] was the cut-off value to differentiating between positive and negative toxoplasmosis (with sensitivity 66.0% and specificity 60.0%). Conclusion Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. gondii IgG antibody. However, more complementary investigations are widely needed in this regard.

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Prevalence of Toxoplasma gondii and Toxocara canis Among Myositis Patients in the Southwest of Iran

Saki

Mowla

Arjmand

et al. 2021

IDDT

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Introduction: Parasitic myositis is caused by some parasites such as T. gondii and T. canis. So, the aim of the study was to evaluate the prevalence T. gondii and T. canis in patients with myositis and healthy individuals. Methods: A total of 108 samples were randomly selected as the control (54 healthy individuals) and test (54 myositis patients) groups. IgG and IgM antibodies against T. gondii and IgG antibodies against T. canis were measured by the ELISA. The detection of chronic and acute toxoplasmosis was performed by the ELISA IgG avidity. The presence of T. gondii in blood was evaluated by the nested-PCR. Results: Of 108, 33 (30.6%) cases were detected positive for IgG against T. gondii that 19 (35.2%) and 14 (25.9%) were observed in myositis patients and healthy individuals, respectively (P=0.296). Of 19 positive cases, 12 (63.2%) and 7 (36.8%) cases were detected as chronic and acute toxoplasmosis, respectively, while, all positive cases in the control group had chronic toxoplasmosis (P=0.013). One (1.9%) sample was detected positive for anti- Toxoplasma gondii IgM and two (3.7%) samples were found positive for IgG against T. canis by the ELISA that these positive cases were observed only in myositis patients (P=1.000 P=0.495, respectively). B1 T. gondii gene was amplified in 12 (63.2%) and 1 (7.1%) in myositis patients and healthy subjects (P=0.001). Conclusions: Our findings showed that there was a relatively high prevalence of acute toxoplasmosis in myositis patients in comparison with the control subjects in southwest of Iran.

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Seroepidemiological study of toxoplasmosis in hemodialysis patients of Ahvaz, Southwest of Iran

Cited by 3 publications

References 10 publications

Impact of Toxoplasmosis in Im-mune Respons in Hemodialysis Patients

Impact of Toxoplasmosis in Im-mune Respons in Hemodialysis Patients

Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?

Prevalence of Toxoplasma gondii and Toxocara canis Among Myositis Patients in the Southwest of Iran

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