Serogroups and genotypes of<i>Leptospira</i>spp. strains from bovine aborted foetuses

Delooz, Laurent; Czaplicki, Guy; Grégoire, Fabien; Pozzo, Fabiana Dal; Pez, Floriane; Kodjo, Angéli; Saegerman, Claude

doi:10.1111/tbed.12643

Cited by 33 publications

(44 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vibrio metschnikovii has been identified by bacteriological culture in cattle, swine and horse abortions [ 48 ], and L. lactis , which is mainly associated with bovine mastitis [ 49 ], has also been isolated from a bovine abortion [ 43 ]. It is likely that the suspected abortive agents, unidentified Pseudomonas , S. uberis and E. coli , may spread via the amniotic-oral route to the fetus and produce infection (Table 3 ); however, causality between these pathogens and abortions has not been confirmed [ 42 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Vidal

Kegler

Posthaus

et al. 2017

Vet Res

View full text Add to dashboard Cite

Abortions in cattle have a significant economic impact on animal husbandry and require prompt diagnosis for surveillance of epizootic infectious agents. Since most abortions are not epizootic but sporadic with often undetected etiologies, this study examined the bacterial community present in the placenta (PL, n = 32) and fetal abomasal content (AC, n = 49) in 64 cases of bovine abortion by next generation sequencing (NGS) of the 16S rRNA gene. The PL and AC from three fetuses of dams that died from non-infectious reasons were included as controls. All samples were analyzed by bacterial culture, and 17 were examined by histopathology. We observed 922 OTUs overall and 267 taxa at the genus level. No detectable bacterial DNA was present in the control samples. The microbial profiles of the PL and AC differed significantly, both in their composition (PERMANOVA), species richness and Chao-1 (Mann–Whitney test). In both organs, Pseudomonas was the most abundant genus. The combination of NGS and culture identified opportunistic pathogens of interest in placentas with lesions, such as Vibrio metschnikovii, Streptococcus uberis, Lactococcus lactis and Escherichia coli. In placentas with lesions where culturing was unsuccessful, Pseudomonas and unidentified Aeromonadaceae were identified by NGS displaying high number of reads. Three cases with multiple possible etiologies and placentas presenting lesions were detected by NGS. Amplicon sequencing has the potential to uncover unknown etiological agents. These new insights on cattle abortion extend our focus to previously understudied opportunistic abortive bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Vidal

Kegler

Posthaus

et al. 2017

Vet Res

View full text Add to dashboard Cite

show abstract

“…Domestic animals such as bovines (Collares-Pereira, 1991) and pigs (Rocha, 1990) can also become chronic renal carriers after infection (Bomfim, Barbosa-Stancioli, & Koury, 2008;Fearnley et al, 2008). In livestock, leptospirosis is particularly important and results in abortion, stillbirths, infertility, poor milk production and death, inducing important economic losses (Delooz et al, 2018;Ellis, 2015). Both wild and domestic animals can be a source of infection for humans, which are considered incidental hosts.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of pathogenic leptospires from wild, domestic and captive host species in Portugal

Ferreira

Ahmed

Rocha

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Leptospirosis is a neglected zoonotic disease of worldwide distribution with a significant veterinary and public health impact. It is caused by pathogenic bacteria of the genus Leptospira. The availability of effective tools to accurately identify and type leptospires is of utmost importance for the diagnosis of the disease and for assessing its epidemiology. Several multi‐locus sequence typing (MLST) approaches were described for the typing of worldwide isolates of Leptospira but an extensive agreement towards the adoption of a unique consensus scheme for this agent is still lacking. Most genotyped strains originate from Asian and South American countries, with a minority originating from Europe (being most countries represented only by one or a few isolates). The knowledge of the diversity of circulating leptospires is the key to understanding the disease transmission and its zoonotic implications. In this study, we revisited the taxonomy of several isolates of pathogenic Leptospira obtained from domestic, wild and captive animals in Portugal, between 1990 and 2012. A selection of these isolates was genotyped using two previously published MLST schemes. A total of seven distinct sequence types (STs) were detected among the Portuguese isolates with two STs representing L. borgpetersenii (ST149 and ST152), two STs representing L. kirschneri (ST117 and ST100) and three STs representing L. interrogans (ST17, ST24 and ST140). Global widespread (and maybe more virulent) Leptospira genotypes seem to circulate in Portugal, particularly the L. interrogans ST17 isolates which are associated with several outbreaks of leptospirosis among humans and animals in different regions of the world. This study contributes to the enrichment of the global MLST databases with a new set of allele and sequence type information also providing novel data on circulating Leptospira serovars in Portugal.

show abstract

“…In addition to human hosts, there is a variety of animal hosts for pathogenic leptospires with different serovars. So far, at least 200 species of domestic mammals and wild animals covering Pisces, Amphibia, Reptilia, Aves and Mammalia have been confirmed to serve as hosts, although the epidemiology of leptospirosis and the role of animals is complex and highly dependent on the specific serovars (Ballados‐González et al, ; Delooz et al, ; Hamond et al, ; Zhang, Wang, & Yan, ). Rodents and domestic animals (pigs, dogs, cattle, horses and sheep) are considered the main reservoirs for Leptospira infections in humans (Ellis, ; Guernier et al, ; Zhang, Xu, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Leptospirosis trends in China, 2007–2018: A retrospective observational study

Zhang

Zhu

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Leptospirosis is one of the most common and neglected tropical waterborne diseases in China, causing serious economic losses, and constituting a significant public health threat. Leptospirosis has recently received increased attention and is considered a reemerging infectious disease in many countries. The incidence of leptospirosis among people suggests that occupation, age, season, sex and water recreational activities are significant risk factors. The aim of this study was to describe the epidemiological profiles of leptospirosis in China during the 2007-2018 period. The morbidity data of leptospirosis by age, season (month), gender, occupation and geographic location (different provinces) were obtained from the public health science data centre of China for subsequent epidemiological analysis. The results indicate that the incidence of leptospirosis has shown a slow downward trend from 2007 to 2018, but morbidity rates were still relatively high (0.0660-0.0113). The incidence of leptospirosis varied in different provinces of China; cases localized mainly to the Southern and Central provinces, areas with warm weather and ample rainfall. Older people (aged 60-75), males, farmers, students and field workers were high-risk populations. During the 2007-2018 observation period, morbidity rates increased beginning in May, remained at high levels in August and September and decreased after November. The present investigation highlights the re-emergence of leptospirosis in some provinces of China (especially in Yunnan and Fujian) and shows that leptospirosis remains a serious public health threat. The results of this study should enhance measures taken for the prevention, control, and surveillance of leptospirosis in China.

show abstract

Serogroups and genotypes ofLeptospiraspp. strains from bovine aborted foetuses

Cited by 33 publications

References 22 publications

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Genetic diversity of pathogenic leptospires from wild, domestic and captive host species in Portugal

Leptospirosis trends in China, 2007–2018: A retrospective observational study

Contact Info

Product

Resources

About