Serologic Tests of IgG and IgM Antibodies and IgG Avidity for Diagnosis of Ocular Toxoplasmosis

Esboei, Bahman Rahimi; Zarei, Mohammad; Mohebali, Mehdi; Valian, Hossein Keshavarz; Shojaee, Saeedeh; Mahmoudzadeh, Raziyeh; Salabati, Mirataollah

doi:10.3347/kjp.2018.56.2.147

Cited by 26 publications

(20 citation statements)

References 28 publications

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“…With the IgG avidity method, the results showed that 98.35% of the samples had high avidity, indicating that ocular toxoplasmosis caused by reactivation of chronic infection of toxoplasma. This is similar to the results by Rahimi-Esboei et al who reported that 87.2% of the cases had high IgG avidity in Iran-Teheran in their prospective study on patients with ocular toxoplasmosis (OT) who referred to the Farabi Eye Hospital, Tehran, Iran [32]. Also our result consistent with the results by Jones et al [33] who showed that 11.7% of their cases in the USA have recently acquired infection and 88.3% were IgG positive/IgM negative, which indicated they had chronic ocular toxoplasmosis.…”

Section: Discussionsupporting

confidence: 90%

Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

2020

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Introduction Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debilitating vision loss.In Khuzestan province, southwest of Iran, T. gondii infection has been reported to be significant but toxocariasis was rare. However, the frequency of ocular toxoplasmosis and toxocariasis has not been studied in this area. The aim of this study was to evaluate the ocular toxoplasmosis and ocular toxocariasis using serological and molecular methods. Method In this case control study, 310 patients were identified by ophthalmologist as ocular toxoplasmosis and then 5 cc of venous blood samples were taken from each of them. Serum samples and buffy coat were prepared and ELISA was used to detect IgG and IgM anti-Toxoplasma antibodies and the molecular PCR was used to detect Toxoplasma DNA parasite in buffy coats. ELISA test was used to detect of IgG anti-Toxocara antibodies. Results Totally, for ocular toxoplasmosis, 130 (41.93%) of 310 patients were positive by ELISA, of them 121 (39%) IgG positive and nine (2.9%) IgM positive were diagnosed. Of 121 cases with IgG+, 119 (98.35%) were diagnosed with high IgG avidity indicating chronic phase of the infection. For ocular toxocariasis evaluation, antibodies against Toxocara were not detected in any of the samples. By PCR molecular method, 11 out of 310 patients (3.54%) had T. gondii DNA in the blood. In control, in total, 21 cases were detected positive by serology method, which showed a significant difference with the results of the case group(P < 0.05).By PCR method, only three cases showed positive which also indicated significant difference with result of case group (3 vs 9) (P < 0.05). In the control group, also no anti-toxocara antibodies were found. Conclusion It can be concluded that T. gondii in Khuzestan province as the etiologic agent of ocular toxoplasmosis and physicians should consider diagnostic methods for identifying the infection when they visit the patients.

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Section: Discussionsupporting

confidence: 90%

Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

2020

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“…During October 2018 to July 2019, fifty-six samples including corneal scrapes, contact lenses or its maintenance solution were collected from keratitis patients referred to the Farabi eye teaching hospital, Tehran city, capital of Iran ( Esboei et al, 2019 ; Rahimi-Esboei et al, 2018 ). All clinical samples were inspected by both direct microscopic and culture methods.…”

Section: Methodsmentioning

confidence: 99%

Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran

Esboei

Fakhar

Saberi

et al. 2020

Parasite Epidemiology and Control

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Objective Acanthamoeba keratitis cause severe corneal infection and lead to poor vision and blindness. This disease is caused by a unicellular amphizoic protozoon called Acanthamoeba spp. that present in different environments. This study aimed to represent the existence and genotyping of Acanthamoeba spp. in patients with keratitis and swimming pool water (SPW) in Tehran Province, Central Iran. Methods In this descriptive study, 56 clinical samples were collected from patients with keratitis and 30 water samples were collected from different swimming pools in Tehran Province. All samples were examined based on the morphological and molecular techniques. The genotypes were determined by sequencing the partial of 18S rRNA gene. Results Of 56 clinical (corneal) and 30 environmental (SPW) samples, 30.3% and 40.0% were positive for Acanthamoeba spp., respectively. According to sequencing analysis, 94.1% of amoebic keratitis isolates were belonged to T4 genotype and only one (5.8%) isolate was belonged to T11 genotype. All genotypes were detected from SPW samples were identified as T4 genotype. Conclusion According to our results, use of contact lens and swimming in pool poses the major risk factor for amoebic keratitis in the studied area (Tehran). Moreover, T4 genotype was the predominant genotype of human keratitis and swimming pool samples there. Consequently, essential and practical measures are urgently needed to prevent subjects against this ocular seriously disease.

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“…Also, molecular methods such as polymerase chain reaction may be useful for the detection of the infection by amplification of conserved sequences of parasite genes (Marín et al 2018 ). Despite the availability of these laboratory analyses for toxoplasmosis diagnostic, the confirmation of ocular toxoplasmosis is usually clinical and when the clinical examination is not sufficient to confirm its presence, the laboratory diagnostic involves the analyze of ocular fluids (Greigert et al 2019a ; Rahimi-Esboei et al 2018 ; Rahimi Esboei et al 2019 ) using polymerase chain reaction and immunoblot techniques (Greigert et al 2019b ). Thus, there is an interest in finding new markers and predictors of disease useful for ocular toxoplasmosis diagnostics and monitoring, and some routine laboratory tests may be better explored for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis

et al. 2020

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Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, which can be acquired by oral contact and may cause severe health problems especially for pregnant (congenital toxoplasmosis) and immunocompromised patients. This study aimed to verify the diagnostic significance of hematological parameters and C-reactive protein (CRP) for toxoplasmosis acute detection. A case-control study was carried out between December 2017 and May 2018, in samples of convenience independent of age and sex. The case group was formed by 25 patients with positive anti-Toxoplasma gondii IgG/IgM antibody and the control group was formed by 21 patients with non-positive anti-Toxoplasma gondii IgG/IgM antibody. The results of the hematological parameters and CRP were analyzed in these patients. The patients with Toxoplasma gondii IgM antibody reagent showed higher lymphocytes counting and lower neutrophils counting than the control group. C-reactive protein levels were not different between the groups case and control. ROC curve analysis highlighted that the cutoff value of [ 32.00% for lymphocytes and \ 57.50% for neutrophils were able to produce specificity higher than 90% for IgM antibody detection. The Naïve Bayes classifier was considered suitable (AUC & 0.700) to separate both groups according to their white cell counting. Changes in lymphocytes and neutrophils may be useful parameters for toxoplasmosis identification and may be used as a tool in the complementary diagnosis of toxoplasmosis.

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Serologic Tests of IgG and IgM Antibodies and IgG Avidity for Diagnosis of Ocular Toxoplasmosis

Cited by 26 publications

References 28 publications

Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran

Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis

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