Serologic evidence of Ureaplasma urealyticum infection in women with spontaneous pregnancy loss

Quinn, Patrick G.; Shewchuk, Alan B.; Shuber, J.; Lie, K.I.; Ryan, E.; Sheu, Ming-Huei; Chipman, Mary L.

doi:10.1016/0002-9378(83)90500-8

Cited by 64 publications

(38 citation statements)

References 6 publications

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“…U. parvum is most commonly isolated by culture (1,25). However, U. urealyticum is isolated significantly more often from women with infertility, miscarriages, and pelvic inflammatory disease (1,28,29). In the present study, U. parvum was also isolated most often by culture (83%, Table 3).…”

Section: Discussionmentioning

confidence: 42%

Comparison of Multiplex PCR Assay with Culture for Detection of Genital Mycoplasmas

et al. 2004

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Ureaplasma, spp. Mycoplasma genitalium, and Mycoplasma hominis are associated with infection of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. We have developed a multiplex PCR for the detection of these Mycoplasmatales in a single amplification reaction. The analytical sensitivities of this assay were 10.8, 10.8, and 8.8 CFU for each organism, respectively. This multiplex PCR was compared to culture on 26 cervical swabs, 2 vaginal swabs, 4 female urine specimens, 49 semen samples, 2 male urine specimens, and 1 nonspecified sample. A total of 21 specimens were culture positive (25%); 17 of these were PCR positive. An additional 11 specimens were PCR positive but culture negative. Of the 21 culture-positive specimens, 17 (81%) grew Ureaplasma spp. and 4 (19%) grew Mycoplasma spp. Of the 28 PCR-positive specimens, Ureaplasma spp. was detected in 23 (82%), M. hominis was detected in 3 (11%), and both were detected in 2 (7%). In a confirmatory analysis, all samples were tested by amplification of a second target of the ureaplasma genome. True-positive cases were defined as a positive result by culture or by both amplification assays. The multiplex PCR detected organisms in 26 of the 30 true-positive specimens, as well as in 2 other specimens. Based on a 36% prevalence of infection, the sensitivity, specificity, and positive and negative predictive values of multiplex PCR analyses were 87, 96, 94, and 93%, respectively. Multiplex PCR offers a rapid, sensitive, and easy method to detect genital mycoplasmas.

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Section: Discussionmentioning

confidence: 42%

Comparison of Multiplex PCR Assay with Culture for Detection of Genital Mycoplasmas

et al. 2004

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“…Data reported by Quinn et al (225)(226)(227) also suggest that selective antibody to certain serotypes increases in women with pregnancy wastage and in infants with respiratory disease compared with control patients. Work by Gallo et al (90) suggests that the presence of U. urealyticum-specific IgM antibody in infants is predictive of disease.…”

Section: Host Defenses In the Neonatementioning

confidence: 90%

“…Prior to division of the two biovars into separate species, numerous studies utilizing a direct approach to typing the organisms grown from culture by a variety of techniques such as polyclonal or monoclonal antibodies (81), immunofluorescence (198), immunoperoxidase (222), agar growth inhibition (259), and an indirect approach using serology to measure antibody responses to specific serotypes in targeted patient populations (226,227) attempted to ascertain differential pathogenicity of ureaplasmas at the serotype level. Results were varied and inconsistent due to a great extent to the inefficient and imprecise methods available for serotype differentiation at the time, occurrence of multiple cross-reactions, and the fact that many persons may harbor more than one serotype in their urogenital tract in the presence or absence of disease.…”

Section: Other Infectionsmentioning

confidence: 99%

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

Katz²,

2005

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SUMMARY The genital mycoplasmas represent a complex and unique group of microorganisms that have been associated with a wide array of infectious diseases in adults and infants. The lack of conclusive knowledge regarding the pathogenic potential of Mycoplasma and Ureaplasma spp. in many conditions is due to a general unfamiliarity of physicians and microbiology laboratories with their fastidious growth requirements, leading to difficulty in their detection; their high prevalence in healthy persons; the poor design of research studies attempting to base association with disease on the mere presence of the organisms in the lower urogenital tract; the failure to consider multifactorial aspects of diseases; and considering these genital mycoplasmas only as a last resort. The situation is now changing because of a greater appreciation of the genital mycoplasmas as perinatal pathogens and improvements in laboratory detection, particularly with regard to the development of powerful molecular nucleic acid amplification tests. This review summarizes the epidemiology of genital mycoplasmas as causes of neonatal infections and premature birth; evidence linking ureaplasmas with bronchopulmonary dysplasia; recent changes in the taxonomy of the genus Ureaplasma; the neonatal host response to mycoplasma and ureaplasma infections; advances in laboratory detection, including molecular methods; and therapeutic considerations for treatment of systemic diseases.

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“…Antibody titers to U. urealyticum are higher in mothers with a history of fetal wastage (99). However, the results of all of these epidemiologic studies are difficult to interpret since the comparability of the various groups of women is uncertain and the role of other microorganisms was often not assessed.…”

Section: Morbidity and Mortalitymentioning

confidence: 99%

“…In general, patient and control groups have been poorly characterized and the numbers in the groups have been small. In some instances, detection of serovar-specific antibody has been used in the absence of cultural isolation and documentation of the serovars present (97,99). Only one group of investigators has used antisera to all 14 serovars, and only one group has performed typing directly on the primary isolation plate (104,126).…”

Section: Neonatal Sepsismentioning

confidence: 99%

Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns

et al. 1993

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Ureaplasma urealyticum, a common commensal of the urogenital tract of sexually mature humans, is gaining recognition as an important opportunistic pathogen during pregnancy. While its etiologic significance in many aspects of adverse pregnancy remains controversial, recent evidence indicates that U. urealyticum in the absence of other organisms is a cause of chorioamnionitis. Furthermore, ureaplasmal infection of the chorioamnion is significantly associated with premature spontaneous labor and delivery. In at least some cases, it appears to be causal. Present evidence indicates that U. urealyticum is a cause of septicemia, meningitis, and pneumonia in newborn infants, particularly those born prematurely. There is strong but not definitive evidence that ureaplasmal infection of the lower respiratory tract can lead to development of chronic lung disease in very low-birth-weight infants. Although risk factors for colonization of the lower genitourinary tract have been identified, little information is available concerning risk factors for intrauterine infection and host immune responses to invasive infection. Recent establishment of animal models of respiratory and central nervous system diseases should provide an opportunity to evaluate risk factors, pathogenic mechanisms, and operative immune mechanisms. However, the most critical need is additional information concerning indications for diagnosis and treatment as well as efficacy of treatment.

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Serologic evidence of Ureaplasma urealyticum infection in women with spontaneous pregnancy loss

Cited by 64 publications

References 6 publications

Comparison of Multiplex PCR Assay with Culture for Detection of Genital Mycoplasmas

Comparison of Multiplex PCR Assay with Culture for Detection of Genital Mycoplasmas

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns

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