Seroepidemiology of Human Bocavirus in Hokkaido Prefecture, Japan

Endo, Rika; Ishiguro, Nobuhisa; Kikuta, Hídeaki; Teramoto, Shinobu; Shirkoohi, Reza; Ma, Xiaoming; Ebihara, Takashi; Ishiko, Hiroaki; Ariga, Tadashi

doi:10.1128/jcm.02140-06

Cited by 124 publications

(140 citation statements)

References 48 publications

Supporting

Mentioning

110

Contrasting

Unclassified

Order By: Relevance

“…However, a recent report (Guido et al, 2011) and the present work confirm that HBoV1 is also a frequent virus in adults with respiratory disease. On the other hand, since initially HBoV was detected in patients 5-6 months of age and older (Ma et al, 2006;Allander et al, 2007), and more than 90 % of infants younger than 3 months had specific antibodies, some authors proposed that maternal antibodies could prevent neonatal infection by HBoV (Endo et al, 2007). Yet here HBoV was detected among children 0-0.5 years old at a high frequency (24.4 %, Fig.…”

Section: Resultsmentioning

confidence: 68%

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

Ghietto

Cámara

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

Instituto de Virología 'Dr J. M. Vanella', Facultad de Ciencias Mé dicas, Universidad Nacional de Có rdoba, ArgentinaHuman bocavirus (HBoV) is a parvovirus with a possible aetiological role in respiratory disease that is currently under investigation. We detected HBoV1 in children and adults hospitalized with acute disease of the lower respiratory tract. HBoV genome was detected by PCR in nasopharyngeal swabs collected from 75 patients aged 0-89 years during 2010. HBoV was found in 17/75 (22.7 %) patients, 64.7 % of them infants younger than 1 year old and 29.4 % adults older than 30 years [the bimodal age distribution among HBoV-positive (HBoV + ) patients was statistically significant, P,0.001]. Of all HBoV + cases, 35.3 % were co-infected; all coinfections occurred in children (¡13 years old) and 83.3 % of them were HBoV-respiratory syncytial virus (RSV) co-infections. Among infants younger than 1 year, 50 % HBoV + specimens were co-infected, all of them with RSV. The rate of co-infection in infants was significantly higher compared to the frequency of co-infection in the whole cohort (P50.003). The results suggest that HBoV1 is involved in acute respiratory disease. Interplay between HBoV1 and RSV cannot be discarded as a cause of elevated percentages of co-detections in infants. INTRODUCTIONHuman bocavirus (HBoV) is a parvovirus first identified in 2005 in nasopharyngeal aspirates of children with lower respiratory tract infection (Allander et al., 2005). Since then, it has been associated with upper and lower acute respiratory infection (ARI), a leading cause of acute illnesses worldwide and the most important cause of mortality in infants and young children and disabilityadjusted life-years lost in developing countries (Simoes et al., 2006; WHO, 2009). To date, four species of HBoV have been proposed, and the name human bocavirus 1 (HBoV1) was suggested for the originally discovered virus. HBoV1 is linked to respiratory disease, while it is believed that HBoV2-4 are associated with gastroenteritis (Kapoor et al., 2010). With a ubiquitous distribution, the presence of HBoV DNA has been reported mostly in children with ARI in a variable range from 1.5 to 19 % (Allander, 2008), although recently even higher prevalence (33 %) has been observed in ill children (Martin et al., 2010). Although the virus is associated with ARI, the elevated rates of coinfection with other respiratory viruses with well-established pathogenic potential (Kaplan et al., 2006;Allander et al., 2007;Fry et al., 2007; Gerna et al., 2007;Kleines et al., 2007; Christensen et al., 2008 Christensen et al., , 2010Cilla et al., 2008;Pilger et al., 2011), the detection in asymptomatic individuals (Christensen et al., 2010) and the possibility of a persistent infection (Martin et al., 2010) make it difficult to allocate a causative role for HBoV in respiratory disease. Not only is the aetiological capacity of HBoV under investigation, but the natural history of the infection is still unknown. In addition, the impact of HBoV on the global epid...

show abstract

Section: Resultsmentioning

confidence: 68%

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

Ghietto

Cámara

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…This is similar to the single study so far reported relating to the seroprevalence of HBoV infection done in the Japanese population, using a immunofluorescence assay. The positive rate was lowest in the age group of 6 to 8 months and gradually increased with age: 42.3% in the 1 year group, 83.3% in the 2-3 years group, 89.5% in the 4-5 years group and 100% in 6-19 years group (Endo et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…At this time, neither virus isolation nor infectious clone has been reported. Serological study of HBoV infection in Japan has shown an overall seroprevalence rate of 71.1% against the VP1 protein of HBoV using an immunofluorescence assay in a population aged from 0 months to 41 years (Endo et al, 2007). Currently, detection of human bocavirus in children with lower respiratory tract infections relies on DNA amplification by PCR, however, PCR assays do not reflect the course of HBoV infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

View full text Add to dashboard Cite

Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation.

show abstract

“…In immunologic investigations by many other authors, it is apparent that up to 96% of healthy adults have past immunity to HBoV [19,21,25]. We speculate that the explanation for such high exposure could be that respiratory HBoV infections may provide efficient means by which the virus is perpetuated in the community throughout the years, and this is in turn serves to sustain a high level of seropositvity among populations in those communities with high seroprevalence.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates

Misigo

Mwaengo

Mburu

2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The commonly expected causative agents associated with flu-like symptoms in Kenya are the classical viral pathogens identifiable as influenza virus, adenovirus, parainfluenza virus, enteroviruses, respiratory syncytial virus (RSV) and rhinovirus. However, newer agents have been identified globally that present with illnesses clinically indistinguishable from those caused by the classical pathogens; one of them is human bocavirus. Methodology: A total of 384 specimens were analyzed, primarily to determine if the emerging human bocavirus (HBoV) infections exist in Kenya as coinfections with other respiratory viruses and to describe the genotype of the virus in circulation. In brief, viral nucleic acids were extracted from culture supernatants, amplified by PCR, and sequenced. Results: HBoV DNA was amplified from 1.8% of screened specimens. Coinfection with parainfluenza virus, adenovirus, and enterovirus was 2.5%, 2%, and 1.4%, respectively. Multiple coinfections consisting of HBoV plus two other viruses were found in 3% of specimens. Isolation occurred in the months of January, March, April, August, and November. Retrospective review of clinical parameters indicated that all the individuals complained of non-specific symptoms, mainly fever, coughs, nasal stuffiness, runny noses, and vomiting. Phylogenetically, the GenBank deposited sequences of this study's isolates cluster closely to the reference strain NC_07455 (HBoV1). Conclusion: Coinfections with human bocavirus (HBoV1) occur in Kenya, and high incidence might primarily be during the early stages of children's lives.

show abstract

Seroepidemiology of Human Bocavirus in Hokkaido Prefecture, Japan

Cited by 124 publications

References 48 publications

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Molecular detection and phylogenetic analysis of Kenyan human bocavirus isolates

Contact Info

Product

Resources

About