Seroepidemiology of Spotted Fever Rickettsiosis in Uttar Pradesh: A Prospective Study

Tripathi, Chandra Dev Pati; Singh, Mastan; Agarwal, Jyotsna; Kanta, Chandra; Atam, Virendra

doi:10.7860/jcdr/2017/25926.10029

Cited by 9 publications

(12 citation statements)

References 17 publications

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“…Aunque se puede utilizar esta prueba como elemento inicial de la valoración de los pacientes, se recomienda siempre pasar a otras pruebas como inmunofluorescencia, ELI-SA (por sus siglas en inglés Enzyme-linked Immunosorbent Assay) o PCR en los casos de alta sospecha clínica. A pesar de su alta especificidad, la prueba de PCR puede no detectar los casos cuando la prueba se hace en etapas tempranas de la enfermedad 18,19 . Este último punto nos permite considerar que, de los pacientes atendidos en el período de estudio, el número de casos pudo haber sido subestimado debido a que solo se incluyeron pacientes confirmados por PCR.…”

Section: Discussionunclassified

Fiebre manchada de montañas rocosas: experiencia en 5 años de vigilancia activa en un hospital pediátrico de segundo nivel en el noreste de México

López-Castillo

Aparicio

González-Soto

et al. 2019

BMHIM

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2012 a septiembre 2017 con diagnóstico confirmado clínicamente y por reacción en cadena de la polimerasa (PCR) de FMMR. Se presentan los antecedentes epidemiológicos, las características clínicas y evolución de los pacientes. Resultados: Se confirmaron 14 pacientes. La relación masculino:femenino fue de 1.8:1, la edad promedio de los pacientes fue de 7.6 años (18 meses a 13 años). El 42.8% de los pacientes refirió el contacto con perros y el 57.1% afirmó tener contacto con garrapatas. En todos los casos hubo fiebre y exantema purpúrico; alrededor del 70% manifestaron mialgias y artralgias; el 28% tuvo sangrado del tubo digestivo, y el 11% alteraciones neurológicas graves. El 64.2% de los casos recibió tratamiento adecuado con doxiciclina. Fallecieron 8 pacientes, con una tasa de letalidad de 57.1%. Conclusiones: La zona sur de Coahuila debe considerarse una zona endémica para FMMR. El retraso en el diagnóstico y tratamiento favorecen una mayor letalidad.

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Section: Discussionunclassified

Fiebre manchada de montañas rocosas: experiencia en 5 años de vigilancia activa en un hospital pediátrico de segundo nivel en el noreste de México

López-Castillo

Aparicio

González-Soto

et al. 2019

BMHIM

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“…Eschar was reported in only 3% of the positive cases. [27] In our study, we found 4 (0.36%) samples positive for Rickettsia among 1101 samples by genus-specific real-time PCR that amplifies all rickettsial species. The IgM ELISA each specific to the SFG and TG was individually tested with randomly generated 178 samples.…”

Section: Discussionmentioning

confidence: 50%

Standardization and Evaluation of an In-house Multiplex Real-time Polymerase Chain Reaction for Simultaneous Detection of Pathogenic Species of Brucella, Rickettsia, and Leptospira in Patients with Acute Febrile Illness

