Serologic characteristics of a human rotavirus isolate, AU‐1, which has a “long” RNA pattern and subgroup I specificity

Kitaoka, Setsuko; Nakagomi, Toyoko; Fukuhara, Norio; Hoshino, Yasutaka; Sano, Hiroshi; Nakagomi, Osamu; Kapikian, Albert Z.; Ebina, Takusaburo; Konno, Tasuke; Ishida, Nakao

doi:10.1002/jmv.1890230407

Cited by 35 publications

(17 citation statements)

References 25 publications

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“…The sequence data identified two different P-G combinations, P[9],G6 and P[14],G6, among Hungarian G6 rotaviruses. These combinations are identical with those of other human G6 isolates described previously Palombo and Bishop, 1995;Arista et al, 1999;Griffin et al, 2002] [Kitaoka et al, 1987;Taniguchi et al, 1989;Gerna et al, 1990;Urasawa et al, 1993]). A previous study suggested that the VP7 genes of human G6 strains with different P serotypes formed distinct genetic clusters [Palombo and Bishop, 1995].…”

Section: Discussionsupporting

confidence: 77%

Genetic variability among serotype G6 human rotaviruses: Identification of a novel lineage isolated in Hungary

Bányai

Gentsch

Griffin

et al. 2003

Journal of Medical Virology

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Rotavirus serotype G6 has been demonstrated to be a rare cause of gastroenteritis in man. To date, only a few well characterized strains have been described from Italy, Australia, and the United States. Nucleotide sequencing of G6 VP7 genes shows that these strains belong to two distinct G6 lineages, one for strains of serotype P11[14],G6 (PA169-like strains) and one for strains of serotype P3[9],G6 (PA151-like strains). In this study, we sequenced the VP7 genes and VP8* gene fragments of human rotavirus G6 strains detected in Hungary. Phylogenetic analysis demonstrated that the VP7 genes of Hungarian G6 strains fell into three lineages, represented by a single PA169-like strain, three PA151-like strains, and two novel G6 strains, respectively. The amino acid sequence identity of VP7 was 97.2-100% within each lineage and 92-93.9% between any two lineages. The sequence analysis of VP8* revealed that the single PA169-like Hungarian G6 strain belonged to genotype P[14] and was phylogenetically closely related to P11[14],G6 strains characterized previously. In contrast, the VP8* of PA151-like Hungarian G6 strains clustered in accordance with their VP7 genes representing genetically distinguishable variants of genotype P[9]. This finding raises the possibility that Hungarian genotype P[9],G6 strains might have been generated through independent reassortment events. Serotype G6-specific primers for each human G6 lineage were also developed. The use of these primers in reverse-transcription polymerase chain reaction genotyping may help determine the epidemiological role of G6 strains in humans.

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Section: Discussionsupporting

confidence: 77%

Genetic variability among serotype G6 human rotaviruses: Identification of a novel lineage isolated in Hungary

Bányai

Gentsch

Griffin

et al. 2003

Journal of Medical Virology

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“…In addition, these three HRV strains were unusual, in that they possessed a subgroup I (instead of subgroup II) specificity and appeared to be more closely related to animal rather than human strains in RNA-RNA hybridization studies (Gerna et al, manuscript submitted). Serotype 3 HRV strains with subgroup I specificity, one of which genetically related to feline RV, have already been reported from Japan [24,28,30]. In addition, unusual combinations of serotype spec-ificity, subgroup specificity and RNA electropherotype have been reported in routine examination of fecal HRV strains [1,2,19,33].…”

Section: Discussionmentioning

confidence: 93%

Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype

Gerna

Sarasini

Zentilin

et al. 1990

Archives of Virology

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During an epidemiological study on the prevalence of human rotavirus (HRV) serotypes 1-4 in Europe, we found that some strains could not be typed. However, when a monoclonal antibody directed to serotype 8 HRV was included in the typing assay, we detected seven 69 M-like (serotype 8) strains, six from Finland and one from Italy. The previously reported serotype 8 HRV strains, 69 M, B 37, and B 38 isolated in Indonesia, were of subgroup I specificity and presented a peculiar "super short" RNA electropherotype. In contrast, all the seven European strains possessed a long RNA pattern, and one of them had subgroup II specificity. Three of these strains were adapted to growth in cell cultures and were further characterized by neutralization and by Northern blot hybridization. They appeared to be closely related to serotype 8 HRV strain 69 M by neutralization, but showed partial homology with several human and animal strains by hybridization. The epidemiological importance of these serotype 8 strains circulating in Europe should be investigated, in view of their possible inclusion in a rotavirus vaccine.

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“…However, the antigenicity of the novel strains detected in this study was quite different from that ofAU-1 strain. The AU-1 strain was neutralized specifically by an antiserum to serotype 3 [18] and the VP7 gene of the AU-1 strain had a high homology with those of serotype 3 strains [22]. In contrast, based on the results of the neutralization tests using the four isolated strains and serotype specific polyclonal and MAbs, our subgroup I strains seem to have a new serotype specificity other than the four established serotype specificities.…”

Section: Discussionmentioning

confidence: 93%

Unusual human rotavirus strains having subgroup I specificity and ?long? RNA electropherotype

Kobayashi

Lintag

Urasawa

et al. 1989

Archives of Virology

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During an epidemiological study of human rotavirus in Metro Manila, Philippines, we found 20 unusual strains which belong to subgroup I but have "long" RNA pattern typical of subgroup II human rotavirus. The RNA patterns of the 20 strains were classified into four groups though they were very similar to each other. Four strains, designated L4, L26, L27, and L34 were isolated in MA104 cells from stool specimens. They possessed subgroup I specificity and long RNA pattern identical to that of the viruses in the original stool samples. The serotype specificity of these strains could not be determined by either enzyme-linked immunosorbent assay (ELISA) or neutralization test, while one strain (L27) was neutralized by serotype 2-specific anti-VP4 monoclonal antibody. These strains were suggested to have an unusual antigenicity on VP7.

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Serologic characteristics of a human rotavirus isolate, AU‐1, which has a “long” RNA pattern and subgroup I specificity

Cited by 35 publications

References 25 publications

Genetic variability among serotype G6 human rotaviruses: Identification of a novel lineage isolated in Hungary

Genetic variability among serotype G6 human rotaviruses: Identification of a novel lineage isolated in Hungary

Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype

Unusual human rotavirus strains having subgroup I specificity and ?long? RNA electropherotype

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