Serologic Testing in Connective Tissue Diseases

Habash-Bseiso, Dana E.; Yale, Steven H.; Glurich, Ingrid; Goldberg, Jerry W.

doi:10.3121/cmr.3.3.190

Cited by 44 publications

(26 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is in accordance with other previously conducted studies (Tsokos, 2011;Isenberg et al, 2007). Detection of this type of antinuclear antibodies is highly suggestive of SLE disease, but a negative result of these antibodies in patient's specimen does not exclude the presence of such a disease (Habash-Bseiso et al, 2005). Possible occurrence of anti-dsDNA antibody in asymptomatic lupus patients should pay attention that this test should not be relied upon as a definite parameter to follow up disease activity (Kavanaugh and Solomon, 2002 Table 4 shows sensitivity and specificity of antidsDNA test in lupus nephritis patients using ELISA test.…”

Section: Resultssupporting

confidence: 81%

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Manhal¹,

Mohammed²

2016

American Journal of Immunology

View full text Add to dashboard Cite

Abstract:The majority of patients with Lupus Nephritis (LN) usually have abnormal findings of kidney function tests. Severe glomerular damage may be observed in some patients and requires prompt therapeutic interventions. High titer levels of anti-ds-DNA antibodies may correlate to some extent with disease activity in lupus nephritis patients. The purpose of this study was to evaluate the relationship between anti-ds-DNA antibody titers and abnormal kidney function tests in patients with lupus nephritis. A total of seventy patients with lupus nephritis and fifty healthy controls were enrolled in this study. Blood samples were collected and labeled from study patients and controls for certain hematological, biochemical and immunological investigations. Anti-ds-DNA antibodies were tested by using IgG-ELISA test. It was shown that 74% of lupus nephritis patients showed positive results for anti-ds-DNA antibodies in their serum specimens (p-value <0.01). Sensitivity and specificity of the anti-ds-DNA antibody test for the diagnosis of lupus nephritis were 74 and 100%, respectively. Anaemia, hypoalbuminemia, fasting hyperglycemia and elevated blood urea nitrogen were significantly associated with lupus nephritis activity. Further studies are required to study genomic and unprecedented biomarkers associated with anti-ds-DNA antibodies in patients with lupus nephritis to develop our perception of this autoimmune disease.

show abstract

Section: Resultssupporting

confidence: 81%

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Manhal¹,

Mohammed²

2016

American Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In keeping with the female bias in rheumatic diseases, females predominated (n=117, 74.5%) (p<0.0001). Positive ANA was seen in 95 ANA-CTD cases (60.5%) (table 1), and consistent with the literature,1 2 positivity was seen mainly in scleroderma/systemic sclerosis (88.1%, n=37) and SLE (75.7%, n=28).…”

supporting

confidence: 89%

“…The antinuclear antibody (ANA) test is one of the most common autoimmunity blood tests, which plays a major role in the diagnosis of several connective tissue diseases (CTDs), including scleroderma/systemic sclerosis and systemic lupus erythematosus (SLE) 1. We performed a brief investigation to examine the performance of ANA in ANA-associated CTDs (ANA-CTD) at one Australian teaching tertiary hospital.…”

mentioning

confidence: 99%

Evaluation of antinuclear antibody (ANA) in ANA-associated connective tissue diseases

2015

View full text Add to dashboard Cite

“…However, these results are at least competitive with antinuclear antibody (ANA) testing for systemic lupus, which is highly specific (97%) but has low sensitivity (57%) (c.f. Table 2 in [50]. A further note is that the classification performance of the thermograms was achieved via comparison with a heterogeneous mixture of control subjects with a variety of co-morbidities including rheumatoid arthritis, osteoarthritis, anemia, high blood pressure, and diabetes, among others.…”

Section: Discussionmentioning

confidence: 99%

Differential scanning calorimetry as a complementary diagnostic tool for the evaluation of biological samples

Garbett

Brock

2016

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

Background Differential scanning calorimetry (DSC) is a tool for measuring the thermal stability profiles of complex molecular interactions in biological fluids. DSC profiles (thermograms) of biofluids provide specific signatures which are being utilized as a new diagnostic approach for characterizing disease but the development of these approaches is still in its infancy. Methods This article evaluates several approaches for the analysis of thermograms which could increase the utility of DSC for clinical application. Thermograms were analyzed using localized thermogram features and principal components (PCs). The performance of these methods was evaluated alongside six models for the classification of a data set comprised of 300 systemic lupus erythematosus (SLE) patients and 300 control subjects obtained from the Lupus Family Registry and Repository (LFRR). Results Classification performance was substantially higher using the penalized algorithms relative to localized features / PCs alone. The models were grouped into two sets, the first having smoother solution vectors but lower classification accuracies than the second with seemingly noisier solution vectors. Conclusions Coupling thermogram technology with modern classification algorithms provides a powerful diagnostic approach for analysis of biological samples. The solution vectors from the models may reflect important information from the thermogram profiles for discriminating between clinical groups. General significance DSC thermograms show sensitivity to changes in the bulk plasma proteome that correlate with clinical status. To move this technology towards clinical application the development of new approaches is needed to extract discriminatory parameters from DSC profiles for the comparison and diagnostic classification of patients.

show abstract

Serologic Testing in Connective Tissue Diseases

Cited by 44 publications

References 6 publications

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Evaluation of antinuclear antibody (ANA) in ANA-associated connective tissue diseases

Differential scanning calorimetry as a complementary diagnostic tool for the evaluation of biological samples

Contact Info

Product

Resources

About