Serological Activity of Lipids of a T strain of
            <i>Mycoplasma</i>

Romano, Nino; Scarlata, G

doi:10.1128/iai.9.6.1062-1065.1974

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…Our results demonstrate, too, that the lipids are able to adsorb and evoke CF antibodies. Such results are in agreement with our previous report (10), which showed the lipids' ability to react in the CF test with an antiserum against the whole organism.…”

Section: Resultssupporting

confidence: 94%

“…A gentle shaking was followed by sonic treatment for 4 to 5 min in a Biosonik II ultrasonic disintegrator. The immunization schedule was as previously described (10). Adsorption of antisera.…”

Section: Methodsmentioning

confidence: 99%

“…Serological tests. The CF test was carried out in a microtitration system, using the whole-cell suspension as antigen (10).…”

Section: Methodsmentioning

confidence: 99%

“…A previous report demonstrated that the CF activity of a T-strain of mycoplasma was associated with its own lipid component (10), and since virtually all the lipid content is located in the membrane (7), it is likely that this activity is membrane bound.…”

mentioning

confidence: 99%

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Immunological analysis of plasma membranes of a T-strain of mycoplasma (Ureaplasma urealyticum)

Romano¹,

Licata²,

Scarlata³

1977

Infect Immun

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The cell membranes of a T-strain of mycoplasma, obtained by ultrasonic disruption, were as effective as whole organisms in eliciting metabolism-inhibiting and complement-fixing antibodies. The soluble fraction separated from cell membranes by centrifugation at 35,000 X g showed a minor ability to elicit an antibody response as measured by metabolism inhibition and complement fixation tests. After a further centrifugation at 100,000 X g, the immunogenic activity of the soluble fraction was completely lost. Immunogenic determinants in mycoplasma membranes could also be demonstrated by adsorption tests; cell membranes were more effective than soluble fractions in adsorbing antibody capacity from the immune sera against whole cells. It has been shown by further experiments that cell membranes have at least two major antigenic determinants, which differ either in chemical nature or in capacity to adsorb and evoke antibodies, characterized by different serological behaviors.

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Section: Resultssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

“…Serological tests. The CF test was carried out in a microtitration system, using the whole-cell suspension as antigen (10).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Immunological analysis of plasma membranes of a T-strain of mycoplasma (Ureaplasma urealyticum)

Romano¹,

Licata²,

Scarlata³

1977

Infect Immun

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“…laidlawii was associated with the glycolipids monoglucosyl diglyceride, diglucosyl diglyceride, glycerylphosphoryl diglucosyl diglyceride, and an unknown lipid B, which did not react with ninhydrin but released glycerol, glucose, and traces of phosphorus upon hydrolysis. The glycolipids of M. pneumoniae that are di-and trihexosyldiglycerides containing galactose and/or glucose and the glycolipids of Tstrain mycoplasmas that have not been characterized have also been associated with the complement-fixing activity of their respective cells (21,30). Although glycolipids such as those from T-strain mycoplasmas are also immunologically active in passive hemagglutination tests, their serological activity is most reliably estimated by the complement fixation test because small amounts of insoluble antigens are reac- tive, isofixation curves can be prepared, and small quantities of antibodies are sensitively assayed.…”

Section: Resultsmentioning

confidence: 99%

Immunological properties of glycolipids from membranes of Acholeplasma laidlawii

Ryan¹,

Noker²,

Matz³

1975

Infect Immun

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Glycolipids, the predominant class of lipids in the membranes of Acholeplasma laidlawii, are the haptenic determinants that react with anti-A. Laidlawii serum to fix complement. The predominant complement-fixing activity of the membrane glycolipids was associated with the monoglucoysyl diglyceride, diglucosyl diglyceride, glycerlphosphoryl diglucosyl diglyceride (GPDD), and an unknown lipid B, which did not react with ninhydrin but release glucose and glycerol and traces of phosphorus upon hydrolysis. The glycolipids monoglucosyl diglyceride and diglucosyl diglyceride or GPDD and unknown lipid B were paired as a result of their cross-reactions with selective antisera prepared with the aid of reconstituted membrane complexes containing membrane lipids. Reconstituted membrane complexes assembled from [14C]monoglucosyl diglyceride and delipidated membrane proteins gave optimal complement fixation titers before saturation of the complexes with the ]14C]monoglucosyl diglyceride. The phosphoglycolipid of the membrane, GPDD, was anticomplementary as a pure lipid, a cholesterol liposome, and a reconstituted membrane complex. This anticomplementary activity, which was caused by 3 mug of pure GPDD, affected both human and guinea pig complement. Although human C1, C4, C3, and C5 were not inhibited by GPDD, C2 was inhibited 10-fold by reconstituted membrane complexes containing 150 mug of GPDD. A role for this phosphoglycolipid is discussed in the hypothetical mechanism of inhibition of C2 attachment to SAC1, 4 sites.

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