Saravanan

Rajendiran

Sankar

et al. 2022

APJHS

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Background and Objectives: Acute febrile illness is caused by a wide range of etiologies. Brucella, Rickettsia and Leptospira as a cause of acute febrile illness have not been well studied. Identification by culture is laborious and diagnosis is often based on serological tests. We aimed to develop an in-house multiplex real-time PCR assay for the simultaneous detection of Brucella, Rickettsia and Leptospira and evaluate on samples collected from patients presenting with acute febrile illness. Methods: Samples (n=1101) were collected from patients presenting with acute febrile illness from different regions. An inhouse multiplex real-time PCR was developed for the specific detection of Brucella species, Rickettsia species and pathogenic species of Leptospira. The assay was evaluated on clinical samples. IgM ELISA was carried out on randomly selected samples (n=178). Results: The detection limit of the multiplex real-time PCR assay was 6.3, 43.7 and 1.2 genome copies per 10 μl of PCR input for Brucella, Rickettsia and Leptospira respectively. Among 1101 samples, Leptospira was positive in 1.36% samples and Rickettsia was positive in 0.36% samples. Among random samples, 37.1% of samples were positive for Brucella IgM, 19.6% were positive for Rickettsia IgM and 11.2% were positive for Leptospira IgM. Interpretation and Conclusions: The study showed a high seroprevalence of Brucellosis and Rickettsiosis among the samples. The in-house multiplex real-time PCR assay will be a useful tool in the syndromic diagnosis for a specific and comprehensive laboratory diagnostic testing.

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“…In a similar study, Indian researchers tested 432 blood samples collected from patients with fever of unknown origin that might resemble undifferentiated febrile illness and detected 115 (26.6%) samples positive for SFG rickettsiosis. 40 Most positive samples were found in the group aged 0-15 years. Most of their patients were from rural areas and were farmers and self-employed, as in our study.…”

Section: Discussionmentioning

confidence: 94%

“…It is also suitable for seroepidemiological studies. 36,[38][39][40] This serological investigation for both rickettsial groups had a number of challenges. Paired serum sample and complete questionnaires were available from only 84.4% (N = 802) of febrile patients (N = 950).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Anti-Rickettsia spp. Antibodies in Hospitalized Patients with Undifferentiated Febrile Illness in the Southern Region of Kazakhstan

Turebekov¹,

Abdiyeva²,

Yegemberdiyeva

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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Abstract.Undifferentiated febrile illness still represents a demanding medical problem all over the world, but primarily in low- and middle-income countries. Scientific and clinical investigations related to undifferentiated febrile illness and rickettsial diseases in Kazakhstan are lacking. This study reflects the investigation of antibodies against spotted fever group (SFG) and typhus group (TG) rickettsiae in patients with undifferentiated febrile illness in the southern region of Kazakhstan (Almaty and Kyzylorda oblasts). Paired serum samples were gathered from 13 hospitals in these two oblasts and explored for the presence of IgM and IgG antibodies against typhus group and IgG antibodies against spotted fever group rickettsiae using ELISA. Patient’s questionnaires were statistically analyzed. In total, 802 inpatients from Almaty (N = 9) and Kyzylorda (N = 4) hospitals were included in this research. Based on ELISA results, 250 patients out of 802 (31.2%) from both oblasts had IgG antibodies against SFG rickettsiae. Results from 11 (1.4%) patients indicated acute infection with tick-borne rickettsiosis. Regarding TG rickettsiae (R. typhi), a past infection was detected in 248 (30.9%) febrile patients and acute infection in 22 (2.7%) patients in the two selected oblasts. The data indicated that SFG and TG rickettsioses are present in Kazakhstan. Kazakh physicians should be aware of these emerging diseases in both investigated oblasts because the occurrence of these diseases is not suspected during day-to-day clinical practice. The identification of rickettsial pathogens and implementation of modern laboratory methods for the diagnostics of rickettsioses are in need throughout Kazakhstan.

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Seroepidemiology of Spotted Fever Rickettsiosis in Uttar Pradesh: A Prospective Study

Cited by 9 publications

References 17 publications

Fiebre manchada de montañas rocosas: experiencia en 5 años de vigilancia activa en un hospital pediátrico de segundo nivel en el noreste de México

Fiebre manchada de montañas rocosas: experiencia en 5 años de vigilancia activa en un hospital pediátrico de segundo nivel en el noreste de México

Standardization and Evaluation of an In-house Multiplex Real-time Polymerase Chain Reaction for Simultaneous Detection of Pathogenic Species of Brucella, Rickettsia, and Leptospira in Patients with Acute Febrile Illness

Occurrence of Anti-Rickettsia spp. Antibodies in Hospitalized Patients with Undifferentiated Febrile Illness in the Southern Region of Kazakhstan

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